-
Plasmid#122564PurposeVector for endogenously tagging Giardia genes with a triple HA tag (Puromycin selection)DepositorTypeEmpty backboneUseTagshemagglutinin tagExpressionMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only
-
pKS_3HA_BSR
Plasmid#122567PurposeVector for endogenously tagging Giardia genes with a triple HA tag (Blasticidin selection)DepositorTypeEmpty backboneUseTags3x hemagglutinin tagExpressionMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
KSP_K406
Plasmid#129771PurposeExpresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assaysDepositorInsertKSP (kif11.L Frog)
UseTagsHis6ExpressionBacterialMutationTruncation at 367, fused to kinesin-1 starting Al…PromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pResQ shFEN3 3XF-FEN1 DeltaC
Plasmid#17754DepositorInsertFlap Endonuclease I (FEN1 Human)
UseLentiviral and RNAiTagsTriple FlagExpressionMammalianMutationDeletion of C-terminal 20 amino acids from FEN1PromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKS_3MYC_PAC
Plasmid#122566PurposeVector for endogenously tagging Giardia genes with a triple Myc tag (Puromycin selection)DepositorTypeEmpty backboneUseTags3x c-Myc tagExpressionMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKS_3HA_NEO
Plasmid#122565PurposeVector for endogenously tagging Giardia genes with a triple HA tag (G418 selection)DepositorTypeEmpty backboneUseTagshemagglutinin tagExpressionMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3(D3A,V266E)
Plasmid#90091PurposeExpresses caspase D3A and V266E mutant in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationD175A D28A D9A V266EPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQC V5 HuR.wt IRES Puro
Plasmid#110386Purposeγ-Retroviral transfer vector for expressing HuR (wild type), IRES-driven Puromycin selection.DepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3
Plasmid#90087PurposeExpresses wild type caspase 3 in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationWild typePromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
-
pQC V5 mKO2 HuR.wt IRES Puro
Plasmid#110387Purposeγ-Retroviral transfer vector for expressing HuR (wild type), V5 and mKO2 tags, IRES-driven Puromycin selection.DepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsV5 and mKO2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3(C163S)
Plasmid#90088PurposeExpresses caspase 3 with C163S to prevent autocatalysis in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationC163SPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-FoxG1
Plasmid#35547DepositorInsertFoxG1 (Foxg1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pELPS-eDHFR-YFP-T2A-Renilla
Plasmid#193253PurposeTriple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferaseDepositorInsertThe eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
UseLentiviralTagsRenilla luciferase (rLuc) and yellow fluorescent …ExpressionMutationNonePromoterEF1aAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-CeeIF4G_315-491opt_AB
Plasmid#148349PurposeBacterial Expression of CeeIF4G_315-491optDepositorInsertCeeIF4G_315-491opt (ifg-1 Nematode)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ptet-RFP-shR-rtTA
Plasmid#35625PurposeTet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).DepositorTypeEmpty backboneUseAAV and RNAi; Tet inducibleTagsExpressionMammalianMutationPromoterPtetAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIREShygro HA-mVenus-mAID
Plasmid#117714PurposeExpression of HA-tagged mVenus fused to mAIDDepositorInsertmVenus-mAID
UseTagstriple HAExpressionMammalianMutationcodon optimized mAID for murine expressionPromoterCMVAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
csd5_41-192/pET28b(+)
Plasmid#83296Purposecsd5, construct: 41-192, pET28b(+) N-tagDepositorInserthp1250
UseTagsHis tagExpressionBacterialMutationdeleted amino acid 1-40PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd4_22-438/pET28b(+)
Plasmid#83295Purposecsd4, construct: 22-438, pET28b(+) N-tagDepositorInserthp1075
UseTagsHis tagExpressionBacterialMutationdeleted amino acid 1-21PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only