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pXP711

Plasmid

#46053

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

PGK1

Available Since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pNX3c2-HA3

Plasmid

#78392

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA
natCX

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pEMB44 / Zebrafish_LRRK2_1_338

Plasmid

#40556

Depositor

Michael J Fox Foundation MJFF

Insert

lrrk2 (lrrk2 Zebrafish)

Use

Baculovirus

Tags

6xHis

Mutation

WT

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pYTK049

Plasmid

#65156

Purpose

Encodes I-SceI recognition site as a Type 234 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

I-SceI recognition site

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pET22b-EC12-129/138

Plasmid

#47926

Purpose

Expresses E-cadherin EC12 with C9A, K129C/D138C mutations in E.Coli.

Depositor

Tanja Kortemme

Article

Ritterson et al J Am Chem Soc. 2013 Aug 7.

Insert

E-Cadherin EC12

Tags

6xHis and TEV Cleavage

Expression

Bacterial

Mutation

Mutations C9A, K129C, D138C

Promoter

T7

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pNR6

Plasmid

#21677

Depositor

James Haber

Article

Rudin et al Mol Cell Biol. 1988 Sep . 8(9):3918-28.

Insert

Ura3 (URA3 Budding Yeast)

Use

Integration sequence

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pAM75

Plasmid

#224249

Purpose

pET28a MWG-ubiquitin-G77-C78

Depositor

Andreas Martin

Article

Xie et al Methods Mol Biol. 2023;2591:79-100. doi:

Insert

MWG-ubiquitin-G77-C78

Expression

Bacterial

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAW8_tdTomato

Plasmid

#110239

Purpose

Cre/Lox C-terminal tagging construct encoding red fluorescent protein tdTomato

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_GST

Plasmid

#110237

Purpose

Cre/Lox C-terminal tagging construct encoding glutathione S-transferase

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_6XHis

Plasmid

#110236

Purpose

Cre/Lox C-terminal tagging construct encoding six histidine (His) residues

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_mCherry

Plasmid

#110238

Purpose

Cre/Lox C-terminal tagging construct encoding red fluorescent protein mCherry

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_13Myc

Plasmid

#110232

Purpose

Cre/Lox C-terminal tagging construct encoding 13 copies of the human c-myc (Myc) epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8_yEGFP

Plasmid

#110229

Purpose

Cre/Lox C-terminal tagging construct encoding codon-optimized green fluorescent protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_1XFLAG

Plasmid

#110234

Purpose

Cre/Lox C-terminal tagging construct encoding single copy of the FLAG epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_TAP

Plasmid

#110240

Purpose

Cre/Lox C-terminal tagging construct encoding the Twin Affinity Purification protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_3HA

Plasmid

#110233

Purpose

Cre/Lox C-terminal tagging construct encoding 3 copies of the influenza virus hemagglutunin (HA) epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_yECitrine

Plasmid

#110231

Purpose

Cre/Lox C-terminal tagging construct encoding codon-optimized yellow fluorescent protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pNX3a-HA3

Plasmid

#78359

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA
kanMX6

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pBN27

Plasmid

#35120

Depositor

John McCusker

Article

Hartzog et al Yeast. 2005 Jul 30;22(10):789-98.

Insert

fcy1delta1

Use

Yeast replicating

Mutation

amplicon has a complete deletion of FCY1 ORF, fla…

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pLEX307-TMPRSS2-puro

Plasmid

#158457

Purpose

Constitutive lentiviral expression of human TMPRSS2.

Depositor

Alejandro Chavez, Sho Iketani

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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