We narrowed to 5,969 results for: actin

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pPB-MPP8-∆ARD_Flag2-Blast

Plasmid

#175041

Purpose

Mammalian expression of mMPP8 (deletion from D570 to K718) with C-terminal Flag2 tag

Depositor

Kristian Helin

Publication

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Mphosph8 (Mphosph8 Mouse)

Use

Piggybac

Tags

Flag2

Expression

Mammalian

Mutation

Expression of MPP8 protein with deletion of the a…

Promoter

CAG

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pF3-CAG-Tol-FRT

Plasmid

#141349

Purpose

Shuttling cassette containing the Tol ORF

Depositor

Ton Schumacher

Publication

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

Tol cassette

Use

Mouse Targeting

Tags

HA

Expression

Mammalian

Promoter

CAG

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-SRGAP3FBAR-EGFP

Plasmid

#168506

Purpose

Expression of F-BAR domain (aa 1-492) of SRGAP3 with a C-terminal EGFP fusion

Depositor

Franck Polleux

Publication

Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

Insert

SRGAP3-FBAR (SRGAP3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-492

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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RGG-mCherry-RGG-eDHFR

Plasmid

#164648

Purpose

Two RGG domains tagged with mCherry and eDHFR

Depositor

Michael Lampson, Huaiying Zhang

Publication

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

RGG

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-GFP-mirNega

Plasmid

#163705

Purpose

pAAV plasmid to induce expression of universal negative control micro-RNA and EGFP

Depositor

Michisuke Yuzaki

Publication

Ito-Ishida et al J Neurosci. 2020 Nov 4;40(45):8746-8766.

Insert

mirNega and EGFP

Use

AAV

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Venus-pA_U6-BbsI (pE057)

Plasmid

#149351

Purpose

Mammalian expression vector for Venus

Depositor

Ralf Kuehn

Publication

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

Venus

Expression

Mammalian

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-BFP-pA_U6-BbsI (pE058)

Plasmid

#149352

Purpose

Mammalian expression vector for BFP

Depositor

Ralf Kuehn

Publication

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

BFP

Expression

Mammalian

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-miRFP670-pA_U6-BbsI (pE059)

Plasmid

#149353

Purpose

Mammalian expression vector for miRFP670

Depositor

Ralf Kuehn

Publication

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

miRFP670

Expression

Mammalian

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NEO-CAG-BFP-pA-GFP-pA

Plasmid

#149344

Purpose

Vector for targeting the Replace reporter to the AAVS1 locus

Depositor

Ralf Kuehn

Publication

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

BFP

Expression

Mammalian

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pJM465 EF1α 3xFLAG-NLS-VP64-ZF1 in TUPV2

Plasmid

#161533

Purpose

Constitutive expression of 3xFLAG-NLS-VP64-ZF1 under the EF1α promoter

Depositor

Joshua Leonard

Publication

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP64-ZF1

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

EF1α

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAT-9222

Plasmid

#124222

Purpose

Cloning plasmid for EGxFP assay with self-targeting gRNA-s

Depositor

Ervin Welker

Publication

Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

Insert

self-targeting gRNA between EGFP halves

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS_2pabPAC

Plasmid

#158968

Purpose

2P-activatable bPAC for transient expression

Depositor

Michiyuki Matsuda

Publication

Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

Insert

2pabPAC

Expression

Mammalian

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-TetR-RNF169UBD

Plasmid

#119011

Purpose

Mammalian expression of a fusion protein of single chain Tet repressor with the human RNF169 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Publication

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

TetR

Expression

Mammalian

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pU6Rosa-CAG-Gal4-Rad18UBD

Plasmid

#119012

Purpose

Mammalian expression of Rosa26 sgRNA, fusion protein of yeast Gal4 with human Rad18 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Publication

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Gal4 (GAL4 Budding Yeast)

Expression

Mammalian

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pPB CAG Cdk6 R31C-IRES-EGFP

Plasmid

#153946

Purpose

piggyBac transposon vector with CAG promoter expressing Cdk6 (R31C mutant) and EGFP

Depositor

Luca Tiberi

Publication

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

Cdk6 (Cdk6 Mouse)

Expression

Mammalian

Mutation

R31C

Promoter

CAG

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCFL-mPML-2CAMut-iB

Plasmid

#140286

Purpose

Expression of mouse PML Ca mutant

Depositor

Yusuke Miyanari

Publication

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

PML (Pml Mouse)

Expression

Mammalian

Mutation

CA mutant (RING finger mutant)

Promoter

CAG

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)

Plasmid

#133790

Purpose

CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Publication

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized NmeCas9

Tags

NLS(SV40)-3xFLAG

Expression

Mammalian

Mutation

n/a

Promoter

CAG

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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U6_sgRNA_CAG_hSt1Cas9_MTH17CL396

Plasmid

#136656

Purpose

A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:MTH17CL396 chimera) and its U6-driven sgRNA.

Depositor

Yannick Doyon

Publication

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9:MTH17CL396 Chimera
sgRNA for St1Cas9

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG and hU6

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

Plasmid

#136659

Purpose

Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA

Depositor

Yannick Doyon

Publication

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD9:TH1477 chimera
sgRNA for St1Cas9
APOBEC-1 (APOBEC1 Human)
UGI
HA tag

Use

CRISPR and Synthetic Biology

Tags

2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

Expression

Mammalian

Promoter

CAG and U6

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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U6_sgRNA_CAG_hSt1Cas9_TH1477

Plasmid

#136655

Purpose

A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:TH1477 chimera) and its U6-driven sgRNA.

Depositor

Yannick Doyon

Publication

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9:TH1477 Chimera
sgRNA for St1Cas9

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG and hU6

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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