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Showing: 421 - 440 of 80703 results

  1. pcDNA3.1 Flag YFP hNKCC1 extracellular-cysteineless (NT864)

    Plasmid
    #49069
    Purpose
    Expresses human NKCC1 mutant lacking extracellular cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C563S,C568S,C577S,C582S in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49069-plasmid-map-sequence-id-72196

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  2. pET-15b(+) Mortierella verticillate (delta prenylation motif)

    Plasmid
    #170788
    Purpose
    Expresses pET-15b(+) Mortierella verticillate (delta prenylation motif)
    Depositor
    Kalle Gehring
    Insert
    Mortierella verticillate PRL
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lacI
    Available Since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    170788-plasmid-map-sequence-id-337769

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  6. pcDNA3.1 Flag YFP hNKCC1 intracellular-cysteineless (NT855)

    Plasmid
    #49080
    Purpose
    Expresses human NKCC1 mutant lacking cysteine residues within intracellular loops and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C791S, C820S, C1052S in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Nov. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    49080-plasmid-map-sequence-id-72210

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  7. Cultivarium POSSUM Toolkit

    Plasmid Kit
    #1000000234
    Purpose
    The POSSUM Toolkit is a Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model microbes.
    Depositor
    Nili Ostrov
    Available Since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only

  8. Cancer Pathways ORFs Kit

    Plasmid Kit
    #1000000072
    Purpose
    A set of vectors containing cDNAs for studying oncogenic signaling pathways. cDNAs are either wild-type, constitutively active mutants, or dominant negative mutants.
    Depositor
    David Sabatini, Kris Wood
    Available Since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. dRMCE Plasmids

    Plasmid Kit
    #1000000015
    Purpose
    Takes advantage of wild-type loxP and FRT sites present in these conditional alleles in mouse embryonic stem cells to introduce tags, reporters, and mutant coding regions into an endogenous loci
    Depositor
    Rolf Zeller
    Available Since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  10. biGBac Kit

    Plasmid Kit
    #1000000088
    Purpose
    Generation of baculoviral transfer vectors coding up to 25 protein complex subunits in two Gibson assembly steps. Multisubunit protein complexes can be generated in baculovirus-infected insect cells.
    Depositor
    Jan-Michael Peters
    Available Since
    Oct. 25, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. Phosphoinositide Biosensors Kit

    Plasmid Kit
    #1000000261
    Purpose
    Use the phosphoinositide biosensors kit to detect the whole family of phosphoinositides in fixed cells and tissues.
    Depositor
    David Murray
    Available Since
    July 9, 2025
    Availability
    Academic Institutions and Nonprofits only

  12. Hamdoun lab FP fusion plasmids

    Plasmid Kit
    #1000000033
    Purpose
    Screen fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed, e.g. zebrafish, sea urchin, frog (Xenopus), or worm (C. elegans)
    Depositor
    Amro Hamdoun
    Available Since
    July 24, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. Integrated Genomics Plasmids

    Plasmid Kit
    #1000000004
    Purpose
    Plasmids used in Integrated Genomics: A Discovery-Based Laboratory Course; please note that the C. elegans yeast two-hybrid cDNA library is unavailable
    Depositor
    Guy Caldwell
    Available Since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  14. pCri System

    Plasmid Kit
    #1000000058
    Purpose
    pCri System: plasmids for cytoplasmic and periplasmic/extracellular heterologous protein expression in Escherichia coli, Bacillus subtilis, and Pichia pastoris.
    Depositor
    F Xavier Gomis-Ruth
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. MoClo Yeast Secretion and Display (YSD) Toolkit

    Plasmid Kit
    #1000000240
    Purpose
    The YSD Toolkit is a collection of 34 plasmids that can be used in conjunction with the MoClo Yeast Toolkit (YTK) to optimize secretion and cell surface display of proteins of interest in yeast.
    Depositor
    Paul Young
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only

  16. CRISPRainbow Kit for Multiplex Labeling

    Plasmid Kit
    #1000000079
    Purpose
    CRISPR/Cas9 to tag up to 7 different genomic locations. Unique labeling system allows scientists to track DNA movement in live cells and study the structure of the genome in real time.
    Depositor
    Thoru Pederson
    Available Since
    June 8, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. AID* Kit

    Plasmid Kit
    #1000000120
    Purpose
    Expanded tool kit for the auxin-inducible degron (AID) system in yeast ( S. cerevisiae). Includes tags for easy detection by antibodies/microscopy and a set of selection markers.
    Depositor
    Helle Ulrich
    Available Since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  18. Yeast GPCR-sensor Toolkit

    Plasmid Kit
    #1000000157
    Purpose
    The Yeast GPCR-sensor Toolkit includes 42 plasmids for constructing highly-tunable GPCR-based biosensors in yeast.
    Depositor
    Tom Ellis
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only

  19. Ubiquiton In Vitro Kit

    Plasmid Kit
    #1000000242
    Purpose
    The Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.
    Depositor
    Helle Ulrich
    Available Since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only

  20. Komagataeibacter Tool Kit (KTK)

    Plasmid Kit
    #1000000216
    Purpose
    The Komagataeibacter Tool Kit (KTK) is a standardized modular cloning system based on Golden Gate DNA assembly.
    Depositor
    Tom Ellis
    Available Since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 421 - 440 of 80703 results