We narrowed to 16,639 results for: SUB

1. pCDNA3_nmPKA-Cα-mPAGFP

   Plasmid

   #114131

   Purpose

   Mammalian expression of the G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with G2A mutation (Prkaca Mouse)
   

   Expression

   Mammalian

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3_PKA-Cα(S11D)-mPAGFP

   Plasmid

   #114135

   Purpose

   Mammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11D mutation (Prkaca Mouse)
   

   Expression

   Mammalian

   Available Since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3_PKA-Cα(S11A)-mPAGFP

   Plasmid

   #114134

   Purpose

   Mammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11A mutation (Prkaca Mouse)
   

   Expression

   Mammalian

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3_PKA-CαN-mPAGFP

   Plasmid

   #114114

   Purpose

   Mammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP (Prkaca Mouse)
   

   Expression

   Mammalian

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-nAChr-Alpha6

   Plasmid

   #48812

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3_nmPKA-CαN-mPAGFP

   Plasmid

   #114130

   Purpose

   Mammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP, with G2A mutation (Prkaca Mouse)
   

   Expression

   Mammalian

   Available Since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-nAChr-Alpha6-mCherry

   Plasmid

   #50480

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Tags

   mCherry fusion in M3-M4 loop (after residue A405)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pJK100

   Plasmid

   #73264

   Purpose

   Produces Bacillus subtilis phosphoribosylformylglycinamidine synthase small subunit (BsPurS)

   Depositor

   T. J. Kappock

   Insert

   phosphoribosylformylglycinamidine synthase subunit PurS
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-nAChr-Alpha6-GFP

   Plasmid

   #50486

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Tags

   GFP fusion in M3-M4 loop (after residue A405)

   Expression

   Mammalian

   Mutation

   Gly-Ala-Gly flexible linker flanking the GFP open…

   Promoter

   CMV

   Available Since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1-nAChr-Alpha6-YFP

    Plasmid

    #50483

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Tags

    YFP fusion in M3-M4 loop (after residue A405)

    Expression

    Mammalian

    Mutation

    Gly-Ala-Gly flexible linker flanking the YFP open…

    Promoter

    CMV

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1-nAChr-Alpha6-CFP

    Plasmid

    #50482

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Tags

    CFP fusion in M3-M4 loop (after residue A405)

    Expression

    Mammalian

    Mutation

    Gly-Ala-Gly flexible linker flanking the CFP open…

    Promoter

    CMV

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pJK611

    Plasmid

    #72532

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit (AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Expression

    Bacterial

    Promoter

    T7 and n/a

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. p11d-tRPA-32Asp8

    Plasmid

    #102617

    Purpose

    Expression of human RPA with aspartic acid substituted phosphorylation domain

    Depositor

    Marc Wold

    Article

    Binz et al J Biol Chem. 2003 Sep 12;278(37):35584-9

    Inserts

    RPA1 (RPA1 Human)
    RPA3 (RPA3 )
    RPA2 (RPA2 )
    

    Expression

    Bacterial

    Mutation

    with aspartic acid substituted phosphorylation do…

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX307-mFzd8

    Plasmid

    #102870

    Purpose

    Expresses mouse Fzd8 in mammalian cells

    Depositor

    Henry Ho

    Article

    Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

    Insert

    Frizzled 8 (Fzd8 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    T to C substitution at nucleotide 1134, C to T su…

    Promoter

    EF1A

    Available Since

    Jan. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK610

    Plasmid

    #72531

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit wutg C-terminal His6 tag (AaPurLH6)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7 and n/a

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK543

    Plasmid

    #72516

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); large subunit (AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Expression

    Bacterial

    Mutation

    contains additional Ala2

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJK623

    Plasmid

    #72540

    Purpose

    Produces Acetobacter aceti 1023 purSyQL associated hypothetical protein; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit (AaOrfY, AaPurQ, AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV4 p65

    Plasmid

    #21966

    Depositor

    Warner Greene

    Article

    Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

    Insert

    NFkB p65 subunit (RELA Human)
    

    Expression

    Mammalian

    Mutation

    This construct was the product of 3 subcloning pr…

    Available Since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK624

    Plasmid

    #72539

    Purpose

    Produces Acetobacter aceti 1023 OrfY; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit with C-terminal His6 tag (AaOrfY, AaPurQ, AaPurLH6)

    Depositor

    T. J. Kappock

    Inserts

    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJet1.2 ermB cassette

    Plasmid

    #117121

    Purpose

    Erythromycin resistance cassette

    Depositor

    Stephan Gruber

    Article

    Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

    Insert

    Erythromycin resistance cassette for Bacillus subtilis selection
    

    Use

    Golden gate donor vector for gene targeting in ba…

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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