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pLCKO-CMV-SARS-CoV-2-S-Bel-P5_5-7-IRES-mNeonGreen-NES/PKI-P2A-Blasticidin

Plasmid

#237480

Purpose

Encodes full-length SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate), featuring the E484D substitution, which likely emerged during passaging and enhances TMEM106B binding.

Depositor

Dirk Daelemans

Article

Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

Insert

SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate)

Use

Lentiviral

Tags

IRES-mNeonGreen-NES/PKI and P2A-Blasticidin

Expression

Mammalian

Mutation

S484D + S813I + deleted amino acids 68-76 + 676-6…

Promoter

CMV

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLCKO-CMV-TMEM106B-M210A_F213A-P2A-Blasticidin

Plasmid

#237479

Purpose

Encodes full-length human TMEM106B. M210A/F213A mutations block S1 binding and SARS-CoV-2 Belgium/GHB-03021/2020 TMEM106B mediated infection, without affecting TMEM106B expression or localization.

Depositor

Dirk Daelemans

Article

Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

Insert

TMEM106B (TMEM106B Human)

Use

Lentiviral

Tags

P2A-Blasticidin

Expression

Mammalian

Mutation

M210A + F213A

Promoter

CMV

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-eIF3B MBE3 mutant

Plasmid

#133310

Purpose

Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 3

Depositor

Josep Domingo-Domenech

Article

Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

Insert

eIF3B promoter MBE3 mutant (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Mutation

ccacgtgacc changed to cAaAAAAacc

Promoter

eIF3B

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4-eIF3B MBE2 mutant

Plasmid

#133309

Purpose

Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 2

Depositor

Josep Domingo-Domenech

Article

Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

Insert

eIF3B promoter MBE2 mutant (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Mutation

gccacatgcacc changed to gcAaAaAAcacc

Promoter

eIF3B

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

Plasmid

#202553

Purpose

Nontargeting control vector with TagBFP2

Depositor

Frederic Meunier

Article

Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

Insert

Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))

Use

CRISPR and Lentiviral

Tags

3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

Mutation

Puromycin-resistance gene in the pLV hU6-sgRNA hU…

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMOTag2T

Plasmid

#26295

Depositor

George Cross

Type

Empty backbone

Use

Trypanosome in-situ tagging vector

Tags

Ty-1

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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E. coli K-12 MG1655_rLP5(nth-ydgR)

Bacterial Strain

#110245

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the nth-ydgR intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
TB205 △trpC

Bacterial Strain

#230038

Purpose

Fluorescently labelled (mCherry) E. coli, auxotrophic for tryptophan

Depositor

Martin Ackermann, Olga Schubert

Article

Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

Bacterial Resistance

None

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △trpC

Bacterial Strain

#230037

Purpose

Fluorescently labelled (GFP) E. coli, auxotrophic for tryptophan

Depositor

Martin Ackermann, Olga Schubert

Article

Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

Bacterial Resistance

None

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_fLP4(essQ-cspB)

Bacterial Strain

#110244

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the essQ-cspB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_rLP6(ygcE-ygcF)

Bacterial Strain

#110246

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the ygcE-ygcF intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_rLP1(atpI-gidB)

Bacterial Strain

#110241

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the atpl-gidB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_fLP3(ybbD-ylbG)

Bacterial Strain

#110243

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the ybbD-ylbG intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_fLP2(yieN-trkD)

Bacterial Strain

#110242

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the yieN-trkD intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
pMOTag2H

Plasmid

#26296

Depositor

George Cross

Type

Empty backbone

Use

Trypanosome in-situ tagging vector

Tags

Triple HA

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pMOTag5H

Plasmid

#26299

Depositor

George Cross

Type

Empty backbone

Use

Trypanosome in-situ tagging vector

Tags

triple HA

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only
pMOTag53M

Plasmid

#26297

Depositor

George Cross

Type

Empty backbone

Use

Trypanosome in-situ tagging vector

Tags

triple cMyc

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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ZM2

Plasmid

#32784

Depositor

George Cross

Insert

T7rnap

Use

Cre/Lox; Floxed targeting vector for trypanosome

Available Since

Dec. 13, 2011

Availability

Academic Institutions and Nonprofits only
pENTR4-SB1 (w789-3)

Plasmid

#26956

Depositor

Eric Campeau

Type

Empty backbone

Use

Entry vector

Tags

SB1

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR4-CLIPf (w877-2)

Plasmid

#29650

Depositor

Eric Campeau

Type

Empty backbone

Use

Entry vector

Tags

CLIPf

Available Since

July 6, 2011

Availability

Academic Institutions and Nonprofits only

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