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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,392 results for: CIT

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Showing: 4361 - 4380 of 4392 results

  15. Venus-Puma-4E-pEGFP-C1

    Plasmid
    #166746
    Purpose
    Express Venus fused to the N-terminus of the Bcl-2 family protein, Puma with mutation in BH3 domain to disrupt binding anti-apoptotic proteins
    Depositor
    David Andrews
    Article
    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi
    Insert
    Puma-4E (BBC3 Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166746-plasmid-map-sequence-id-329007

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  19. pαH-S-GSAS-B.1.351.v4

    Plasmid
    #173788
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 4
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.351 variant version 4) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    173788-plasmid-map-sequence-id-352110

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  20. pGG442

    Plasmid
    #165616
    Purpose
    Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
    Use
    CRISPR
    Tags
    3x HA, SV40 NLS, Zif268, and c-Myc-like NLS
    Expression
    Yeast
    Mutation
    Corrected homology relative to wild type SpCas9 (…
    Promoter
    TEF1
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165616-plasmid-map-sequence-id-330958

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Showing: 4361 - 4380 of 4392 results