We narrowed to 26,966 results for: STI

1. 221-pCAGGS-HA-AID-CTCFcDNA-AID-eGFP-2A-blasticidin

   Plasmid

   #195101

   Purpose

   Vector to introduce human CTCF cDNA tagged with eGFP and two AIDs in the cells. Blasticidin selection (2A-fusion). Auxin-inducible degron system.

   Depositor

   Job Dekker

   Article

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Insert

   CTCF (CTCF Human)
   

   Use

   Tags

   AID, HA, and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGS

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2

   Plasmid

   #182350

   Purpose

   Ubiquitous expression cloning vector for mosTI(unc-119). Transgene inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

   Plasmid

   #187945

   Purpose

   dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   SpdCas9-tagRFPt-P2A-tagBFP
   

   Use

   Synthetic Biology; Piggybac

   Tags

   NLS, NLS + HA-tag, P2A, tagBFP, and tagRFPt

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Padh1-OsTIR1(F74A)-NLS-TADH1-Sp.arg3+/pUC19*

   Plasmid

   #169353

   Purpose

   Expression of OsTIR1(F74A) in S. pombe from adh1 promoter, integrated at arg3-D4 locus

   Depositor

   Tony Carr

   Insert

   OsTIR1(F74A)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   F74A

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLX208-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

   Plasmid

   #125231

   Purpose

   For generating SPARK2 NanoLuc-βarrestin2-TEVp lentivirus

   Depositor

   Alice Ting

   Article

   Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

   Insert

   NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSEM386 - mosTI unc-119 - NNN - gfp - tbb-2

   Plasmid

   #182345

   Purpose

   GFP fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A

   Plasmid

   #182049

   Purpose

   expression of Sox2 mutants

   Depositor

   Hyonchol Jang

   Article

   Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

   Insert

   Sox2 (Sox2 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S248A/T258A

   Promoter

   CAG

   Available since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV-SFFV-myc-STIM1-WPRE-UbC-Emerald

   Plasmid

   #225939

   Purpose

   Lentiviral vector plasmid expressing human stromal interaction molecule 1 (STIM1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Evan Reid, Bart Nieuwenhuis, Richard Eva

   Inserts

   STIM1 (STIM1 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV-SFFV-myc-STIM2-WPRE-UbC-Emerald

   Plasmid

   #225940

   Purpose

   Lentiviral vector plasmid expressing human stromal interaction molecule 2 (STIM2) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Evan Reid, Bart Nieuwenhuis, Richard Eva

   Inserts

   STIM2 (STIM2 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSAH46 - MosTI [unc-119(partial) | MCS | gfp]

    Plasmid

    #200366

    Purpose

    Cloning vector for mosTI(unc-119, gfp). MCS or Golden Gate.

    Depositor

    Christian Froekjaer-Jensen

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSAH45 - MosTI [unc-119(partial) | MCS | wrmScarlet]

    Plasmid

    #200365

    Purpose

    Cloning vector for mosTI(unc-119, wrmScarlet). MCS or Golden Gate.

    Depositor

    Christian Froekjaer-Jensen

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSEM323 - array co-injection fragment mosTI Pmlc-2_GFP

    Plasmid

    #159836

    Purpose

    Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    array co-injection fragment mosTI Pmlc-2_GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

    Plasmid

    #89873

    Purpose

    expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    bArrestin2-TevC-P2A-TdTomato
    

    Use

    AAV

    Tags

    TdTomato

    Expression

    Mammalian

    Mutation

    R173H in TdTomato (Please see depositor comments …

    Promoter

    Human synapsin

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSEM414 - mosTI unc-119 Peft-3 - gfp(nls) - tbb-2

    Plasmid

    #182357

    Purpose

    Positive control for mosTI(unc-119). unc-119 rescue and ubiquitous nuclear GFP expression after mosTI insertion.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Peft-3

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSEM396 - mosTI unc-119 - NNN - operon - gfp - tbb-2

    Plasmid

    #182353

    Purpose

    GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PCDNA3-muOR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

    Plasmid

    #113611

    Purpose

    PPI reporter

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

    Insert

    mu opioid receptor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)

    Plasmid

    #38262

    Depositor

    Robert Lefkowitz

    Article

    Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

    Insert

    β-arrestin2 (Arrb2 Rat)
    

    Use

    Tags

    Flag and PIP5K Iα core kinase domain (residues 18…

    Expression

    Mammalian

    Mutation

    Arg 233, Arg237, and Lys251 converted to Glutamin…

    Promoter

    CMV

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP

    Plasmid

    #196988

    Purpose

    Derived from pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP, custom sgRNA cloning vector

    Depositor

    Elizabeth Kirby

    Article

    Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

    Insert

    Nestin-dCas9-KRAB-T2a-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PCDNA3-b2AR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

    Plasmid

    #113610

    Purpose

    PPI reporter

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

    Insert

    beta 2 adrenergic receptor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PCDNA3-PAR1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

    Plasmid

    #113608

    Purpose

    PPI reporter

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

    Insert

    PAR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge