We narrowed to 2,924 results for: T2A

1. DHFR-PP7-VP64_T2A_GFP

   Plasmid

   #86167

   Purpose

   Expresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

   Insert

   DHFR-PP7-VP64_T2A_GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Destabilized domain (N-terminus version) of E.col…

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUG-T2A-Pten

   Plasmid

   #140738

   Purpose

   Lentiviral vector to overexpress human PTEN and GFP

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   GFP-PTA-PTEN (PTEN Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Promoter

   hUbiC

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. MCS:GFPnls-T2a-ER-Cre-ER

   Plasmid

   #200106

   Purpose

   Vector for LFNG

   Depositor

   Prayag Murawala

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #112922

   Purpose

   Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCas9-T2A-mApple

   Plasmid

   #193780

   Purpose

   Tricistronic expression of Cas9-mApple-BSD in mammalian cells

   Depositor

   James Eshleman

   Article

   Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

   Insert

   mApple
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   In frame with Cas9

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2

   Plasmid

   #199486

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   Gal4vp16/4xnrUAS-mTagBFP2
   

   Use

   CRISPR

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2Aneo-tdTomato-CAAX

   Plasmid

   #170284

   Purpose

   Encoding membrane-anchored tdTomato.

   Depositor

   Michiyuki Matsuda

   Article

   Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

   Insert

   tdTomato-CAAX
   

   Expression

   Mammalian

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_tKiMBI-T2A-caMEK

   Plasmid

   #199578

   Purpose

   Expresses tKiMBI and caMEK in an AAV vector

   Depositor

   Michael Lin

   Article

   Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

   Inserts

   ERK tdTomato-Kinase-Modulated Bioluminescent Indicator
   constitutively active MEK
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. mT2A-tdTomato-FNF

   Plasmid

   #182340

   Purpose

   Recombineering cassette plasmid for T2A-tdTomato-FNF

   Depositor

   Mario Capecchi

   Insert

   T2A-tdTomato-FNF
   

   Use

   Mouse Targeting; Flp / frt

   Expression

   Mammalian

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Fuc-T2A-cre

    Plasmid

    #66690

    Purpose

    Recombine floxed alleles in any cell

    Depositor

    Bryan Luikart

    Article

    Williams et al J Neurosci. 2015 Jan 21;35(3):943-59. do

    Insert

    mCherry-T2A-Cre
    

    Use

    Lentiviral

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2

    Plasmid

    #199485

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    Gal4vp16/4xnrUAS-mTagBFP2
    

    Use

    CRISPR

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113344

    Purpose

    Expresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    VP64-dCas9-p300core-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-R1R2_EF1adCas9p300_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113342

    Purpose

    Expresses dCas9-p300 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    dCas9-p300core-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA5/FRT/TO_HaloTag9_T2A_EGFP

    Plasmid

    #169326

    Purpose

    Expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag9
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA

    Plasmid

    #112921

    Purpose

    Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    EF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
    

    Use

    Gateway entry vector

    Expression

    Mammalian

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. FUG-T2A-Cas9

    Plasmid

    #75346

    Purpose

    Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP (via t2a)

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT2A-EKAREV-NLS

    Plasmid

    #173856

    Purpose

    Encoding EKAREV-NLS.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    EKAREV-NLS
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTAPmyc-T2A-tagRFP

    Plasmid

    #140278

    Purpose

    Backbone for making a mammalian expression vector of the protein of interest

    Depositor

    Hirohide Saito

    Article

    Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

    Insert

    Empty backbone
    

    Use

    Synthetic Biology

    Tags

    myc-T2A-tagRFP

    Expression

    Mammalian

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pF3BGX-T2A-GAL4

    Plasmid

    #138392

    Purpose

    RMCE vector for generating C-terminal T2A-GAL4 fusion

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-GAL4
    

    Expression

    Insect

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. iBlueberry T2A mCherry

    Plasmid

    #72434

    Purpose

    Expressing of iBlueberry and mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Yu et al Protein Sci. 2016 Feb;25(2):308-15. doi:

    Insert

    iBlueberry T2A mCherry
    

    Expression

    Mammalian

    Mutation

    mutation E177Q in iBlueberry

    Available Since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only