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pDONR223-MAPK13

Plasmid

#23739

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK13 (MAPK13 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-MAP2K5

Plasmid

#23805

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP2K5 (MAP2K5 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAP3K6

Plasmid

#23853

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K6 (MAP3K6 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCS2+ Beta Catenin (Xenopus)

Plasmid

#13434

Depositor

Marc Kirschner

Publication

Lee et al J Cell Biol. 2001 Sep 3. 154(5):983-93.

Insert

Beta Catenin (ctnnb1.L Frog)

Expression

Mammalian

Available since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pFlp-Km

Plasmid

#222334

Purpose

Expresses Flp and kanamycin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3

Depositor

Philip Poole

Publication

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

sacB (sacB B. subtilis)
flp (flp Budding Yeast)
cI (cI )

Promoter

cl, flp, and sacB

Available since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pRham-CALM1

Plasmid

#182500

Purpose

Bacterial expression of the Homo sapiens calmodulin, CALM1

Depositor

Dan Mulvihill

Publication

Baker et al FEBS Lett. 2022 Jan 31. doi: 10.1002/187

Insert

CALM1 (CALM1 Human)

Expression

Bacterial

Promoter

rhamnose

Available since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFEndoNA2

Plasmid

#174667

Purpose

Expresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

Depositor

Jukka Finne

Publication

Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

Insert

GFP-endoNA2 fusion protein

Tags

Histag

Expression

Bacterial

Promoter

T5

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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ETV2_pet28a

Plasmid

#131665

Purpose

Bacterial expression of ETS gene ETV2

Depositor

Peter Hollenhorst

Publication

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV2 (ETV2 Human)

Tags

6xHis

Expression

Bacterial

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRC11-U6-DR-crRNA-BsmbI(x2); EFS-Puro-WPRE

Plasmid

#123360

Purpose

Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat, and constitutive expression of puromycin resistance.

Depositor

Sidi Chen

Publication

Chow et al Nat Methods. 2019 May;16(5):405-408. doi

Insert

Empty LbCpf1 crRNA cloning cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOBCol2.3-GFPemd

Plasmid

#110210

Purpose

Labels cells in the late osteoblast lineage.

Depositor

David Rowe

Publication

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Insert

eGFPemerald

Tags

None

Expression

Mammalian

Promoter

2.3 kb rat type 1 collagen + first intron

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSET-pr-mEos3.2 (mEosFP3.2-A69T)

Plasmid

#99224

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).

Depositor

Periklis Pantazis

Publication

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos3.2

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::HygroR-bGHpA

Plasmid

#62436

Purpose

MXS_chaining vector with PGK::HygroR-bGHpA

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against hygromycin B with PGK Promoter

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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mEos2-Golgi-7

Plasmid

#57382

Purpose

Localization: Golgi Complex, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Publication

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Golgi (B4GALT1 Human)

Tags

mEos2

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pME-puro-CLIP-FLAG-CD59

Plasmid

#50375

Purpose

Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

Depositor

Reika Watanabe

Publication

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

CLIP

Expression

Mammalian

Promoter

SR alpha

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAG247

Plasmid

#33200

Depositor

David Bartel

Publication

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

LRIG1 (LRIG1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

SV40 early enhancer/promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223-RPS6KC1

Plasmid

#23460

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RPS6KC1 (RPS6KC1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pInducer-YAP1-S127A

Plasmid

#213584

Purpose

Doxycycline inducible expression of YAP1-S127A cDNA in mammalian cells

Depositor

Channing Der

Publication

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

S127A

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-p62-Silent_Mutation_WT

Plasmid

#187986

Purpose

human p62 with silent mutation for sip62 #5 (Thermo Scientific). Internal reference: SMC516

Depositor

Sascha Martens

Publication

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Tags

mCherry

Expression

Mammalian

Mutation

silent mutation for resistance to sip62 #5

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1_ 2xFLAG-2xSTREP_FBXO22

Plasmid

#159127

Purpose

expresses FBXO22 in mammalian cells

Depositor

Michele Pagano

Publication

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

FBXO22 (FBXO22 Human)

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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IZ501: pMVP/Lenti-DEST

Plasmid

#121848

Purpose

pMVP destination vector, empty Lentivirus vector backbone

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Lentiviral; Pmvp gateway destination vector

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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