We narrowed to 1,029 results for: gibson assembly

1. FFiBlast p5

   Plasmid

   #68470

   Purpose

   Inverted and floxed blasticidin resistance

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Blasticidin Resistance
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Inverted

   Promoter

   LTR

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

   Plasmid

   #185687

   Purpose

   Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERmembrane-GST-2xHaloTag-KKMP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFP.R400

   Plasmid

   #138175

   Purpose

   Expresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(GGK).NpuSsp*.(CWN)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   P(araBAD) and iPC

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE80L TriCatcher-RGDSP-MMP

   Plasmid

   #112633

   Purpose

   Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcher

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   TriCatcher-RGDSP-MMP
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   central matrix metalloproteinase-cleavable sequen…

   Promoter

   T5

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. TOPO iRFP670

   Plasmid

   #68443

   Purpose

   TOPO construct expressing iRFP670 for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   iRFP670
   

   Use

   Gmap

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFP.R375

   Plasmid

   #138245

   Purpose

   Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   P(araBAD) and iPC

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TOPO mKO2

   Plasmid

   #68442

   Purpose

   TOPO construct expressing mKusabira-Orange2 for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   mKusabira-Orange2
   

   Use

   Gmap

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP

   Plasmid

   #185686

   Purpose

   Low-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERmembrane-GST-HaloTag-KKMP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EZ-L767

   Plasmid

   #131773

   Purpose

   PADH1_sFUS_Fusion Red_PixD_TACT1

   Depositor

   Jared Toettcher

   Article

   Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

   Insert

   PADH1_sFUS_Fusion Red_PixD_TACT1
   

   Use

   Integration into δ-sites

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PADH1

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFP.R271

    Plasmid

    #138220

    Purpose

    Expresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(IDE).SspDnaX.(CHT)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TOPO Venus

    Plasmid

    #68493

    Purpose

    TOPO construct expressing Venus for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    split Venus
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EZ-L498

    Plasmid

    #131771

    Purpose

    PPGK1_FUSN_Citrine _PixE_TCYC1

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    PPGK1_FUSN_Citrine _PixE_TCYC1
    

    Use

    Integration into his3 locus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQE80L SpyTag-ELP-SpyTag DK

    Plasmid

    #112635

    Purpose

    SpyTag linked by an elastin-like polypeptide to non-reactive SpyTag DK

    Depositor

    Mark Howarth

    Article

    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

    Insert

    SpyTag-ELP-SpyTag DK
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Contains central cysteine. Reactive aspartic acid…

    Promoter

    T5

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFP.R365

    Plasmid

    #138235

    Purpose

    Expresses the split mCherry reporter interrupted by the EP-Pri artificially mini-intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(HGK).EP-Pri*.(TEV)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TOPO CloverCP

    Plasmid

    #68444

    Purpose

    TOPO construct expressing CloverCP for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    CloverCP
    

    Use

    Gmap

    Tags

    "CP" degradation domain

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TOPO mT2

    Plasmid

    #68491

    Purpose

    TOPO construct expressing mT2 for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    mTurquoise2
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSuper TR G414C

    Plasmid

    #207612

    Purpose

    U3 promoter driven expression of the telomerase RNA with a G414C CAB box mutation

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    Telomerase RNA G414C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U1

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET26b(modified)-T-Lg

    Plasmid

    #216977

    Purpose

    Expression of GvpT-Linker-Large BiT

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    GvpT-Linker-Large BiT
    

    Use

    Tags

    Split Nanoluciferase LargeBiT-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pST39-Mega'-deltaRLS

    Plasmid

    #216955

    Purpose

    Mega' with knockout of GvpR, GvpL and GvpS

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Mega' with knockout of GvpR, GvpL and GvpS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pST39-Mega'-deltaBSU

    Plasmid

    #216953

    Purpose

    Mega' with knockout of GvpB, GvpS and GvpU

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Mega' with knockout of GvpB, GvpS and GvpU
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge