We narrowed to 23,628 results for: crispr

1. pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18

   Plasmid

   #172316

   Purpose

   CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJZC588

   Plasmid

   #62315

   Purpose

   sgRNA with 2x MS2 (wt+f6) for yeast cells

   Depositor

   Stanley Qi, Wendell Lim

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   sgRNA + 2x MS2 (wt+f6) binding module
   

   Expression

   Yeast

   Promoter

   SNR52

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC91-pmax-dCas9VP64

   Plasmid

   #48223

   Purpose

   dCas9VP64 on pmax expression vector

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP64 activation domain
   

   Use

   CRISPR

   Tags

   HA Tag and VP64

   Expression

   Mammalian

   Mutation

   D10A;H840A (catalytically inactive)

   Promoter

   CAGGS

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX458_AHR_1

   Plasmid

   #101076

   Purpose

   Encodes gRNA for 3' target of human AHR

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against AHR (AHR Human)
   

   Use

   CRISPR

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY014 (pcDNA3.1-hMbCpf1)

   Plasmid

   #69986

   Purpose

   Expresses humanized MbCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hMbCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEJS468-pLK.O1-NmeSgRNA/DTS13-Telomere

   Plasmid

   #85714

   Purpose

   Telomere-targeting Nme sgRNA under U6 promoter

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Insert

   telomere sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP977

   Plasmid

   #65774

   Purpose

   Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135E mutation in Cas9

   Promoter

   CMV & T7

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX458_KDM1A_1

   Plasmid

   #86297

   Purpose

   Encodes gRNA for 3' target of human KDM1A

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against KDM1A (KDM1A Human)
   

   Use

   CRISPR

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMpGE006

   Plasmid

   #108722

   Purpose

   Cas9 expression in M.polymorpha

   Depositor

   Takayuki Kohchi, Shigeo Sugano

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Insert

   Atco-Cas9-Pea3ter
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   MpEFpro

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX458_KDM1A_2

    Plasmid

    #86298

    Purpose

    Encodes gRNA for 3' target of human KDM1A

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KDM1A (KDM1A Human)
    

    Use

    CRISPR

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC029 - Lw2Cas13a from Leptotrichia wadei F0279

    Plasmid

    #91919

    Purpose

    Expresses Lw2Cas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    Lw2Cas13a and guide expression cassette
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-SMVP-Cas9N-U6-gRNA P2

    Plasmid

    #80944

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA P2 for Pd-l1 binding

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT3TS-zAsCpf1

    Plasmid

    #107185

    Purpose

    plasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)

    Depositor

    Antonio Giraldez

    Article

    Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

    Insert

    AsCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBluescriptSKII+ U6-sgRNA(F+E) TFRC

    Plasmid

    #74709

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNA

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pSP64T-hLbCpf1

    Plasmid

    #107187

    Purpose

    plasmid to carry out IVT of human codon optimized LbCpf1 (based on Addgene 69988)

    Depositor

    Antonio Giraldez

    Article

    Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

    Insert

    hLbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Promoter

    sp6

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. BPK2797

    Plasmid

    #72251

    Purpose

    Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335Q, and T1337R

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfB3045(gRNA XI-3)

    Plasmid

    #73287

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-3

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. c3GIC9n

    Plasmid

    #62192

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9 (D10A)
    

    Use

    CRISPR

    Tags

    HA

    Mutation

    D10A substitution (Cas9n)

    Promoter

    TRE3G

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pY018 (pcDNA3.1-hPdCpf1)

    Plasmid

    #69990

    Purpose

    Expresses humanized PdCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hPdCpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX458_AHR_2

    Plasmid

    #101077

    Purpose

    Encodes gRNA for 3' target of human AHR

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against AHR (AHR Human)
    

    Use

    CRISPR

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge