Addgene

We narrowed to 1,214 results for: appa

Showing: 421 - 440 of 1214 results

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CtkA_D179Q_pET21a

Plasmid

#27431

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Nov 22. (

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Use

Tags

His

Expression

Bacterial

Mutation

D179Q

Promoter

Available since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_D155Q/D179Q_pET21a

Plasmid

#27432

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Nov 22. (

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Use

Tags

His

Expression

Bacterial

Mutation

D155Q/D179Q

Promoter

Available since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
pRCA595 - pBA904 Puro-T2A-mCherry

Plasmid

#238171

Purpose

Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

Insert

Non-targeting sgRNA

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA594 - pBA904 Puro-T2A-BFP

Plasmid

#238170

Purpose

Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

Insert

Non-targeting sgRNA

Use

CRISPR and Lentiviral

Tags

mTagBFP2

Expression

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGB-Co-NF-kB

Plasmid

#180206

Purpose

Expression GAL4-fusion of Co-NF-kB in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

Co-NF-kB

Use

Tags

GAL4 (1-147) fusion

Expression

Yeast

Mutation

aa 2-1224

Promoter

AdhI

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-GalT-Sirius

Plasmid

#174469

Purpose

Express Sirius with golgi apparatus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with golgi apparatus-targeting sequence

Use

Tags

β1,4-galactose transferase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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miR-223-RFP pCDNA

Plasmid

#97142

Purpose

expression of miR-223 and TagRFP

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

miR-223

Use

Tags

TagRFP

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSI-check2-TAB2-3'UTR

Plasmid

#97157

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

TAB2 3'UTR (TAB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-SAC1C389S-HLx8

Plasmid

#108140

Purpose

Inactive SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)

Promoter

CMV

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pEBB-3XMyc-TRAF2 S327A

Plasmid

#44108

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S327A (TRAF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Serine 327 to Alanine

Promoter

EF1α

Available since

May 2, 2013

Availability

Academic Institutions and Nonprofits only
lentiCRISPRi(v2)-Blast

Plasmid

#170068

Purpose

Expresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistance

Depositor

Neville Sanjana

Article

Morris et al Science. 2023 May 19;380(6646):eadh7699.

Insert

KRAB-dCas9-MeCP2

Use

CRISPR and Lentiviral

Tags

2A and 2xHA tag

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Cas12aUltra_5XNLS

Plasmid

#218775

Purpose

His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS

Depositor

Ross Wilson

Article

Sahu et al bioRxiv 2024.07.14.603391

Insert

5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a

Use

CRISPR

Tags

6XHis (C terminal), HA, Nucleoplasmin, SV40, and …

Expression

Bacterial

Mutation

Nucleotides mutations were made to encode for arg…

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-VAPB

Plasmid

#108126

Purpose

ER marker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:VAPB (VAPB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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EGFR WT

Plasmid

#11011

Purpose

Retroviral construct for expressing human EGFR in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pEBB Flag GCN5

Plasmid

#74784

Purpose

express of GCN5 in mammalian cells

Depositor

Ezra Burstein

Article

Mao et al Genes Dev. 2009 Apr 1;23(7):849-61. doi:

Insert

GCN5 (KAT2A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF-1alpha promoter

Available since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-Ubiquitin

Plasmid

#187910

Purpose

Mammalian cell expression of EGFP-tagged Ubiquitin

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

UBB (UBB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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TFAM-SNAPf

Plasmid

#187909

Purpose

Mammalian cell expression of SNAPf-labeled TFAM

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

TFAM (TFAM Human)

Use

Tags

SNAPf

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP-mCherry

Plasmid

#108122

Purpose

Recruitable fluorophore

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

FKBP1A(isoform a, 3-108):mCherry (FKBP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-FLAG-Ubc13-WT

Plasmid

#43938

Depositor

Bryant Darnay

Article

Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

Insert

ubiquitin-conjugating enzyme E2N (UBE2N Human)

Use

Tags

2x FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 myc card9

Plasmid

#16253

Depositor

William Kaelin

Article

Yang et al Mol Cell. 2007 Oct 12. 28(1):15-27.

Insert

card9 (CARD9 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

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