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Plasmid#14504DepositorInsertIQGAP1 3'UTR mutant (IQGAP1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationGTGCCTT was changed to GTGCGCC and GTGCCT was cha…PromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLPC FLAG hRap1 delta CT
Plasmid#13252DepositorInsertRepressor Activator Protein 1 delta CT (TERF2IP Human)
UseRetroviralTagsFLAGExpressionMutationcontains hRap1 aa2-270 + 331-399 and the point mu…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTagBFP2-C1-AKAP1N31-FKBP-PI-PLCN187
Plasmid#139309PurposeN-terminal half of split PI-PLC targeted to mitochondriaDepositorInsertMus musculus Akap1(1-31) M16L: PTRSANS[GA]4ILSRM:TagBFP2: FKBP1A(3-108): [GGSA]4GG:L. monocytogenes PI-PLC(1-187)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/HisMaxB-YAP1-T110A
Plasmid#18996DepositorInsertYes-kinase associated protein T110A mutant (YAP1 Human)
UseTagsHisExpressionMammalianMutationThreonine 110 changed to AlaninePromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLPC FLAG hRap1 delta Br
Plasmid#13255DepositorInsertRepressor Activator Protein 1 delta Br (TERF2IP Human)
UseRetroviralTagsFLAGExpressionMutationcontains hRap1 aa2-44 + 96-399 and the point muta…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLPC myc-mRAP1 I312R
Plasmid#73137PurposeExpression of myc-tagged mRAP1 with single point mutatoin I312RDepositorInsertRap1 (Terf2ip Mouse)
UseTagsmycExpressionMammalianMutationisoleucine changed to an arginine at amino acid 3…PromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E360 Hs.YAP1-nostop
Plasmid#70644PurposeGateway ORF clone of human YAP1 [NM_001130145.2] without stop codon (for C-terminal fusions)DepositorInsertYAP1 (YAP1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Flag Yap1 Y357E
Plasmid#18883DepositorInsertYap1 Y357E (YAP1 Human)
UseTagsFlagExpressionMammalianMutationY357EPromoterAvailable sinceAug. 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_Y357F-PolyA
Plasmid#112287PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) Y357F mutant _ corresponding to tyrosine 407 in this isoformDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationTyrosine 357 to Phenyalanine corresponding to tyr…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/HisMaxB-YAP1-S343A
Plasmid#18992DepositorInsertYes-kinase associated protein S343A mutant (YAP1 Human)
UseTagsHisExpressionMammalianMutationSerine 343 changed to AlaninePromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/HisMaxB-YAP1-S358A
Plasmid#18994DepositorInsertYes-kinase associated protein S358A mutant (YAP1 Human)
UseTagsHisExpressionMammalianMutationSerine 358 changed to AlaninePromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/HisMaxB-YAP1-S103A
Plasmid#18980DepositorInsertYes-kinase associated protein S103A mutant (YAP1 Human)
UseTagsHisExpressionMammalianMutationSerine 103 changed to AlaninePromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAG415-ADH1p-HA-rap1-K9R
Plasmid#84531PurposeThis plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
UseTagsHAExpressionYeastMutationK(43,61,69,117,240,246,527,582,651)RPromoterADH1Available sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/HisMaxB-YAP1-S369A
Plasmid#18995DepositorInsertYes-kinase associated protein S369A mutant (YAP1 Human)
UseTagsHisExpressionMammalianMutationSerine 369 changed to AlaninePromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/HisMaxB-YAP1-S236A
Plasmid#18991DepositorInsertYes-kinase associated protein S236A mutant (YAP1 Human)
UseTagsHisExpressionMammalianMutationSerine 236 changed to AlaninePromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pML460_MAP1LC3B-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227901PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertMAP1LC3B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
LEGO-pDHS-AP1x6-GM55
Plasmid#217416PurposeLuciferase expression vector with 6 AP-1 sites and the minimal GM-CSF promoter, and a chromatin priming element. Alias: LEGO-GM55-AP-1x6-pDHS(IL-3)DepositorInsertLuciferase, GFP
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoter6 AP-1 sites, Human -55 to +28 minimal CSF2 promo…Available sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LEGO-pDHS-AP1x6-DUSP5
Plasmid#217418PurposeLuciferase expression vector with 6 AP-1 sites and the full length DUSP5 promoter, and a chromatin priming element. Alias: LEGO-DUSP5p-AP-1x6DepositorInsertLuciferase, GFP
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterHuman DUSP5 promoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-sCAG-eGFP-ACAP1
Plasmid#203800PurposeAAV vector plasmid expressing human ACAP1 fused to eGFP under a short variant of the CAG (sCAG) promoterDepositorInsertACAP1 (ACAP1 Human)
UseAAVTagseGFPExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGG-GST-TEV-NAP1 (WT)
Plasmid#198034PurposeUsed for the expression and purification of NAP1 in a mammalian expression system (SMC Internal No. 2064)DepositorInsertNAP1 (AZI2 Human)
UseTagsGST-TEVExpressionMammalianMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only