Addgene

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NK588

Plasmid

#109077

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

pActAvrII_nanoluc

Use

Choanoflagellate expression

Tags

Expression

Mutation

Eliminate NcoI site in the promoter

Promoter

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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NK606

Plasmid

#109076

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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NK587

Plasmid

#109075

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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NK583

Plasmid

#109072

Purpose

Parental vector for making S. rosetta expression vectors

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_10057

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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NK600

Plasmid

#109068

Purpose

Parental vector for making S. rosetta expression vectors

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_02256

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
TbSIR2RP1

Plasmid

#67242

Purpose

Determine its activity and specificity

Depositor

Cynthia Wolberger

Article

Fahie et al FEBS J. 2009 Dec;276(23):7159-76. doi: 1

Insert

TbSIR2RP1

Use

Tags

MBP, TEV, Thrombin, HIS, and none

Expression

Bacterial

Mutation

Promoter

p-lac

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only
pJK600

Plasmid

#71605

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-H59N,Y154F)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Use

Tags

Expression

Bacterial

Mutation

changes tryptophan-34 to phenylalanine, histidine…

Promoter

T7

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF1C10

Plasmid

#70841

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CG6907

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2E03

Plasmid

#70880

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CG8378

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2D08

Plasmid

#70912

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Ext2

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3H10

Plasmid

#71006

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

tlk

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only
pFOSGE_CUS1D02

Plasmid

#71039

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CG14222

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1G07

Plasmid

#70823

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Cp1

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1D01

Plasmid

#70776

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CG33785

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF4D05

Plasmid

#71033

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CG17446

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3D03

Plasmid

#70960

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

DNApol-delta

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_CUS1G07

Plasmid

#70953

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CG8481

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3D07

Plasmid

#70986

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

e(y)1

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2F07

Plasmid

#71010

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

pr-set7

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1G08

Plasmid

#71034

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CG5287

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only