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OSCA1.2-K435L-peGFP-N1

Plasmid

#228586

Purpose

Expresses OSCA1.2-K435L-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)

Expression

Mammalian

Mutation

K435L

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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OSCA1.2-A439K-peGFP-N1

Plasmid

#228589

Purpose

Expresses OSCA1.2-A439K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)

Expression

Mammalian

Mutation

A439K

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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RcSxph

Plasmid

#194018

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Tags

His10 and mEGFP,

Promoter

Polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-UBB

Plasmid

#222569

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of UBB

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

UBB (UBB Human)

Expression

Mammalian

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO FLAG-RPS28

Plasmid

#203156

Purpose

N-FLAG-RPS28 in inducible pcDNA5 vector for Flp-In integration

Depositor

Susan Baserga

Article

Bryant et al Nucleic Acids Res. 2024 Feb 28;52(4):198

Insert

RPS28 (RPS28 Human)

Tags

1X FLAG tag with GGGGS linker

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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RGG-BcLOV-mCh-SOS

Plasmid

#208278

Purpose

Expresses RGG-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

RGG-BcLOV-mCh-SOS

Use

Lentiviral

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Fus-BcLOV-mCh-EGFR

Plasmid

#208281

Purpose

Expresses Fus-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

Fus-BcLOV-mCh-EGFR

Use

Lentiviral

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET3a-UBE2G2

Plasmid

#204209

Purpose

Bacterial expression of UBE2G2

Depositor

Allan Weissman

Article

Das et al Mol Cell. 2009 Jun 26. 34(6):674-85.

Insert

UBE2G2 (Ube2g2 Mouse)

Expression

Bacterial

Mutation

none

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHWBF09exp

Plasmid

#130833

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP1

Tags

eGFP-FLAG-HA

Expression

Plant

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKP2del4

Plasmid

#130837

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP2

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of terminal four amino acid residues

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKP1delTM

Plasmid

#130841

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP1

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of transmembrane domain

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKG1delKASH

Plasmid

#130842

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKG1

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of KASH domain

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKS2exp

Plasmid

#130856

Purpose

Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

Depositor

Hank Bass

Article

Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

Insert

MLKS2

Tags

eGFP-FLAG-HA

Expression

Plant

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pHWBF07exp

Plasmid

#130832

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP2

Tags

eGFP-FLAG-HA

Expression

Plant

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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ARP3-V5-TurboID

Plasmid

#195144

Purpose

measure COR1C interaction boichemically

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

ACTR3 (ACTR3 Human)

Expression

Mammalian

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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COR1C CC-Halo

Plasmid

#195146

Purpose

image COR1C

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Expression

Mammalian

Mutation

truncated protein

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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Dcp2 H237A-EGFP

Plasmid

#201425

Purpose

Bacterial protein expression of Dcp2 H237A

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

H237A

Promoter

tac

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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Dhh1 25-425-EGFP

Plasmid

#201430

Purpose

Bacterial protein expression of Dhh1 25-425

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dhh1 (DHH1 Budding Yeast)

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 25-425 only

Promoter

tac

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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Dcp2 301-970-EGFP

Plasmid

#201429

Purpose

Bacterial protein expression of Dcp2 301-970

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 301-970 only

Promoter

tac

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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Dhh1 426-506-EGFP

Plasmid

#201431

Purpose

Bacterial protein expression of Dhh1 426-506

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dhh1 (DHH1 Budding Yeast)

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Mutation

aa 426-506 only

Promoter

tac

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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