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Plasmid#123101PurposeExpresses 3xFLAG-GABARAPL1 G116A in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseTags3xFLAGExpressionMammalianMutationGlycine 116 to AlaninePromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAPL2 G116A
Plasmid#123087PurposeGateway entry clone encoding human GABARAPL2 G116ADepositorInsertGamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationGlycine 116 to AlaninePromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAP no-stop
Plasmid#123209PurposeGateway entry clone encoding human GABARAP lacking stop codon, suitable for C-terminal taggingDepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseGateway entry vector / entry cloneTagsExpressionMutationStop codon removedPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAPL1 G116A
Plasmid#123084PurposeGateway entry clone encoding human GABARAPL1 G116ADepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationGlycine 116 to AlaninePromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-TEVcs-ATG101
Plasmid#171414PurposeFor the purifcation of human ULK1 complexDepositorInsertATG101 (ATG101 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-TEVcs-GFP-ATG101
Plasmid#171415PurposeFor the purifcation of GFP-ULK1 complexDepositorInsertATG101 (ATG101 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAP G116
Plasmid#123082PurposeGateway entry clone encoding human GABARAP G116DepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseGateway entry vector / entry cloneTagsExpressionMutationDeleted amino acid 117. Stop codon after G116PromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-GFP-ATG14
Plasmid#171426PurposeFor the purifcation of GFP- PI3KC3 complexDepositorInsertATG14 (ATG14 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBMN iRFP670-NDP52(C443K)
Plasmid#119695PurposeFor mammalian expression of iRFP-tagged UBD mutant NDP52DepositorInsertNDP52 (CALCOCO2 Human)
UseRetroviralTagsiRFP670ExpressionMammalianMutationC443KPromoterAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAPL1 G116
Plasmid#123085PurposeGateway entry clone encoding human GABARAPL1 G116DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseGateway entry vector / entry cloneTagsExpressionMutationDeleted amino acid 117. Stop codon after G116PromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMN mCherry-NBR1(F562A/Y731A/ΔUBD)
Plasmid#119692PurposeFor mammalian expression of LIR double mutant NBR1 without its UBDDepositorInsertNBR1 (NBR1 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationF562A/Y731A & UBD deletion (1-906 ), See depo…PromoterAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMN iRFP670-OPTN(F178A/D474N)
Plasmid#119694PurposeFor mammalian expression of iRFP-tagged LIR/UBD double mutant OPTNDepositorInsertOPTN (OPTN Human)
UseRetroviralTagsiRFP670ExpressionMammalianMutationF178A/D474NPromoterAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
Human ubiquitination-related proteins CRISPR KO library
Pooled Library#174592PurposeCRISPR Cas9 KO library targets 660 ubiquitination-related proteins, including all major E1, E2, E3 and deubiquitinating enzymes, as well as other ubiquitination-related proteins.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmStrawberry-Atg4BC74A
Plasmid#21076DepositorInsertAtg4B(C74A) (Atg4b Mouse)
UseTagsmStrawberryExpressionMammalianMutation220-222 TGC(Cys) is changed to GCA (Ala)PromoterAvailable sinceJune 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
Rtn3LΔ6LIR-mNeonGreen
Plasmid#169036PurposeExpress human Rtn3L with 6x LC3-interacting regions mutated in mammalian cellsDepositorInsertRtn3L (RTN3 Human)
UseTagsmNeonGreenExpressionMammalianMutationChanged following LC3-interacting regions of Rtn3…PromoterCMVAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hGABARAP
Plasmid#87871PurposeExpress a EGFP-human GABARAP in mammalian cellsDepositorInsertGABARAP (GABARAP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV GFP-GABARAPL1 WT
Plasmid#123117PurposeExpresses EGFP-GABARAPL1 wild-type in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV 3xFLAG-GABARAPL1-GFP
Plasmid#123112PurposeExpresses 3xFLAG-GABARAPL1-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL1DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseTags3xFLAG and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV 3xFLAG-GABARAP-GFP
Plasmid#123111PurposeExpresses 3xFLAG-GABARAP-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPDepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseTags3xFLAG and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-GABARAPL2 G116A
Plasmid#123104PurposeExpresses 3xFLAG-GABARAPL2 G116A in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
UseTags3xFLAGExpressionMammalianMutationGlycine 116 to AlaninePromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only