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pECNUS_P5

Plasmid

#194347

Purpose

sgRNA position entry clone 5

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Expression

Plant

Promoter

Ath U6 promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P6

Plasmid

#194348

Purpose

sgRNA position entry clone 6

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Expression

Plant

Promoter

Ath U6 promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P7

Plasmid

#194349

Purpose

sgRNA position entry clone 7

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Expression

Plant

Promoter

Ath U6 promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDHJS4 AS+

Plasmid

#78242

Purpose

One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).

Depositor

Tao-shih Hsieh

Article

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only
pDHJS4 BS-

Plasmid

#78245

Purpose

One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).

Depositor

Tao-shih Hsieh

Article

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only
pDHJS4 BS+

Plasmid

#78244

Purpose

One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).

Depositor

Tao-shih Hsieh

Article

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only
pDHJS4 AS-

Plasmid

#78243

Purpose

One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).

Depositor

Tao-shih Hsieh

Article

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Available Since

June 6, 2016

Availability

Academic Institutions and Nonprofits only
pTH644-CENBEVY

Plasmid

#29695

Depositor

Tobias von der Haar

Article

Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

Insert

bidirectional promoter cassette from pBEVY-U

Expression

Yeast

Available Since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only

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IDP205 (T7->Rci; 5bc)

Plasmid

#68834

Purpose

Expresses Rci gene (driven by the inducible T7 promoter) and 5 fragment barcode cassette in bacterial cells

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2014;42(16):e127. doi

Inserts

Barcode cassette 5 fragments
Rci recombinase

Promoter

T7

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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Pentaprobe PP5

Plasmid

#83330

Purpose

Pentaprobe for assesing the RNA binding potential of a protein

Depositor

Joel Mackay

Article

Bendak et al Nucleic Acids Res. 2012 Aug;40(14):e105.

Insert

PP5

Use

Rna transcription

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMXLC_pFa

Plasmid

#114219

Purpose

POC12355, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pTH645-RLuc

Plasmid

#29694

Depositor

Tobias von der Haar

Article

Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

Insert

Renilla Luciferase

Expression

Yeast

Available Since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pMXLK_dFq

Plasmid

#114237

Purpose

POC12377, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type d-q

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pJH25_pBad_AraC_Para-sfGFP.0TAG

Plasmid

#163720

Purpose

Arabinose-inducible expression of green fluorescent protein encoding zero amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

araC gfp

Expression

Bacterial

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMXLK_pFa

Plasmid

#114229

Purpose

POC12367, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ221

Plasmid

#132952

Purpose

crRNA entry vector of Prrk1-Not1-HHR with FnCas12a in Schizosaccharomyces pombe

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

FnCas12a

Use

CRISPR and Synthetic Biology

Promoter

adh1

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pYZ675

Plasmid

#132954

Purpose

crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a Schizosaccharomyces pombe

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

dFnCas12a

Use

CRISPR and Synthetic Biology

Tags

nucleoplasmin NLS

Promoter

adh1

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pYZ694

Plasmid

#132956

Purpose

crRNA entry vector of Prrk1-Not1-HHR with dFnCas12a-Clr4 in Schizosaccharomyces pombe

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

dFnCas12a

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS and nucleoplasmin NLS

Promoter

adh1

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBY2887

Plasmid

#23221

Depositor

Ralf Baumeister

Article

Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

Insert

SnAvi

Expression

Bacterial, Insect, and Mamm…

Available Since

Feb. 25, 2010

Availability

Academic Institutions and Nonprofits only

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pBY2807

Plasmid

#23222

Depositor

Ralf Baumeister

Article

Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

Insert

Gateway-SnAvi

Expression

Bacterial, Insect, and Mamm…

Available Since

Feb. 25, 2010

Availability

Academic Institutions and Nonprofits only
pEPYC1CB0199

Plasmid

#154655

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MAS Firefly luciferase expression cassette [MAS::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAptamerCassette-EGFP

Plasmid

#140288

Purpose

Backbone for making protein-responsive mRNA switches

Depositor

Hirohide Saito

Article

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJK504

Plasmid

#155381

Purpose

dCas12a crRNAs

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Insert

dCas12a oscillator crRNAs

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMXLP_pFa

Plasmid

#114239

Purpose

POC12379, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b-H74A-PvuRts1I

Plasmid

#86894

Purpose

express H74A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Tags

His

Expression

Bacterial

Mutation

W205A

Promoter

T7

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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Pentaprobe PP1

Plasmid

#83326

Purpose

Pentaprobe for assesing the RNA binding potential of a protein

Depositor

Joel Mackay

Article

Bendak et al Nucleic Acids Res. 2012 Aug;40(14):e105.

Insert

PP1

Use

Rna transcription

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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Pentaprobe PP12

Plasmid

#83337

Purpose

Pentaprobe for assesing the RNA binding potential of a protein

Depositor

Joel Mackay

Article

Bendak et al Nucleic Acids Res. 2012 Aug;40(14):e105.

