Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 11,232 results for: ENA

Showing: 10451 - 10500 of 11232 results

  2. pENTR-R4R3-2xmEGFP-N

    Plasmid
    #113747
    Purpose
    Gateway multi-site entry clone for second position 2x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.
    Depositor
    Magdalena Bezanilla
    Article
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    mEGFP-mEGFP
    Use
    CRISPR; Gateway entry vector
    Tags
    2xmEGFP
    Expression
    Mutation
    Promoter
    Available since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113747-plasmid-map-sequence-id-220024

    Enlarge

  3. pENTR-R4R3-3xmEGFP-N

    Plasmid
    #113748
    Purpose
    Gateway multi-site entry clone for second position 3x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.
    Depositor
    Magdalena Bezanilla
    Article
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    mEGFP-mEGFP-mEGFP
    Use
    CRISPR; Gateway entry vector
    Tags
    3xmEGFP
    Expression
    Mutation
    Promoter
    Available since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113748-plasmid-map-sequence-id-238958

    Enlarge

  4. pENTR-R4R3-2xmEGFP-C

    Plasmid
    #113750
    Purpose
    Gateway multi-site entry clone for second position 2x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.
    Depositor
    Magdalena Bezanilla
    Article
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    mEGFP-mEGFP
    Use
    CRISPR; Gateway entry vector
    Tags
    2xmEGFP
    Expression
    Mutation
    Promoter
    Available since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113750-plasmid-map-sequence-id-238960

    Enlarge

  5. pENTR-R4R3-2xmRuby-N

    Plasmid
    #113753
    Purpose
    Gateway multi-site entry clone for second position 2x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.
    Depositor
    Magdalena Bezanilla
    Article
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    mRuby2-mRuby2
    Use
    CRISPR; Gateway entry vector
    Tags
    2xmRuby2
    Expression
    Mutation
    Promoter
    Available since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113753-plasmid-map-sequence-id-238965

    Enlarge

  6. pENTR-R4R3-2xmRuby-C

    Plasmid
    #113756
    Purpose
    Gateway multi-site entry clone for second position 2x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.
    Depositor
    Magdalena Bezanilla
    Article
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    mRuby2-mRuby2
    Use
    CRISPR; Gateway entry vector
    Tags
    2xmRuby2
    Expression
    Mutation
    Promoter
    Available since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113756-plasmid-map-sequence-id-238973

    Enlarge

  9. YORWΔ22α2

    Plasmid
    #101707
    Purpose
    GoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci
    Depositor
    Elena Caro Bernat
    Article
    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do
    Type
    Empty backbone
    Use
    Yeast expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only

  12. YPRCΔ15α2

    Plasmid
    #101711
    Purpose
    GoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci
    Depositor
    Elena Caro Bernat
    Article
    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do
    Type
    Empty backbone
    Use
    Yeast expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only

  15. BDH2

    Plasmid
    #39151
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BDH2 (BDH2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. pIRE4-Azi

    Plasmid
    #105829
    Purpose
    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells
    Depositor
    Irene Coin
    Article
    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.
    Inserts
    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y37L, D182S, F183M, L186A, D265R)TyrRS
    Promoter
    PGK and U6
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105829-plasmid-map-sequence-id-203267

    Enlarge

  21. pET28-AviTag-DogCatcher-MBP

    Plasmid
    #171928
    Purpose
    Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    AviTag-DogCatcher-MBP
    Use
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    DogCatcher added to N-terminus of MBP via a GSGES…
    Promoter
    T7
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171928-plasmid-map-sequence-id-338570

    Enlarge

  22. pAC-CANTHipi

    Plasmid
    #53301
    Purpose
    Contains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    crtO
    Use
    Low copy number bacterial cloning vector
    Tags
    6His
    Expression
    Mutation
    Promoter
    Trc
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53301-plasmid-map-sequence-id-99820

    Enlarge

  23. L1-neo-TET

    Plasmid
    #51284
    Purpose
    expresses codon optimized human L1 retrotransposon driven by CMV promoter and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Article
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    L1RE1 (L1RE1 Human)
    Use
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    51284-plasmid-map-sequence-id-78366

    Enlarge

  25. pRDA_526

    Plasmid
    #184859
    Purpose
    Lentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.
    Insert
    sgRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    184859-plasmid-map-sequence-id-362663

    Enlarge

  30. SZ_EC_02

    Plasmid
    #155341
    Purpose
    Overexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XR
    Depositor
    Jens Nielsen
    Article
    Konzock et al BMC Microbiol. 2021 Mar 8;21(1):77. doi:
    Inserts
    tef1 promoter - xylulose kinase - pex20 terminator
    pyk1 promoter - xylose reductase - pex16 terminator
    gapdh promoter - xylitol dehydrogenase - lip2 terminator
    Use
    Easycloneyali insert vector
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    gapdh, pyk1, and tef1
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    155341-plasmid-map-sequence-id-316921

    Enlarge

  35. Opto-CRAC version 1

    Plasmid
    #101245
    Purpose
    Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling
    Depositor
    Yubin Zhou
    Article
    He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1
    Insert
    LOV2 and STIM1 cytosolic fragment
    Use
    Tags
    mCherry
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    101245-plasmid-map-sequence-id-209272

    Enlarge

  36. YIP sHRPa-Aga1P

    Plasmid
    #73151
    Purpose
    Large fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Insert
    sHRPa-Aga1P
    Use
    Tags
    V5 tag
    Expression
    Yeast
    Mutation
    Promoter
    glyceraldehyde-3-phosphate dehydrogenase GPD prom…
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73151-plasmid-map-sequence-id-134538

    Enlarge

  43. pMH-Cas9-gate

    Plasmid
    #113742
    Purpose
    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.
    Depositor
    Magdalena Bezanilla
    Article
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    Cas9
    Use
    CRISPR; Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Z. Mays ubiquitin promoter, 35S promoter
    Available since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113742-plasmid-map-sequence-id-238953

    Enlarge

  44. mCherry-OptoPB

    Plasmid
    #113935
    Purpose
    Express a LOV2 fusion protein to enable light-dependent cytosol-to-PM translocation and dissect protein-phosphoinositide interactions
    Depositor
    Yubin Zhou
    Article
    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi
    Insert
    OptoPB
    Use
    Tags
    mCherry
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    113935-plasmid-map-sequence-id-367689

    Enlarge

  46. pDN80

    Plasmid
    #61349
    Purpose
    LINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.
    Depositor
    Roland Eils, Barbara Di Ventura
    Article
    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    AsLOV2 wt and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    61349-plasmid-map-sequence-id-99714

    Enlarge

  47. pMK-Cas9-gate

    Plasmid
    #113743
    Purpose
    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.
    Depositor
    Magdalena Bezanilla
    Article
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    Cas9
    Use
    CRISPR; Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Z. Mays ubiquitin promoter, 35S promoter
    Available since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113743-plasmid-map-sequence-id-220965

    Enlarge

Showing: 10451 - 10500 of 11232 results