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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,094 results for: pcdna3.1

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Showing: 4181 - 4200 of 7094 results

  2. FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

    Plasmid
    #137830
    Purpose
    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factor
    Depositor
    Joshua Leonard
    Publication
    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Mutated Leucine 71 to Lysine in CTEVp fragment
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    137830-plasmid-map-sequence-id-311956

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  3. SpyTag-RBD

    Plasmid
    #159999
    Purpose
    Expresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells
    Depositor
    Mark Howarth
    Publication
    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    159999-plasmid-map-sequence-id-313235

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  4. MmXYRS-4xM15

    Plasmid
    #155343
    Purpose
    Plasmid encoding the translational pair that enables efficient incorporation of multiple phenylalanine-based amino acids in response to the amber codon into proteins in mammmalian cells.
    Depositor
    Irene Coin
    Publication
    Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15
    Insert
    Humanized MmXYRS/tRNAM15
    Expression
    Mammalian
    Promoter
    CMV/U6
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155343-plasmid-map-sequence-id-310521

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  5. pcDNA3-sACE2v2.4(732)-8h

    Plasmid
    #154103
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (8his tag)
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Short GSG linker and 8his tag
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-G…
    Promoter
    CMV
    Available since
    June 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    154103-plasmid-map-sequence-id-301646

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  6. pcDNA3-sACE2v1-Fc(IgG1)

    Plasmid
    #145164
    Purpose
    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity variant 1
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Fc region of human IgG1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145164-plasmid-map-sequence-id-292577

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  7. Calreticulin-SuperNova-KDEL

    Plasmid
    #131857
    Purpose
    Expresses SuperNova fused to ER protein calreticulin and KDEL ER-retention signal
    Depositor
    Geert van den Bogaart
    Publication
    Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SuperNova
    Tags
    KDEL ER-retention signal and Signal sequence of h…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131857-plasmid-map-sequence-id-261768

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  9. H2B-mGarnet2

    Plasmid
    #104312
    Purpose
    codes for a fusion of fluorescent protein mGarnet2 and human histon 2B (H2B), makes the H2B visible in live cells
    Depositor
    Ulrich Nienhaus
    Publication
    Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2B-mGArnet2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    104312-plasmid-map-sequence-id-225638

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  11. Rlucrarr3-Flash2

    Plasmid
    #74130
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 of Arrestin3.
    Depositor
    Louis Luttrell
    Publication
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Arrestin3
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Available since
    April 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  17. pCDNA3-SLPI

    Plasmid
    #18103
    Depositor
    Ronny Drapkin
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Secretory Leucocyte Protease Inhibitor (SLPI Human)
    Expression
    Mammalian
    Available since
    June 11, 2008
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  20. XBB.1.5+L455F+F456L

    Plasmid
    #208588
    Purpose
    Mammalian expression vector for expressing XBB.1.5+L455F+F456L SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation
    Depositor
    Ben Murrell
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 variant XBB.1.5+L455F+F456L spike (S )
    Expression
    Mammalian
    Available since
    Oct. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    208588-plasmid-map-sequence-id-409581

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Showing: 4181 - 4200 of 7094 results