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Plasmid#69547PurposeDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.DepositorInsertsUseMouse TargetingTags2x nuclear localization signal and PVT1-2A "…MutationSilent V205V mutation in myogenin gene to prevent…Available SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only
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pLenti-hSyn-Gαi*-BERKY1
Plasmid#158427PurposeBRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-KB1753-myc
UseLentiviralTagsmycExpressionMammalianPromoterhSynapsinAvailable SinceSept. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pU6-Sp-gRNA-IDS_DF_C2c_attB_rev
Plasmid#182151PurposeTo insert attB-rev attachment site at IDS in human cells via twinPEDepositorInsertIDS-C2c-attBrev pegRNA
ExpressionMammalianPromoterhU6Available SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-2 (C50 29 nM)
Plasmid#117909PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)
TagsHis-tag and Strep-tagExpressionBacterialMutationR98A on eDHFRPromoterT7Available SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-BbsI-Btl
Plasmid#244934PurposeCRISPR gRNA targeting the C-terminal end of Breathless for cleavage.DepositorInsertgRNA targeting Btl C terminus
UseCRISPRAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
mPLD2-PX (62-192)
Plasmid#119128PurposeBacterial expression of human phox homology (PX) domain, mPLD2-PX (62-192)DepositorInsertmPLD2-PX (62-192)
TagsGSTExpressionBacterialPromotertacAvailable SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR_Trf2
Plasmid#125159PurposeGateway entry cloneDepositorInsertTrf2 (Trf2 Fly)
UseGateway CloningAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_Theo-LUCID
Plasmid#119048PurposePlasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_Theo-LUCID_part1
Fab_Theo-LUCID_part2
TagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_Quin-LUCID
Plasmid#119049PurposePlasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_Quin-LUCID_part1
Fab_Quin-LUCID_part2
TagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_MTX-LUCID
Plasmid#119047PurposePlasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_MTX-LUCID_part1
Fab_MTX-LUCID_part2
TagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
SNX26-PX (56-172)
Plasmid#119105PurposeBacterial expression of human phox homology (PX) domain, SNX26-PX (56-172)DepositorInsertSNX26-PX (56-172)
TagsGSTExpressionBacterialPromotertacAvailabilityAcademic Institutions and Nonprofits only -
SNX28-PX (1-124)
Plasmid#119107PurposeBacterial expression of human phox homology (PX) domain, SNX28-PX (1-124)DepositorInsertSNX28-PX (1-124)
TagsGSTExpressionBacterialPromotertacAvailabilityAcademic Institutions and Nonprofits only -
SNX34-PX (1-130)
Plasmid#119113PurposeBacterial expression of human phox homology (PX) domain, SNX34-PX (1-130)DepositorInsertSNX34-PX (1-130)
TagsGSTExpressionBacterialPromotertacAvailabilityAcademic Institutions and Nonprofits only -
IRAS-PX (13-124)
Plasmid#119118PurposeBacterial expression of human phox homology (PX) domain, IRAS-PX (13-124)DepositorInsertIRAS-PX (13-124)
TagsGSTExpressionBacterialPromotertacAvailabilityAcademic Institutions and Nonprofits only -
Actin Binding Domain Tension Sensor
Plasmid#215368PurposeExpresses Actin Binding Domain Tension Sensor (ABDTS), composed of Ftractin-Linker-TSMod-Linker-Ftractin. TSMod is the tension sensor module containing mTFP1 and mVenus.DepositorInsertActin Binding Domain Tension Sensor
ExpressionMammalianPromoterCMVAvailable SinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i1 ONE-GO
Plasmid#189735PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTol2CG2-QUAS5x:GFPNLS-SV40pA
Plasmid#155122PurposeTol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertQUAS5x:GFPNLS
UseZebrafish expressionPromoterQUAS5x-E1bAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-QUASM1:GFPNLS-SV40pA
Plasmid#155123PurposeTol2 vector containing QUASM1 reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertQUASM1:GFPNLS
UseZebrafish expressionPromoterQUASM1-E1bAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-q ONE-GO
Plasmid#189738PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3 Ash2L full
Plasmid#15548DepositorInsertAsh2L (ASH2L Human)
ExpressionMammalianMutationR622H (Please see depositor comments below)Available SinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only