We narrowed to 24,746 results for: CHI

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pGBW-m4046826

Plasmid

#145687

Purpose

Bacterial Expression plasmid for SARS-CoV-2 nsp8

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

April 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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PX330_ZBTB7B_2

Plasmid

#135747

Purpose

Encodes gRNA for 3' target of human ZBTB7B

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB7B gRNA (ZBTB7B Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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PX330_ZBTB7B_1

Plasmid

#135746

Purpose

Encodes gRNA for 3' target of human ZBTB7B

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB7B gRNA (ZBTB7B Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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INPP5A / type I IP3 5-phosphatase from dog thyroid

Plasmid

#131767

Purpose

Expression in bacteria or COS-7 cells

Depositor

Christophe Erneux

Article

Verjans et al Biochem J. 1994 May 15;300 ( Pt 1):85-90

Insert

INPP5A

Expression

Bacterial

Available Since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.178

Plasmid

#119645

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7942 NS3 Down Flank

Use

Synthetic Biology

Mutation

4 nucleotide insertion between nt 65 and 66 in NS…

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.165

Plasmid

#119634

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

6803 NS1 Up Flank

Use

Synthetic Biology

Mutation

1 nucleotide insertion between nt 21 and 22 in NS…

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_X_EcSSPB-micro

Plasmid

#131218

Purpose

Expresses EcSSPB-nano (Escherichia coli) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

EcSSPB-micro

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_X_EcSSPB-nano

Plasmid

#131219

Purpose

Expresses EcSSPB-micro (Escherichia coli) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

EcSSPB-nano

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_EcSSPB-micro_X

Plasmid

#131213

Purpose

Expresses EcSSPB-micro (Escherichia coli) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

EcSSPB-micro

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.176

Plasmid

#119644

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7942 NS2 Down Flank

Use

Synthetic Biology

Mutation

bp 302 T to C

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.175

Plasmid

#119643

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7942 NS2 Up Flank

Use

Synthetic Biology

Mutation

bp 422 A to T, bp 423 A to T, bp 663 inserted G. …

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.174

Plasmid

#119641

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7942 NS1 Down Flank

Use

Synthetic Biology

Mutation

bp 658 G to A

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.171

Plasmid

#119639

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

6803 NS4 Up Flank

Use

Synthetic Biology

Mutation

A691T in NS4 Up Flank

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.169

Plasmid

#119637

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

6803 NS3 Up Flank

Use

Synthetic Biology

Mutation

bp 551 T to C, bp 554 T to C

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.018

Plasmid

#119622

Purpose

Level 0 Part. CDS

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

dCas9+deg-tag

Use

Synthetic Biology

Mutation

bp 331 C to A , 1237 A to C, 1284 C to T, 2121 C …

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.054

Plasmid

#119572

Purpose

Level 0 part. Promoter

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

PpetE

Use

Synthetic Biology

Mutation

RBS* added (22 bp) upstream of ATG. Deletion of n…

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.055

Plasmid

#119566

Purpose

Level 0 part. Promoter

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

PisiAB

Use

Synthetic Biology

Mutation

bp 391 G to A, RBS* added (22 bp) upstream of ATG…

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.057

Plasmid

#119565

Purpose

Level 0 part. Promoter

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ParsB

Use

Synthetic Biology

Mutation

bp 117 G to A, RBS* added (22 bp) upstream of ATG.

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCAT.334

Plasmid

#119556

Purpose

Level T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.079

Plasmid

#119667

Purpose

Level 0 part. Terminator for the psbA2 gene Synechocystis sp. PCC 6803.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

TpsbA2

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.066

Plasmid

#119666

Purpose

Level 0 part. Terminator from pheA gene from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

TpheA-1

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.080

Plasmid

#119665

Purpose

Level 0 part. Terminator for the cpcG1 gene Synechocystis sp. PCC 6803.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

