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CROP_C9_Puro_FGFR1

Plasmid

#183289

Purpose

All-in-One CRISPRko system with a guide RNA that targets FGFR1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FGFR1

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_FGFR2

Plasmid

#183290

Purpose

All-in-One CRISPRko system with a guide RNA that targets FGFR2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FGFR2

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-3xmRuby-N

Plasmid

#113754

Purpose

Gateway multi-site entry clone for second position 3x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2-mRuby2-mRuby2

Use

CRISPR; Gateway entry vector

Tags

3xmRuby2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_EGFR

Plasmid

#183285

Purpose

All-in-One CRISPRko system with a guide RNA that targets EGFR gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

EGFR

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_SRC

Plasmid

#183322

Purpose

All-in-One CRISPRko system with a guide RNA that targets SRC gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

SRC

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_ABL2

Plasmid

#183268

Purpose

All-in-One CRISPRko system with a guide RNA that targets ABL2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ABL2

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_ABL1

Plasmid

#183267

Purpose

All-in-One CRISPRko system with a guide RNA that targets ABL1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ABL1

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBS/KS-mId4sp

Plasmid

#16046

Depositor

Robert Benezra

Article

Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

Insert

Id4 (Id4 Mouse)

Expression

Bacterial

Mutation

cDNA nucleotides 665-945

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pBbdCas9S_P0526-sgRNA500

Plasmid

#149641

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)

Plasmid

#149574

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)

Plasmid

#149581

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-FRNK

Plasmid

#74481

Purpose

This plasmid was constructed in order to characterize the function of Proline-rich tyrosine kinase 2 (PYK2).

Depositor

J Thomas Parsons

Article

Xiong et al J Cell Biol. 1997 Oct 20;139(2):529-39.

Insert

FRNK (PTK2 Chicken)

Tags

c-myc

Expression

Mammalian

Mutation

FRNK is the C-terminal, noncatalytic domain of th…

Promoter

CMV

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only
CROP_C9_Puro_ESR1

Plasmid

#183288

Purpose

All-in-One CRISPRko system with a guide RNA that targets ESR1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ESR1

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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TA0474_pET21a(+)

Plasmid

#13839

Depositor

SeWon Suh

Article

Kang et al Acta Crystallograph Sect F Struct Biol C

Insert

IS200 transposase (Ta0474 Thermoplasma acidophilum)

Expression

Bacterial

Mutation

See Depositor Comments Below

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

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pDisplay FLIPE-100u

Plasmid

#13547

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E S118W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

S118W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNAflaB

Plasmid

#149642

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNAmreB

Plasmid

#149653

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc Caronte (CT#612)

Plasmid

#13861

Depositor

Cliff Tabin

Article

Yokouchi et al Cell. 1999 Sep 3. 98(5):573-83.

Insert

Caronte (CER1 Chicken)

Tags

myc

Expression

Mammalian

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pFLAG Caronte (CT#304)

Plasmid

#13860

Depositor

Cliff Tabin

Article

Yokouchi et al Cell. 1999 Sep 3. 98(5):573-83.

Insert

Caronte (CER1 Chicken)

Tags

flag

Expression

Mammalian

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_arr2

Plasmid

#149639

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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