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2015-N iFAST

Plasmid

#209415

Purpose

iFAST expression plasmid using MET3p

Depositor

Patrick Van Dijck

Article

Devos et al Microbiology (Reading). 2024 Mar;170(3).

Insert

iFAST

Expression

Yeast

Promoter

MET3

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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NK804

Plasmid

#166058

Purpose

Puromycin resistance and MRLC-mTFP expression for Salpingoeca rosetta

Depositor

Nicole King

Article

Brunet et al Elife. 2021 Jan 15;10. pii: 61037. doi:

Insert

MRLC-mTFP

Use

S. rosetta expression

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pEft-3::Cerulean-Venus::Tomm-7

Plasmid

#68046

Purpose

Ubiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pEft-3::Cerulean-Venus::Tomm-7

Expression

Worm

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pRMH124-VP16-CIB1

Plasmid

#40615

Purpose

Expresses VP16-CIB1 for light-inducible protein expression when coupled with GalBD-CRY2PHR or GalBD-CRY2

Depositor

Chandra Tucker

Article

Hughes et al Methods. 2012 Aug 15.

Insert

VP16-CIB1

Expression

Yeast

Promoter

ADH1

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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NK802

Plasmid

#166056

Purpose

Puromycin resistance and mCherry expression for Salpingoeca rosetta

Depositor

Nicole King

Article

Brunet et al Elife. 2021 Jan 15;10. pii: 61037. doi:

Insert

Pac puromycin resistance gene

Use

S. rosetta expression

Tags

mCherry

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pEft-3::oxCerulean-oxVenus::Lgg-1

Plasmid

#68040

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed ubiquitously, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pEft-3::oxCerulean-oxVenus::Lgg-1

Expression

Worm

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pDpy-7::oxCerulean-oxVenus::Lgg-1

Plasmid

#68042

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in hypodermis, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pDpy-7::oxCerulean-oxVenus::Lgg-1

Expression

Worm

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2 xBtg-X

Plasmid

#24964

Depositor

Edward De Robertis

Article

Kuroda et al Genes Dev. 2005 May 1. 19(9):1022-7.

Insert

Btg-X (MGC76328.S Frog)

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pVha-6::oxCerulean-oxVenus::Lgg-1

Plasmid

#68041

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in intestines, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pVha-6::oxCerulean-oxVenus::Lgg-1

Expression

Worm

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pMyo-2::oxCerulean-oxVenus::Lgg-1

Plasmid

#68043

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in the pharynx, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pMyo-2::oxCerulean-oxVenus::Lgg-1

Expression

Worm

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6 - iFAST

Plasmid

#209414

Purpose

iFAST containing plasmid for endogenous fusion constructs

Depositor

Patrick Van Dijck

Article

Devos et al Microbiology (Reading). 2024 Mar;170(3).

Insert

iFAST

Expression

Yeast

Promoter

none

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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2016-N

Plasmid

#190044

Purpose

Backbone vector for generation of BiFC plasmids

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Type

Empty backbone

Expression

Yeast

Promoter

Met3

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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2016-C

Plasmid

#190045

Purpose

Backbone vector for generation of BiFC plasmids

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Type

Empty backbone

Expression

Yeast

Promoter

Met3

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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2015-C

Plasmid

#190043

Purpose

Backbone vector for generation of BiFC plasmids

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Type

Empty backbone

Expression

Yeast

Promoter

Met3

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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2015-N

Plasmid

#190042

Purpose

Backbone vector for generation of BiFC plasmids

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Type

Empty backbone

Expression

Yeast

Promoter

Met3

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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xyl3.1

Plasmid

#107209

Purpose

Xylanase design 3.1

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

xyl3.1

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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xyl3.2

Plasmid

#107210

Purpose

Xylanase design 3.2

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

xyl3.2

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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xyl3.3

Plasmid

#107211

Purpose

Xylanase design 3.3

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

xyl3.3

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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xyl4.1

Plasmid

#107212

Purpose

Xylanase design 4.1

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

xyl4.1

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
xyl4.2

Plasmid

#107213

Purpose

Xylanase design 4.2

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

xyl4.2

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
xyl8.1

Plasmid

#107214

Purpose

Xylanase design 8.1

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

xyl8.1

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
xyl8.2

Plasmid

#107216

Purpose

Xylanase design 8.2

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

xyl8.2

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
xyl8.3

Plasmid

#107217

Purpose

Xylanase design 8.3

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

xyl8.3

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMyo-3::oxCerulean-oxVenus::Lgg-1

Plasmid

#68044

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in body wall muscle, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pMyo-3::oxCerulean-oxVenus::Lgg-1

Expression

Worm

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-OVA

Plasmid

#64599

Purpose

Expression of chicken ovalbumin as model antigen for study of MHC class I and class II presentation