Insert

PP12

Use

Rna transcription

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Pentaprobe PP11

Plasmid

#83336

Purpose

Pentaprobe for assesing the RNA binding potential of a protein

Depositor

Joel Mackay

Article

Bendak et al Nucleic Acids Res. 2012 Aug;40(14):e105.

Insert

PP11

Use

Rna transcription

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Pentaprobe PP10

Plasmid

#83335

Purpose

Pentaprobe for assesing the RNA binding potential of a protein

Depositor

Joel Mackay

Article

Bendak et al Nucleic Acids Res. 2012 Aug;40(14):e105.

Insert

PP10

Use

Rna transcription

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Pentaprobe PP9

Plasmid

#83334

Purpose

Pentaprobe for assesing the RNA binding potential of a protein

Depositor

Joel Mackay

Article

Bendak et al Nucleic Acids Res. 2012 Aug;40(14):e105.

Insert

PP9

Use

Rna transcription

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Pentaprobe PP8

Plasmid

#83333

Purpose

Pentaprobe for assesing the RNA binding potential of a protein

Depositor

Joel Mackay

Article

Bendak et al Nucleic Acids Res. 2012 Aug;40(14):e105.

Insert

PP8

Use

Rna transcription

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Pentaprobe PP7

Plasmid

#83332

Purpose

Pentaprobe for assesing the RNA binding potential of a protein

Depositor

Joel Mackay

Article

Bendak et al Nucleic Acids Res. 2012 Aug;40(14):e105.

Insert

PP7

Use

Rna transcription

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Pentaprobe PP6

Plasmid

#83331

Purpose

Pentaprobe for assesing the RNA binding potential of a protein

Depositor

Joel Mackay

Article

Bendak et al Nucleic Acids Res. 2012 Aug;40(14):e105.

Insert

PP6

Use

Rna transcription

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Pentaprobe PP4

Plasmid

#83329

Purpose

Pentaprobe for assesing the RNA binding potential of a protein

Depositor

Joel Mackay

Article

Bendak et al Nucleic Acids Res. 2012 Aug;40(14):e105.

Insert

PP4

Use

Rna transcription

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Pentaprobe PP3

Plasmid

#83328

Purpose

Pentaprobe for assesing the RNA binding potential of a protein

Depositor

Joel Mackay

Article

Bendak et al Nucleic Acids Res. 2012 Aug;40(14):e105.

Insert

PP3

Use

Rna transcription

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Pentaprobe PP2

Plasmid

#83327

Purpose

Pentaprobe for assesing the RNA binding potential of a protein

Depositor

Joel Mackay

Article

Bendak et al Nucleic Acids Res. 2012 Aug;40(14):e105.

Insert

PP2

Use

Rna transcription

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS413 Prp5 WT

Plasmid

#87599

Purpose

Encodes Prp5 WT for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 )

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pQuantA-puro-kana

Plasmid

#45586

Depositor

Andrzej Dziembowski

Article

Orlowska et al Nucleic Acids Res. 2013 Sep;41(17):e167.

Type

Empty backbone

Use

Cre/Lox; Dt40 cell line targeting

Tags

Quant-A tag

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pQuantA-bsr-kana

Plasmid

#45587

Depositor

Andrzej Dziembowski

Article

Orlowska et al Nucleic Acids Res. 2013 Sep;41(17):e167.

Type

Empty backbone

Use

Cre/Lox; Dt40 cell line targeting

Tags

Quant-A tag

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
ISA179: Tagatose pathway

Plasmid

#241968

Purpose

Phagemid for the tagatose pathway. Contains p15A, CmR, pac site, AraC, C1, and pBAD.

Depositor

Nathan Crook

Article

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

Tagatose pathway

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1553

Plasmid

#238470

Purpose

pMOBC360_VL: Vector Left (VL) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1554

Plasmid

#238471

Purpose

pMOBC360_VR: Vector Right (VR) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1358

Plasmid

#236103

Purpose

Assembled plasmid containing four inserts (from POC1343, POC1344, POC1345 and POC1346) assembled in POC1355

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1525

Plasmid

#226496

Purpose

pMOBC360_VM: Vector Middle (VM) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1551

Plasmid

#226498

Purpose

pMOBK360_StandVKL: Standard Vector (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1552

Plasmid

#226499

Purpose

pMOBC360_StandVC: Standard Vector (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1520

Plasmid

#221581

Purpose

pMOBK360_VR: Vector Right (VR) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1355

Plasmid

#221572

Purpose

Assembly vector designed to be methylated by the M.Osp807II BsaI-associated switch methylase and for the assembly of inserts 1, 2, 3 and 4 from POC1343, POC1344, POC1345 and POC1346; respectively

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1430

Plasmid

#221577

Purpose

Assembly vector designed to be methylated by the M.Osp807II BsaI-associated switch methylase and for the assembly of inserts 1, 2, 3 and 4 from POC1426, POC1427, POC1428 and POC1429; respectively

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1518

Plasmid

#221579

Purpose

pMOBK360_VL: Vector Left (VL) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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