Addgene

We narrowed to 7,257 results for: trac

Showing: 4241 - 4260 of 7257 results

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pHBJT020

Plasmid

#225171

Purpose

Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (TetR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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N-SF-TAP-DEST-TLK2-D592V

Plasmid

#218835

Purpose

Expresses N-terminal Strep-FLAG-tagged human TLK2 mutant D592V (kinase dead).

Depositor

Travis Stracker

Insert

TLK2 Q592V (kinase dead) (TLK2 Human)

Use

Tags

FLAG-STREP

Expression

Mammalian

Mutation

Q592V

Promoter

CMV

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBH504

Plasmid

#215782

Purpose

NBU1 backbone with constitutive dCas9, LacI(YQR), P(O1)_sgRNA targeting B. theta amylopectin SusC gene

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(YQR)
sgRNA-ribozyme cassette
dCas9

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Bt1311, P(O1), and P1

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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P-BT2818

Plasmid

#195742

Purpose

pBolux harboring the promoter region upstream of BT2818

Depositor

Guy Townsend

Article

Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

Insert

The B. thetaiotaomicron BT2818 promoter cloned into pBolux

Use

Tags

Expression

Bacterial

Mutation

Promoter

BT2818

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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P-BACOVA_04505

Plasmid

#188112

Purpose

pBolux harboring the promoter region upstream of BACOVA_04505

Depositor

Guy Townsend

Article

Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

Insert

The B. ovatus BACOVA_04505 promoter cloned into pBolux

Use

Tags

Expression

Bacterial

Mutation

Promoter

BACOVA_04505

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-TC

Plasmid

#67169

Purpose

CRISPaint universal donor for tetracysteine fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

TC Tag

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pET His10-Draper INT

Plasmid

#126670

Purpose

Expression of His10-Draper intracellular domain for purification from E. coli

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper cytoplasmic domain

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pRH030

Plasmid

#159633

Purpose

CRISPR‐Cas9 tool for the genetic engineering of Bacillus phages

Depositor

Robert Hertel

Article

Otte et al Lett Appl Microbiol. 2020 Jul 2. doi: 10

Type

Empty backbone

Use

CRISPR; Shuttle vector

Tags

Expression

Mutation

Promoter

Constitutive tracrRNA

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pTKLP-tetA

Plasmid

#71325

Purpose

tetA landing pad template plasmid

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMBIS

Plasmid

#17817

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

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pYPQ132-tRNA

Plasmid

#179211

Purpose

Golden Gate entry vector; 2nd gRNA with tRNA

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

no promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCM271rfp

Plasmid

#59774

Purpose

pCMrfp with TrfA mutation (271R to C)

Depositor

Nathan Hillson

Article

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

RFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ134B

Plasmid

#179216

Purpose

Golden Gate entry vector; 4th gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

AtU3

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-FRT

Plasmid

#183241

Purpose

anhydrotetracycline (aTC) inducible sgRNA that targets FRT scar sites and arabinose inducible lambda Red

Depositor

Karin Schnetz

Article

Rangarajan et al Microbiology (Reading). 2022 Apr;168(4).

Insert

sgRNA-FRT

Use

Tags

Expression

Bacterial

Mutation

Promoter

P-tet

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-CalfluxVTN

Plasmid

#83926

Purpose

Bioluminescent Reporter of Intracellular Calcium

Depositor

Carl Johnson

Article

Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

Insert

CalfluxVTN

Use

Tags

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pSLTS

Plasmid

#59386

Purpose

Expresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.

Depositor

Shelley Copley

Article

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Type

Empty backbone

Use

Genome engineering

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pJLG039

Plasmid

#192982

Purpose

BHR plasmid (IncP ori) expressing T7 polymerase under control of the lac promoter

Depositor

Clay Fuqua

Article

Greenwich et al Microbiol Resour Announc. 2023 Feb 16;12

Insert

T7 polymerase

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAX1

Plasmid

#117397

Purpose

allelic exchange vector with GFP merodiploid tracker and temperature-sensitive origin of replication

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pBBR2k-GFPuv

Plasmid

#106383

Purpose

Anhydrotetracycline-inducible tet promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Use

Tags

Expression

Bacterial

Mutation

Promoter

tet

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI

Plasmid

#32061

Purpose

Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

Depositor

Miguel Valvano

Article

Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

Insert

I-SceI nuclease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pdhfr

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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