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Plasmid#107383PurposeMammalian expression CRISPR/Cas9DepositorInsertsgRNA targeting CRBN (CRBN Human)
UseLentiviral; CrisprTagsExpressionMammalianMutationPromoterU6Available sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-RFP-IKZF3-sh3
Plasmid#69044Purpose3rd generation lentiviral vector expressing IKZF3 shRNADepositorInsertIKZF3 (IKZF3 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pROF141
Plasmid#155329PurposeBinary vector for expression of the PULSE system under the control of PAtUbi10 promoterDepositorInsertLB_PAtUbi10-SRDX-NLS-EL222-Tnos_SF_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF402
Plasmid#155334PurposeBinary vector for expression of FLuc controlled by the blue light-repressible systemDepositorInsertLB_P35Senhancer(-953 to -51)-(C120)5-PhCMVmin-FLuc-T35S_RB
UseLuciferase and Synthetic Biology; Eukaryotic expr…TagsExpressionMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF441
Plasmid#155336PurposeBinary vector for expression of a gRNA targeting AtAP1 promoterDepositorInsertLB_PAtU6-26-gRNA(PAtAP1)-sgRNA_RB
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO-RFP-IKZF3-sh2
Plasmid#69043Purpose3rd generation lentiviral vector expressing IKZF3 shRNADepositorInsertIKZF3 (IKZF3 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CohIII-CBM-HIS-ybbR
Plasmid#60866PurposeCharacteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tagDepositorInsertsCohIII
CBM
UseTags6xHIS, HRV 3C, and ybbRExpressionBacterialMutationCysteine 63 to Serine in CBM modulePromoterT7Available sinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-Myc-CRBN-pGK-HYG
Plasmid#107376PurposeLentiviral expression of myc-CRBNDepositorInsertcereblon (CRBN Human)
UseLentiviralTagsMycExpressionMammalianMutationPromoterUbcPAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-HA-YWAA-pGK-HYG
Plasmid#107379PurposeLentiviral expression of HA-YWAADepositorInsertcereblon (CRBN Human)
UseLentiviralTagsHAExpressionMammalianMutationCRBN-Y383A/W385APromoterUbcPAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-Myc-YWAA-pGK-HYG
Plasmid#107377PurposeLentiviral expression of myc-YWAADepositorInsertcereblon (CRBN Human)
UseLentiviralTagsMycExpressionMammalianMutationCRBN-Y383A/W385APromoterUbcPAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-FLAG-YWAA-pGK-HYG
Plasmid#107375PurposeLentiviral expression of FLAG-YWAADepositorInsertcereblon (CRBN Human)
UseLentiviralTagsFLAGExpressionMammalianMutationCRBN-Y383A/W385APromoterUbcPAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-UBC- IKZF5-3xHA-pGK-PUR
Plasmid#107385PurposeLentiviral expression of IKZF5-3xHADepositorInsertIKZF5 (IKZF5 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterUBCAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HIS-Xyl-Xmod-DocIII
Plasmid#60865PurposeCharacteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120CDepositorInsertsXyl
Xmod-DocIII
UseTags6xHISExpressionBacterialMutationThreonine 120 to Cysteine in Xylanase domainPromoterT7Available sinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
plenti-CAG-IKZF3-Q147H-FLAG-IRES-GFP
Plasmid#69050Purposelentiviral expression of IKZF3 with Q147H mutation and FLAG tagDepositorInsertIKZF3 (IKZF3 Human)
UseLentiviralTagsFLAG and IRES-GFPExpressionMammalianMutationQ147HPromoterCAGAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
PGK-LCB3_v1.2-NotchGal4VP64-T2A-mCherry (pZYW047)
Plasmid#194230PurposeLentiviral expression vector expressing LCB3 SynNotch and mCherry transduction markerDepositorInsertPGK-LCB3-SynNotch-T2A-mCherry
UseLentiviralTagsExpressionMutationE45D mutation in LCB3PromoterPGKAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-LCB1_v1.3-PDGFR-T2A-mCherry (pZYW048)
Plasmid#194231PurposeLentiviral expression vector expressing LCB1 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction markerDepositorInsertEF1a-LCB1-PDGFR TMD-T2A-mCherry
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-LCB3_v1.2-PDGFR-T2A-mCherry (pZYW049)
Plasmid#194232PurposeLentiviral expression vector expressing LCB3 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction markerDepositorInsertEF1a-LCB3-PDGFR TMD-T2A-mCherry
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
BM00654
Plasmid#155339PurposeBinary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette.DepositorInsertRB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-RLuc-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-FLuc-POpto_LB
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
plenti-CAG-IKZF1-V1-FLAG-IRES-GFP
Plasmid#107387PurposeLentiviral expression of IKZF1-V1-FLAGDepositorInsertIKZF1-V1 (IKZF1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCAGAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
eGFP-proα2(I)
Plasmid#119826PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with GFP between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagseGFPExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only