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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,124 results for: gibson assembly

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Showing: 401 - 420 of 1124 results

  1. P34m

    Plasmid
    #120945
    Purpose
    MoClo golden gate assembly AB part (low-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    P13 from Mutalik library (weak)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120945-plasmid-map-sequence-id-238039

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  2. pICH47732:FCP:ShBle

    Plasmid
    #85985
    Purpose
    Expresses ShBle with FCP promoter/terminator for F. cylindrus transformation.
    Depositor
    Thomas Mock
    Insert
    ShBle
    Use
    Golden gate assembly
    Promoter
    FCP
    Available Since
    Jan. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    85985-plasmid-map-sequence-id-169710

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  5. pFP.R270

    Plasmid
    #138219
    Purpose
    Expresses the split mCherry reporter interrupted by the IMPDH-1 intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(GGG).IMPDH-1.(SIC)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138219-plasmid-map-sequence-id-273501

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  7. pFP.R269

    Plasmid
    #138181
    Purpose
    Expresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(NPC).NrdJ-1.(SEI)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    138181-plasmid-map-sequence-id-296586

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  8. pFP.R268

    Plasmid
    #138180
    Purpose
    Expresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(LNR).gp41-8.(SAV)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138180-plasmid-map-sequence-id-273503

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  10. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

    Plasmid
    #216283
    Purpose
    Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    SpyCatcher003-TEVs-SpyTag003DA
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216283-plasmid-map-sequence-id-433206

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  12. pFP.R267

    Plasmid
    #138179
    Purpose
    Expresses the split mCherry reporter interrupted by the gp41-1 intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(SGY).gp41-1.(SSS)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138179-plasmid-map-sequence-id-273462

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  13. pET21b-MBPx-MBPx-SpyTag003

    Plasmid
    #216291
    Purpose
    Expresses tandem MBPx linked to-SpyTag003 in bacterial cells. MBPx is a variant of MBP (A312V I317V Δ172-173 Δ175-176) with enhanced affinity for maltose.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    MBPx-MBPx-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 and SpyTag003
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    216291-plasmid-map-sequence-id-443903

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  15. pFP.R377

    Plasmid
    #138247
    Purpose
    Expresses the split mCherry reporter interrupted by the Mja-KlbA intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(HDG).Mja-KlbA*.(CSG)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138247-plasmid-map-sequence-id-273495

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  16. p006-GFP-Logic-AND

    Plasmid
    #112237
    Purpose
    GFP from jellyfish Aquorea sp. under D61 AND gate-like ara+lac promoter with AraC repressor; lacI repressor; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Inserts
    GFP under control of doubly-repressible promoter
    GFP
    araC
    Expression
    Bacterial
    Promoter
    AND-gate and pLacI
    Available Since
    Aug. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    112237-plasmid-map-sequence-id-218819

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  18. pFP.R265

    Plasmid
    #138167
    Purpose
    Expresses the split mCherry reporter interrupted by the SspDnaB M86 artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(VDA).M86.(SDL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138167-plasmid-map-sequence-id-273521

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Showing: 401 - 420 of 1124 results