Depositor

Sandra Diebold, Martin Zenke

Article

Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

Insert

Ovalbumin

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ClopHensor

Plasmid

#25938

Depositor

Daniele Arosio

Article

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

ClopHensor

Tags

Strep-II

Expression

Mammalian

Mutation

E2GFP is EGFP-T203Y

Available Since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only

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pSUC01

Plasmid

#177204

Purpose

chromogenic reporter for gene knockout in Streptomyces

Depositor

Wenjun Guan

Article

Dong et al J Microbiol Biotechnol. 2021 Dec 28;31(1

Insert

SaindC

Use

Synthetic Biology

Tags

NONE

Expression

Bacterial

Mutation

NONE

Promoter

TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-Peredox-mCherry-NLS

Plasmid

#32385

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCherry-NLS

Use

Retroviral

Tags

Nuclear Localization Seq

Expression

Mammalian

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pOSV00170

Plasmid

#222934

Purpose

Constitutive GFP expression for Bacillus subtilis

Depositor

Ophelia Venturelli

Article

Cheng et al Nat Commun. 2023 Apr 10;14(1):2001. doi:

Insert

GFP(Sp)

Use

Synthetic Biology

Expression

Bacterial

Promoter

Phyperspank

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-HA-hTAF4b

Plasmid

#17998

Depositor

Robert Tjian

Article

Freiman et al Science. 2001 Sep 14. 293(5537):2084-7.

Insert

hTAF4b (TAF4B Human)

Tags

HA

Expression

Mammalian

Mutation

aa1-64 deleted

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
GW1-Peredox-mCherry-NLS

Plasmid

#32381

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCherry-NLS

Tags

Nuclear Localization Seq

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2 GSK3-GFP

Plasmid

#29680

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

glycogen synthase kinase 3

Tags

GFP

Expression

Mammalian

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pCS2 GSK3-RFP

Plasmid

#29679

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

glycogen synthase kinase 3

Tags

RFP

Expression

Mammalian

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
NK803

Plasmid

#166057

Purpose

Puromycin resistance and LifeAct-mCherry expression for Salpingoeca rosetta

Depositor

Nicole King

Article

Brunet et al Elife. 2021 Jan 15;10. pii: 61037. doi:

Inserts

Pac puromycin resistance gene
LifeAct-mCherry

Use

S. rosetta expression

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
DsRed-Rab5-QL

Plasmid

#29688

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

RAB5A

Tags

DSRed2

Expression

Mammalian

Mutation

Q79L

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
pCS2 DN-GSK3-GFP

Plasmid

#29681

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

glycogen synthase kinase 3

Tags

GFP

Expression

Mammalian

Mutation

K85R and K86R

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pAct1IHPT-4

Plasmid

#37844

Depositor

Peggy Lemaux

Article

Myeong-Je et al Volume 138, Issue 2, 23 November 1998, P

Insert

Actin promoter and intron

Use

Plant transformation

Tags

HPT (Hygromycin phosphotransferase) and nos 3…

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
JB97

Plasmid

#128926

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

RalBGV-BirA*-HA-MAO (RALB Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
JB98

Plasmid

#128927

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

RalBSA-BirA*-HA-MAO (RALB Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pll2.1

Plasmid

#107202

Purpose

PLL design 2.1

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

pll2.1 design

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pll2.2

Plasmid

#107203

Purpose

PLL design 2.2

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

PLL 2.2

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pll2.3

Plasmid

#107204

Purpose

PLL design 2.3

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

PLL 2.3

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pll2.4

Plasmid

#107205

Purpose

PLL design 2.4

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

PLL 2.4

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pll2.5

Plasmid

#107206

Purpose

PLL design 2.5

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

PLL 2.5

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pll3.1

Plasmid

#107207

Purpose

PLL design 3.1

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

PLL 3.1

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pll4.1

Plasmid

#107208

Purpose

PLL design 4.1

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

PLL 4.1

Expression

Bacterial

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJC53.2/Smed001246

Plasmid

#35693

Depositor

Phillip Newmark

Article

Rouhana et al Development. 2012 Mar;139(6):1083-94. Ep

Insert

Protein arginine methyltransferase 1 homolog

Promoter

Sp6 for sense, T7 for sense and antisense

Available Since

June 14, 2012

Availability

Academic Institutions and Nonprofits only
pJC53.2/Smed005105

Plasmid

#35694

Depositor

Phillip Newmark

Article

Rouhana et al Development. 2012 Mar;139(6):1083-94. Ep

Insert

Protein arginine methyltransferase 1 homolog

Promoter

Sp6 for sense, T7 for sense and antisense

Available Since

June 14, 2012

Availability

Academic Institutions and Nonprofits only
pCMV5-Smad7-HA

Plasmid

#11733

Depositor

Jeff Wrana

Article

Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

Insert

SMAD7 (SMAD7 Human)

Tags

HA

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDRF1 ymScarletI3_T2A_mTurquoise2

Plasmid

#219829

Purpose

equimolar expression of ymScarletI3 and mTurquoise2 in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ymScarletI3_T2A_mTurquoise2

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

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