Addgene

We narrowed to 15,854 results for: por

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SMN2E7-Fluc-Nluc-reporter

Plasmid

#221005

Purpose

Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.

Depositor

Mikko Taipale, Benjamin Blencowe

Article

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Insert

Fluc-SMN2E7-STOP-Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Sport6-CD9-pHuji

Plasmid

#130906

Purpose

Expression of CD9-pHuji for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD9-pHuji (CD9 Human, Synthetic)

Use

Tags

pHuji

Expression

Mammalian

Mutation

pHuji inserted between Asn50 and Asn51 in extrace…

Promoter

CMV

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pSV Sport PPAR gamma 2

Plasmid

#8862

Depositor

Bruce Spiegelman

Article

Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

Insert

PPAR gamma (Pparg Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2006

Availability

Academic Institutions and Nonprofits only

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miRFP718nano-based inflammation reporter

Plasmid

#195753

Purpose

Reporter of inflamation

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

NF-kB-RE-miRFP718nano

Use

Tags

miRFP718nano

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSV Sport PPAR gamma 1

Plasmid

#8886

Depositor

Bruce Spiegelman

Article

Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

Insert

PPAR gamma (Pparg Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-K9 histone methylation reporter

Plasmid

#22866

Depositor

Alice Ting

Article

Lin et al J Am Chem Soc. 2004 May 19. 126(19):5982

Insert

K9 HMR

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pLV-REPORT(PGK)-12xHRE

Plasmid

#172334

Purpose

hypoxic responsive reporter

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

monomeric nuclear Tomato
d2eGFP
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Mutation

Promoter

PGK

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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V5-BS2-SENP2 (Nuclear Pore)

Plasmid

#220345

Purpose

Mammalian expression vector BS2 in nuclear pore

Depositor

Bryan Dickinson

Article

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Use

Tags

SENP2 (nuclear pore localized protein) and V5 tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJC5-4-insulator-BREreporter-insulator

Plasmid

#171499

Purpose

injections to make Bmp reporter mouse transgenic line

Depositor

James Briscoe

Article

Ivanovitch et al PLoS Biol. 2021 May 17;19(5):e3001200. d

Insert

Insulator BRE-MLP:H2B-Turqoise

Use

Tags

Expression

Mammalian

Mutation

Promoter

BRE-MLP

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-FL

Plasmid

#34597

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
eGFP Y93H CC editing reporter

Plasmid

#198885

Purpose

Express eGFP fluorescence after Y93H correction and mCherry as transfection control

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

enhanced green fluorescent protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Y93H, Gly92(GGT-to-GGC)

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSport6/FLAG-Wwtr1(Taz)

Plasmid

#27318

Depositor

Jonathan Epstein

Insert

Taz (Wwtr1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
sgRNA1_Tet-inducible Luciferase reporter

Plasmid

#64161

Purpose

Photoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1 for Tet-inducible Luciferase reporter

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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betaTrCP mVenus mutant reporter

Plasmid

#192459

Purpose

Fluorescent reporter for beta-TrCP with a non-degradable mutation. Used as a control.

Depositor

Steven Cappell

Article

Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

Insert

CdC25B

Use

Lentiviral

Tags

SV40 NLS and mVenus

Expression

Mammalian

Mutation

aa198-338 of CDC25B. betaTrCP degron sequence (aa…

Promoter

EF1alpha

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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eGFP Y93H AC editing reporter

Plasmid

#198883

Purpose

Express eGFP fluorescence after Y93H correction and mCherry as transfection control

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

enhanced green fluorescent protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Y93H, Gly92(GGT-to-GGA)

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eGFP Y93H GC editing reporter

Plasmid

#198884

Purpose

Express eGFP fluorescence after Y93H correction and mCherry as transfection control

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

enhanced green fluorescent protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Y93H, Gly92(GGT-to-GGG)

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Lepr peak reporter-eGFP

Plasmid

#196584

Purpose

Cis-regulatory region of mouse Lepr gene driven a eGFP reporter with hGFP driven rtTA3

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

eGFP

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-SPORT6-RNF114-RINGmut

Plasmid

#69922

Purpose

Expresses mutant myc-RNF114 in mammalian cells

Depositor

Francesca Capon

Article

Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

Insert

RNF114 (RNF114 Human)

Use

Tags

Expression

Mammalian

Mutation

C29A, C32A

Promoter

CMV

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
T7 promoter + rpsL reporter plasmid

Plasmid

#156455

Purpose

A mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Inserts

rpsL
TcR

Use

Tags

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

tac and tet

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-SPORT6-RNF114-UIMmut

Plasmid

#69923

Purpose

Expresses mutant myc-RNF114 in mammalian cells

Depositor

Francesca Capon

Article

Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

Insert

RNF114 (RNF114 Human)

Use

Tags

Expression

Mammalian

Mutation

S224P

Promoter

CMV

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only