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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,313 results for: pme

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Showing: 4241 - 4260 of 4313 results

  4. pSW002-Pc-mRuby2

    Plasmid
    #111250
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mRuby2 (codon optimized for P. fluorescens)
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    mRuby2
    Expression
    Bacterial
    Mutation
    mRuby2 is codon optimized for expression in P. fl…
    Promoter
    Pc
    Available Since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111250-plasmid-map-sequence-id-215255

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  6. pCAS9c

    Plasmid
    #141250
    Purpose
    Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    TEF1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141250-plasmid-map-sequence-id-308875

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  7. pCAS9id

    Plasmid
    #141251
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141251-plasmid-map-sequence-id-308879

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  8. pCAS9cd

    Plasmid
    #141252
    Purpose
    Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    TEF1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141252-plasmid-map-sequence-id-308887

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  11. pMM0646

    Plasmid
    #129007
    Purpose
    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    KanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    Expression
    Yeast
    Available Since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129007-plasmid-map-sequence-id-259441

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  12. pVG122

    Plasmid
    #211765
    Purpose
    Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:
    Insert
    strongLOV
    Use
    For single-copy integration (if cut with pmei)
    Tags
    SV40 NLS and VP16
    Expression
    Yeast
    Mutation
    compared to EL222, strongLOV contains Glu84Asp mu…
    Available Since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211765-plasmid-map-sequence-id-417945

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  13. pMM0638

    Plasmid
    #129005
    Purpose
    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
    Expression
    Yeast
    Available Since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    129005-plasmid-map-sequence-id-261246

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  18. pLpC-Reg-L-IFP-F[2]+Venus (mammalian)

    Plasmid
    #52903
    Purpose
    Binary retroviral pl. (puromycin). Reg:Linker (GGGGS)X2+IFP-F[2] (PacI/EcoRI). In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Article
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    Reg-L-IFP-F[2]+Venus
    Use
    Retroviral
    Tags
    L-IFP-F[2]
    Expression
    Mammalian
    Promoter
    Unknown
    Available Since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pLpC-Cat-L-IFP-F[1]+Plum (mammalian)

    Plasmid
    #52905
    Purpose
    Binary retroviral pl. (puromycin). Cat(PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Article
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    Cat-L-IFP-F[1]+Plum
    Use
    Retroviral
    Tags
    L-IFP-F[1]
    Expression
    Mammalian
    Promoter
    Unknown
    Available Since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pLpC-Zipp.-L-IFP-F[2]+Venus (mammalian)

    Plasmid
    #52906
    Purpose
    Binary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Article
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    Zipp.-L-IFP-F[2]+Venus
    Use
    Retroviral
    Tags
    L-IFP-F[2]
    Expression
    Mammalian
    Promoter
    Unknown
    Available Since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 4241 - 4260 of 4313 results