We narrowed to 1,086 results for: gibson assembly
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Plasmid#185678PurposeLow-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.DepositorInsertERmembrane-HaloTag-KKMP
ExpressionMammalianAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFP.R265
Plasmid#138167PurposeExpresses the split mCherry reporter interrupted by the SspDnaB M86 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOPO mTagBFP2
Plasmid#68413PurposeTOPO construct expressing mTagBFP2 for generation of GMAP-compatible geneDepositorInsertmTagBFP2
UseGmapTags6xHisExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFP.R272
Plasmid#138221PurposeExpresses the split mCherry reporter interrupted by the SspGyrB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R364
Plasmid#138234PurposeExpresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOPO CFP
Plasmid#68489PurposeTOPO construct expressing CFP for generation of GMAP-compatible geneDepositorInsertCyan Fluorescent Protein
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO PuroR
Plasmid#68447PurposeTOPO construct expressing PuroR for generation of GMAP-compatible geneDepositorInsertPuromycin Resistance
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFP.R401
Plasmid#138222PurposeExpresses the split mCherry reporter interrupted by the TerThyX artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R360
Plasmid#138230PurposeExpresses the split mCherry reporter interrupted by the Ama-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOPO ZeoR
Plasmid#68446PurposeTOPO construct expressing ZeoR for generation of GMAP-compatible geneDepositorInsertZeocin Resistance
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO HygroR
Plasmid#68445PurposeTOPO construct expressing HygroR for generation of GMAP-compatible geneDepositorInsertHygromycin Resistance
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFP.R371
Plasmid#138241PurposeExpresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
FFiBlast p5
Plasmid#68470PurposeInverted and floxed blasticidin resistanceDepositorInsertBlasticidin Resistance
UseCre/LoxExpressionMammalianMutationInvertedPromoterLTRAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO iRFP670
Plasmid#68443PurposeTOPO construct expressing iRFP670 for generation of GMAP-compatible geneDepositorInsertiRFP670
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP-MMP
Plasmid#112633PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP-MMP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP
Plasmid#185687PurposeLow-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).DepositorInsertERmembrane-GST-2xHaloTag-KKMP
ExpressionMammalianAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFP.R400
Plasmid#138175PurposeExpresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R375
Plasmid#138245PurposeExpresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOPO mKO2
Plasmid#68442PurposeTOPO construct expressing mKusabira-Orange2 for generation of GMAP-compatible geneDepositorInsertmKusabira-Orange2
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
EZ-L767
Plasmid#131773PurposePADH1_sFUS_Fusion Red_PixD_TACT1DepositorInsertPADH1_sFUS_Fusion Red_PixD_TACT1
UseIntegration into δ-sitesExpressionYeastPromoterPADH1Available SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only