TcpcG1

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.063

Plasmid

#119664

Purpose

Level 0 part. Terminator derived from Biobricks plasmid pSB1AK3.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

pSB1AK3 terminator

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.074

Plasmid

#119663

Purpose

Level 0 part. Terminator from Cysteine synthase A gene (cysK) from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ECK120048902

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.071

Plasmid

#119662

Purpose

Level 0 part. Terminator from tatABCD operon from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ECK120030798

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.069

Plasmid

#119661

Purpose

Level 0 part. Terminator from arcA gene from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ECK120026481

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.073

Plasmid

#119660

Purpose

Level 0 part. Terminator from rplJL-rpoBC,rplKAJL-rpoBC,rpoBC gene cluster from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ECK120010869

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.072

Plasmid

#119657

Purpose

Level 0 part. Terminator from hyaABCDEF operon from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ECK120010842

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pC0.077

Plasmid

#119656

Purpose

Level 0 part. Terminator from rpoH gene from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ECK120010841-R

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.064

Plasmid

#119655

Purpose

Level 0 part. Terminator from hupB gene from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

ECK120010820

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.070

Plasmid

#119652

Purpose

Level 0 part. Terminator, Bi-directional terminator for yciA/tonA genes from E. coli.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

BBa_B0061

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_M42FDHFR_WTTS

Plasmid

#91237

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

M42F

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_M42FG121VDHFR_R166QTS

Plasmid

#91238

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

M42FG121 and R166Q

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_M42FG121VDHFR_WTTS

Plasmid

#91239

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

M42FG121V

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_W22HDHFR_R166QTS

Plasmid

#91240

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

R166Q and W22H

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_W22HDHFR_WTTS

Plasmid

#91241

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

W22H

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_WTEcoliDHFR_R166QTS

Plasmid

#91242

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

R166QTS

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_D27NDHFR_R166QTS

Plasmid

#91243

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

D27N and R166Q

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_F31VDHFR_R166QTS

Plasmid

#91245

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

F31V and R166Q

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_F31VDHFR_WTTS

Plasmid

#91227

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

F31V

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_F31YG121VDHFR_R166QTS

Plasmid

#91228

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

F31YG121 and R166Q

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_F31YL54IDHFR_R166QTS

Plasmid

#91230

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

F31YL54I and R166Q

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_F31YL54IDHFR_WTTS

Plasmid

#91231

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

F31YL54I

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_G121VDHFR_R166QTS

Plasmid

#91232

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

G121V and R166Q

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_L54IDHFR_R166QTS

Plasmid

#91234

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

L54I and R166Q

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_L54IDHFR_WTTS

Plasmid

#91235

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

L54I

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_M42FDHFR_R166QTS

Plasmid

#91236

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

M42F and R166Q

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBac(3xP3-gTc’v; Valcyrie.LR)

Plasmid

#119066

Purpose

Valcyrie construct for CRISPR-mediated lox excision in Tribolium castaneum (high)

Depositor

Michalis Averof

Article

Gilles et al Development. 2019 Jan 4;146(1). pii: dev

Inserts

pBacL
Tc-U6a-Lox.L
Tc-hsp68 Promoter
NLS-Cas9
Tc-hsp68 3'UTR
Tc-U6B-Lox.R
SV40polyA
Tc-vermilion
3xP3 TATA
pBacR

Expression

Insect

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PCDHGC5 sh1 shRNA

Plasmid

#122227

Purpose

Knock Down Protocadherin gamma C5. It targets Protocadherin gamma C5 mRNA (nucleotides 851–871 in the protein coding region).

Depositor

Angel de Blas

Article

Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

Insert

PCDHGC5 shRNA (Pcdhgc5 Rat)

Use

RNAi

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PCDHGC5 sh1 3m shRNA

Plasmid

#122228

Purpose

Negative control shRNA (with 3-point mutations), for plasmid 122227: targeting Protocadherin gamma C5 mRNA.

Depositor

Angel de Blas

Article

Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

Insert

PCDHGC5 shRNA (Pcdhgc5 Rat)

Use

RNAi

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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