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Showing: 401 - 420 of 16731 results

  1. pLenti GW V5 Eco/Dam mse EMD

    Plasmid
    #182675
    Purpose
    Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Emerin. Used in DamID experiments to methylate DNA that interacts in the proximity of Emerin.
    Depositor
    Bas van Steensel
    Article
    Amendola et al EMBO Rep. 2015 May;16(5):610-7. doi: 10.
    Insert
    Emerin (Emd Mouse)
    Use
    Lentiviral
    Tags
    E. Coli Dam and V5
    Expression
    Mammalian
    Mutation
    Promoter
    HSP
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182675-plasmid-map-sequence-id-358621

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  6. pRS405 SEC66-VAC8-RFP

    Plasmid
    #166843
    Purpose
    Expresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.
    Depositor
    Daniel Klionsky
    Article
    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554
    Insert
    VAC8 (VAC8, SEC66 Budding Yeast)
    Use
    Tags
    RFP and SEC66 transmembrane domain
    Expression
    Yeast
    Mutation
    SEC66 transmembrane domain
    Promoter
    VAC8 promoter
    Available since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166843-plasmid-map-sequence-id-329331

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  7. pCAGGS_m-AKAR5’

    Plasmid
    #114140
    Purpose
    Mammalian expression of the AKAR5’ sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    AKAR5' C-terminally tagged by KRas C tail
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    114140-plasmid-map-sequence-id-215469

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  16. pRS405 SEC66-VAC8-GFP

    Plasmid
    #166840
    Purpose
    Expresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.
    Depositor
    Daniel Klionsky
    Article
    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554
    Insert
    VAC8 (VAC8, SEC66 Budding Yeast)
    Use
    Tags
    GFP and SEC66 transmembrane domain
    Expression
    Yeast
    Mutation
    SEC66 transmembrane domain
    Promoter
    VAC8 promoter
    Available since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166840-plasmid-map-sequence-id-329328

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  19. pCAGGS_m-AKAR5

    Plasmid
    #114139
    Purpose
    Mammalian expression of the AKAR5 sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    AKAR5 C-terminally tagged by KRas C tail
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    114139-plasmid-map-sequence-id-215468

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Showing: 401 - 420 of 16731 results