We narrowed to 10,572 results for: cycs

1. pACYCDuet1-ammA(∆Trp56)

   Plasmid

   #134683

   Purpose

   Expresses His6-AmmA(∆Trp56) in E. Coli

   Depositor

   Wilfred van der Donk

   Article

   Ting et al Science. 2019 Jul 19;365(6450):280-284.

   Insert

   ammA(delW56)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   deletion of W56

   Promoter

   T7

   Available since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. UASp-deltaN cycB

   Plasmid

   #112967

   Purpose

   P element vector for transposon insertion of nondegradable Cyclin B into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Lee et al Genetics. 2001 Aug;158(4):1545-56.

   Insert

   cycB (CycB Fly)
   

   Use

   P insertion vector

   Tags

   Expression

   Mutation

   N-term deletion of first 46 amino acids, includes…

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. LHP809 - Ub-K63A-CYC1

   Plasmid

   #32166

   Depositor

   Linda Hicke

   Insert

   synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K63A

   Promoter

   CUP1

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

4. Rc/CMV cyclin D2 E7K HA

   Plasmid

   #8955

   Depositor

   Philip Hinds

   Article

   Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

   Insert

   cyclin D2 (CCND2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E7K: point mutation in LxCxE motif, LxCxk

   Promoter

   Available since

   Oct. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pNatB (pACYCduet-naa20-naa25)

   Plasmid

   #53613

   Purpose

   Allows expression of the fission yeast NatB complex - chloramphenicol marker

   Depositor

   Dan Mulvihill

   Article

   Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

   Inserts

   naa20 (naa20 Fission Yeast)
   naa25 (naa25 Fission Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFA6a-kanMX-tetO-Pcyc1

   Plasmid

   #41023

   Depositor

   Michael Nick Boddy

   Article

   Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

   Insert

   tetO7-Pcyc1
   

   Use

   Promoter replacement

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. PBAD-his6-prkA-pACYC184

   Plasmid

   #41041

   Depositor

   Ichiro Matsumura

   Article

   Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

   Insert

   prkA
   

   Use

   Tags

   6xHis and T7 epitope tag

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pACYC-FLAG-dN6-His

   Plasmid

   #22143

   Depositor

   Robert Sauer

   Article

   Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

   Insert

   Flag-clpXdeltaNLinkedHexamer-His6
   

   Use

   Tags

   Flag, His6, and L20 Linkers

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNatA (pACYCduet-naa10-naa15)

   Plasmid

   #72928

   Purpose

   Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

   Inserts

   naa10 (naa10 Synthetic, Fission Yeast)
   naa15 (naa15 Fission Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optomised for E.coli expression

   Promoter

   T7

   Available since

   Feb. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX-2TK cyclin T1 (P#468)

    Plasmid

    #14607

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

    Insert

    cyclin T1 (CCNT1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

11. p414-TEF1p-Cas9-CYC1t

    Plasmid

    #43802

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pF1-Hs Cdk1/cyclin B1/Cks1

    Plasmid

    #177329

    Purpose

    Co-expression of the human active Cdk1/cyclin B1/Cks1 kinase complex

    Depositor

    Andreas Boland

    Article

    Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

    Inserts

    Cyclin-dependent kinase 1 (CDK1 Human)
    G2/mitotic-specific cyclin-B1 (CCNB1 Human)
    Cyclin-dependent kinases regulatory subunit 1 (CKS1B Human)
    

    Use

    Tags

    2 x Strep-tag and 8 x His

    Expression

    Insect

    Mutation

    Promoter

    p10 and polh

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p415-GalL-Cas9-CYC1t

    Plasmid

    #43804

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GalL

    Available since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. D. vulgaris sp2 CRISPR/pACYCDuet-1

    Plasmid

    #81186

    Purpose

    Expresses CRISPR array containing 3 copies of Desulfovibrio vulgaris spacer 2 and flanking repeats in E. coli

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Synthetic CRISPR array (3 copies of D. vulgaris spacer #2 flanked by repeats)
    

    Use

    CRISPR

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pACYC-T7-Nla-repeat-BbsI

    Plasmid

    #180215

    Purpose

    For cloning Neisseria lactamica Type I-C crRNA for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLIB-HIS-TEV-Cyclin-B

    Plasmid

    #177012

    Purpose

    Generate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEV

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    Cyclin B1 (CCNB1 Human)
    

    Use

    Tags

    Polyhistidine-TEV

    Expression

    Insect

    Mutation

    codon-optimised for insect cell expression

    Promoter

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

    Plasmid

    #83946

    Purpose

    Cas9-carrying vector with kanMX marker

    Depositor

    Irina Borodina

    Article

    Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX 6P1 Cyclin A (555)

    Plasmid

    #61844

    Purpose

    bacterial expression of GST cyclin A

    Depositor

    Jonathon Pines

    Insert

    cyclin A (CCNA2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    L2V

    Promoter

    tac

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pLVX-TetOne-FFSS-Cyclin D1

    Plasmid

    #215146

    Purpose

    Expresses FFSS-Cyclin D1 in mammalian cells from an inducible lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    FFSS (FLAG-FLAG-STREP-STREP)

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CycA-Venus-Flag (1305)

    Plasmid

    #39853

    Depositor

    Jonathon Pines

    Article

    Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

    Insert

    Cyclin A (CCNA2 Human)
    

    Use

    Tags

    Flag and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only