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JDW 1042 (pLenti6.2_CMV_V5_TCF7L1_P2A_H2A_mCherry)

Plasmid

#233540

Purpose

A CMV driven human TCF7L1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7L1 (TCF7L1 Human)

Use

Lentiviral

Tags

V5

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pGL198

Plasmid

#180472

Purpose

For expression of Cas9 and gRNAs, multiple gRNAs cloning vector

Depositor

Peggy Lemaux

Article

Aregawi et al Plant Biotechnol J. 2021 Nov 26. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

ZmCas9

Expression

Plant

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW COX8ASig-Lemon

Plasmid

#239560

Purpose

Expression and mitochondrial localization of Lemon, a pH biosensor

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

Lemon

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a(+)/FbGH30

Plasmid

#86463

Purpose

Expression of the Beta-1,6-Exo-Laminarinase FbGH30 in E.coli

Depositor

Jan-Hendrik Hehemann

Article

Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

Insert

FbGH30

Tags

His-Tag

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pFUW mCherry-P2A-NeoR

Plasmid

#239570

Purpose

Cytosolic expression of mCherry

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mCherry

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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GESTALT_pX330-v1

Plasmid

#103061

Purpose

plasmid px330 with guide targeting GESTALT v1 through V5 constructs

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

integration of Cas9 with guide targeting GESTALT barcodes V1 to V5

Use

Lentiviral

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-Rab9

Plasmid

#31736

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab9 (Rab9 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
GFP-TIS11B CPM2

Plasmid

#121163

Purpose

Mammalian expression of GFP tagged TIS11B CPM2 (charge pattern mutant 2)

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

ZFP36L1 (TIS11B) charge pattern mutant 2

Expression

Mammalian

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pKM263-ScPPX

Plasmid

#38327

Depositor

Florian Freimoser

Article

Werner et al Arch Microbiol. 2005 Nov;184(2):129-36.

Insert

PPX1 (PPX1 Budding Yeast)

Tags

GST, His, and TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pBA2kIKmA2

Plasmid

#39213

Depositor

Anastasios Melis

Article

Lindberg et al Metab Eng. 2010 Jan;12(1):70-9. Epub 200

Insert

Kudzu Isoprene Synthase

Use

Synthetic Biology

Mutation

Deleted the predicted chloroplast transit peptide

Promoter

psbA2

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
hOCT1-miRFP

Plasmid

#188995

Purpose

A mammalian expression plasmid encoding miRFP-tagged human OCT1

Depositor

Jeremy Baskin

Article

Chiu et al JACS Au. 2022 Nov 28;2(12):2703-2713. do

Insert

hOCT1 (SLC22A1 Human)

Tags

miRFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDest490-OPN-c

Plasmid

#17592

Depositor

Xin Wang

Article

Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Insert

OPN-c (SPP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Base pairs 1–93, 175–942

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pFUW mEmerald-P2A-PuroR

Plasmid

#239566

Purpose

Cytosolic expression of mEmerald

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mEmerald

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pQUASp-mCD8GFP

Plasmid

#46163

Purpose

Expression of murine CD8GFP in insects

Depositor

Christopher Potter

Article

Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

Insert

mCD8GFP

Expression

Insect

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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2 gRNA concatmer

Plasmid

#84879

Purpose

Empty concatmer vector in which 2 gRNAs can be inserted

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only
pGL193

Plasmid

#180471

Purpose

For expression of Cas9 and gRNAs, multiple gRNAs cloning vector

Depositor

Peggy Lemaux

Article

Aregawi et al Plant Biotechnol J. 2021 Nov 26. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Cas9

Expression

Plant

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 VP4-VP2

Plasmid

#216787

Purpose

Mammalian expression of the VP4-VP2 portion of the CVB3 Kandolf strain polyprotein

Depositor

Kevin Janes

Article

Griffiths et al Sci Adv. 2024 Nov 15;10(46):eadp6303. do

Insert

VP4-VP2

Expression

Mammalian

Promoter

CMV

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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Ai38

Plasmid

#34883

Depositor

Hongkui Zeng

Article

Zariwala et al J Neurosci. 2012 Feb 29;32(9):3131-41. d

Insert

CAG-Floxed GCaMP3

Use

Mouse Targeting

Promoter

CAG

Available Since

March 7, 2012

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-FKBP-HaloTag

Plasmid

#233350

Purpose

Stable expression of FKBP-Halotag in mammalian cells. FKBP-halotag localizes in the cytosol and binds to the FRB tag in the presence of AP21967 (A/C Heterodimerizer).

Depositor

Noboru Mizushima

Article

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Insert

FKBP

Use

Retroviral

Tags

HaloTag7

Expression

Mammalian

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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3 gRNA concatemer

Plasmid

#84880

Purpose

Empty concatmer vector in which 3 gRNAs can be inserted

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only