-
Plasmid#155241Purposefor mammalian overexpression of truncated (delta1-536) ER isoform of rat USP19DepositorInsertUSP19 (delta1-536) (ER isoform) (Usp19 Rat)
UseTags6XHis-3XFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRARB.1.0-gDNA
Plasmid#132471PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertRARB (RARB Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK275
Plasmid#73363PurposeEncodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)DepositorInsertdelta-8 sphingolipid desaturase
UseTagsExpressionYeastMutationmissing residues 1-6PromoterGAL1Available sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK521
Plasmid#72440PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)DepositorInserthypothetical protein
UseUnspecifiedTagsExpressionMutationsynthetic gene with ~40 silent substitutionsPromotern/aAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK323
Plasmid#73495PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide synthetaseDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK536
Plasmid#72444PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)DepositorInserthypothetical protein
UseTagsExpressionBacterialMutationsynthetic gene with ~40 silent substitutionsPromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK547
Plasmid#72449PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F)DepositorInsertN5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationVal319 to PhePromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK550
Plasmid#72450PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationinserted Val2PromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
LHP809 - Ub-K63A-CYC1
Plasmid#32166DepositorUseTagsExpressionYeastMutationK63APromoterCUP1Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
LHP327 - Ub-F45A/G76A
Plasmid#32165DepositorUseTagsExpressionYeastMutationF45A and G76APromoterCUP1Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-NT*-HRV3CP
Plasmid#162795PurposeExpressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)DepositorInsertHuman rhinovirus 14 3C protease
UseTagsNT* solubility tagExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGilesSHdI_redgreen_2395A_His6_triple red_pLeft1806_His6_double green
Plasmid#170048PurposeTri-Responsive Reporter Gene Construct for redgreen, triple red, and double greenDepositorInsertsmScarlet
COmScarlet
mRuby3
mWasabi dN
mWasabi CO
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSGV Mouse Acceptor
Plasmid#128176PurposeA TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR ref Biotechniques. 2015 Mar 1;58(3):135-9DepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterMSCVAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Plasmid L5 attB::Pleft* mScarlet
Plasmid#169410PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mScarletDepositorInsertspLeft
mScarlet proper
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plasmid L5 attB::Pleft* mWasabi
Plasmid#169409PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mWasabiDepositorInsertspLeft
mWasabi
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plasmid L5 attB::Pleft* mCherry
Plasmid#169411PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mCherryDepositorInsertspLeft
mCherry
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plasmid L5 attB:Psmyc double blue
Plasmid#170050PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double blueDepositorInsertsmTagBFP2
CO BFP
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plasmid L5 attB:Psmyc double green
Plasmid#169412PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double greenDepositorInsertsmWasabi
mWasabi CO
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plasmid L5 attB:Psmyc triple red
Plasmid#169413PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for triple redDepositorInsertsmScarlet proper
COmScarlet
mRuby3
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET151-D-TOPO-PARP14-SΔK
Plasmid#216658PurposeExpresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.DepositorInsertPARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Synthetic, Human)
UseTags6xHis-V5-TEVExpressionBacterialMutationDeletion starting from Q1390 to D1448 and substit…PromoterT7Available sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only