We narrowed to 2,346 results for: puc19

1. pAB31 (punc-47::GtACR2::mCerulean::bHK::Chrimson)

   Plasmid

   #214891

   Purpose

   Expresses the optogenetic tool BiPOLES in GABAergic neurons of C. elegans.

   Depositor

   Alexander Gottschalk

   Article

   Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

   Insert

   GtACR2_mCerulean_bHK_Chrimson
   

   Use

   Tags

   mCerulean

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. PE-pegRNA(BG)-nsgRNA(BG)

   Plasmid

   #232727

   Purpose

   A dual-targeting vector that contains PE3 system guide RNAs against BFP (pegRNA(BG) and nsgRNA(BG)) with restriction enzyme sites for insertion of target site guide RNAS (pegRNAs(TS) and nsgRNA(TS)).

   Depositor

   David Brafman

   Article

   Frisch et al Mol Ther Nucleic Acids. 2023 Jul 17;33:4

   Insert

   pegRNA(BG), nsgRNA(BG), pegRNAs(TS), nsgRNA(TS)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-N-Solo-mStayGold-LMNB1

   Plasmid

   #227328

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a mStayGold Tag (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-C-Solo-mScarlet-MAPRE1

   Plasmid

   #227323

   Purpose

   Donor template for mScarlet insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   MAPRE1 Homology Arms flanking a mScarlet Tag (MAPRE1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-KO-Puro-TP53

   Plasmid

   #227319

   Purpose

   Donor template for 2A-Puro insertion into the N-terminus of the TP53 locus. For selectable p53 knock-out. To be co-transfected with sgRNA plasmid px330-TP53 (Addgene #227318)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TP53 Homology Arms flanking a 2A-Puro Cassette (TP53 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-Solo-mStayGold-EZR

   Plasmid

   #227294

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the EZR locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-EZR (Addgene #227293)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   EZR Homology Arms flanking a mStayGold Tag (EZR Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-C-mStayGold-EFS-Blast-ARL13B

   Plasmid

   #227278

   Purpose

   Donor template for mStayGold-EFS-Blast insertion into the C-terminus of the ARL13B locus. For cilia visualization. To be co-transfected with plasmid pX330-PITCh-ARL13B (Addgene #227276)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ARL13B Homology Arms flanking a mStayGold-EFS-Blast Cassette (ARL13B Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-pCAG-mock

   Plasmid

   #228504

   Purpose

   This plasmid can be used for control experiments and can also be used as a plasmid backbone

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-protein-mock

   Plasmid

   #228505

   Purpose

   This plasmid can be used for control experiments and can also be used as a plasmid backbone

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-Solo-mStayGold-H3C2

    Plasmid

    #227334

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H3C2 Homology Arms flanking a mStayGold Tag (H3C2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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