We narrowed to 8,227 results for: sars

1. pcDNA3 Step-FLAG FBXO21

   Plasmid

   #236458

   Purpose

   transient overexpression of FBXO21 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Donato et al Nat Cell Biol. 2017 Apr;19(4):341-351. d

   Insert

   FBXO21 (FBXO21 Human)
   

   Use

   Tags

   Strep-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3 Strep-FLAG KBP

   Plasmid

   #236459

   Purpose

   transient overexpression of KBP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Donato et al Nat Cell Biol. 2017 Apr;19(4):341-351. d

   Insert

   KBP (KIF1BP Human)
   

   Use

   Tags

   Strep-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA4/TO-ORF66 C423A-2xStrep

   Plasmid

   #131111

   Purpose

   Expresses KSHV ORF66 a.a. 1-429 (full length) C423A mutant with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

   Insert

   ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
   

   Use

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   ORF66 a.a. 1-429 (full-length); cysteine 423 muta…

   Promoter

   CMV

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA4/TO-ORF66 C399A-2xStrep

   Plasmid

   #131112

   Purpose

   Expresses KSHV ORF66 a.a. 1-429 (full length) C399A mutant with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

   Insert

   ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
   

   Use

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   ORF66 a.a. 1-429 (full-length); cysteine 399 muta…

   Promoter

   CMV

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA4/TO-ORF66 C393A-2xStrep

   Plasmid

   #131113

   Purpose

   Expresses KSHV ORF66 a.a. 1-429 (full length) C393A mutant with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

   Insert

   ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
   

   Use

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   ORF66 a.a. 1-429 (full-length); cysteine 393 muta…

   Promoter

   CMV

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA4/TO-ORF66 C355A-2xStrep

   Plasmid

   #131114

   Purpose

   Expresses KSHV ORF66 a.a. 1-429 (full length) C355A mutant with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

   Insert

   ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
   

   Use

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   ORF66 a.a. 1-429 (full-length); cysteine 355 muta…

   Promoter

   CMV

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA4/TO-ORF66 C344A-2xStrep

   Plasmid

   #131115

   Purpose

   Expresses KSHV ORF66 a.a. 1-429 (full length) C344A mutant with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

   Insert

   ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
   

   Use

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   ORF66 a.a. 1-429 (full-length); cysteine 344 muta…

   Promoter

   CMV

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA4/TO-ORF66 C341A-2xStrep

   Plasmid

   #131116

   Purpose

   Expresses KSHV ORF66 a.a. 1-429 (full length) C341A mutant with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

   Insert

   ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
   

   Use

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   ORF66 a.a. 1-429 (full-length); cysteine 341 muta…

   Promoter

   CMV

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA4/TO-ORF66 C301A-2xStrep

   Plasmid

   #131117

   Purpose

   Expresses KSHV ORF66 a.a. 1-429 (full length) C301A mutant with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

   Insert

   ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
   

   Use

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   ORF66 a.a. 1-429 (full-length); cysteine 301 muta…

   Promoter

   CMV

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA4/TO-ORF66 C298A-2xStrep

    Plasmid

    #131118

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C298A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 298 muta…

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA4/TO-ORF66 C295A-2xStrep

    Plasmid

    #131119

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C295A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 295 muta…

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA4/TO-ORF66 C289A-2xStrep

    Plasmid

    #131120

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C289A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 289 muta…

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA4/TO-ORF66 C244A-2xStrep

    Plasmid

    #131121

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C244A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 244 muta…

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA4/TO-ORF66 200-429-2xStrep

    Plasmid

    #130955

    Purpose

    Expresses KSHV ORF66 a.a. 200-429 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 200-429

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA4/TO-ORF66 C427A-2xStrep

    Plasmid

    #131109

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C427A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 427 muta…

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA4/TO-ORF66 C424A-2xStrep

    Plasmid

    #131110

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C424A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 424 muta…

    Promoter

    CMV

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid

    #182287

    Purpose

    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    

    Use

    Tags

    nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Y306A/Y384F (AF) double mutant of Methanosarcina …

    Promoter

    CMV and U6

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRaGE Pyl TAG GFP Y35TAG

    Plasmid

    #160041

    Purpose

    Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.

    Depositor

    Lital Alfonta

    Article

    Ozer et al Front Bioeng Biotechnol. 2021 Feb 26;9:5

    Inserts

    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    deGFP
    

    Use

    Synthetic Biology

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Changed tyrosine 35 to TAG stop-codon (Site numbe…

    Promoter

    P70b promoter and T500 terminator, Vibrio natrieg…

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PX458-3xHA-en31FnCas9ABEmax8.17d

    Plasmid

    #201956

    Purpose

    Mammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    bpNLS-en31FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PX458-3xHA-dFnCas9

    Plasmid

    #201954

    Purpose

    Mammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-dFnCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D11A and H969A on FnCas9

    Promoter

    Cbh

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge