We narrowed to 9,297 results for: yeast expression

1. pAW240

   Plasmid

   #170791

   Purpose

   P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution

   Depositor

   Chang Liu

   Article

   Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

   Insert

   p10B2-AT110
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   10B2 (P1 specific promoter)

   Available Since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAW212

   Plasmid

   #172196

   Purpose

   P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites

   Depositor

   Chang Liu

   Article

   Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

   Insert

   p10B2-AT110
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   10B2 (P1 specific promoter)

   Available Since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8

   Plasmid

   #207031

   Purpose

   Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Pho8
   

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG416GAL-ccdB-mRuby3

   Plasmid

   #166139

   Purpose

   Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a CEN/ARS element for low copy number when in yeast.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Type

   Empty backbone

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. mito-yHS1-M7A,H102A

   Plasmid

   #159165

   Purpose

   Mitochondrial labile heme reporter

   Depositor

   Amit Reddi

   Article

   Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

   Insert

   mito-yHS1-M7A,H102A
   

   Use

   Tags

   COX4 pre-sequence

   Expression

   Yeast

   Mutation

   Mutated both Met 7 and His 102 of the cyt b562 mo…

   Promoter

   GPD

   Available Since

   Sept. 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAG426GAL-ccdB-mRuby3

   Plasmid

   #166142

   Purpose

   Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a 2um element for high copy number when in yeast.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Type

   Empty backbone

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pROS_phleo-Tps1/Gsy2

   Plasmid

   #196612

   Purpose

   Encoding guide RNAs for the knock out of TPS1 and GSY2 genes

   Depositor

   Matthias Heinemann

   Article

   Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

   Insert

   TPS1, GSY2 (TPS1, GSY2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available Since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIFEX-A2aGH

   Plasmid

   #160542

   Purpose

   pITY4 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   N/A

   Promoter

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEFEX1-A2aGH

   Plasmid

   #160543

   Purpose

   pYES2 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   N/A

   Promoter

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCFEX1-A2aGH

    Plasmid

    #160546

    Purpose

    pYC2/CT derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N/A

    Promoter

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. p414ADH_Hsp82_CycTER

    Plasmid

    #61712

    Purpose

    yeast plasmid with Hsp82

    Depositor

    Dan Bolon

    Article

    Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

    Insert

    6xHis-Hsp82 (HSP82 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Spt2-GFP

    Plasmid

    #115572

    Purpose

    Expresses yeast Spt2-GFP fusion protein

    Depositor

    Michael Downey

    Article

    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

    Insert

    SPT2
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. p414TEF_Hsp82_CycTER

    Plasmid

    #61711

    Purpose

    Yeast plasmid with Hsp82

    Depositor

    Dan Bolon

    Article

    Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

    Insert

    6xHis-Hsp82 (HSP82 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. p414GPD_Hsp82_CycTER

    Plasmid

    #61710

    Purpose

    Yeast plasmid with Hsp82

    Depositor

    Dan Bolon

    Article

    Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

    Insert

    6xHis-Hsp82 (HSP82 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. p414CYC_Hsp82_CycTER

    Plasmid

    #61713

    Purpose

    yeast plasmid with Hsp82

    Depositor

    Dan Bolon

    Article

    Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

    Insert

    6xHis-Hsp82 (HSP82 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    CYC1

    Available Since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. p414TEF_Hsp82_noTER

    Plasmid

    #61714

    Purpose

    yeast plasmid with Hsp82

    Depositor

    Dan Bolon

    Article

    Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

    Insert

    6xHis-Hsp82 (HSP82 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p414ADH_Hsp82_noTER

    Plasmid

    #61715

    Purpose

    yeast plasmid with Hsp82

    Depositor

    Dan Bolon

    Article

    Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

    Insert

    6xHis-Hsp82 (HSP82 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p414CYC_Hsp82_noTER

    Plasmid

    #61716

    Purpose

    yeast plasmid with Hsp82

    Depositor

    Dan Bolon

    Article

    Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

    Insert

    6xHis-Hsp82 (HSP82 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    CYC1

    Available Since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. YIPlac204TKC-DsRed-Express2-HDEL

    Plasmid

    #21770

    Depositor

    Benjamin Glick

    Insert

    DsRed-Express2
    

    Use

    Tags

    HDEL and preKar2

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAS

    Plasmid

    #60847

    Purpose

    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

    Depositor

    Jamie Cate

    Article

    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

    Inserts

    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    NLS/His8 and TTT 3' extension prior to sgRNA

    Expression

    Bacterial and Yeast

    Mutation

    L4 is UUCG tetraloop and guide targets LYP1 (CATA…

    Promoter

    Available Since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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