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pTol1-U6abc_amhc(myh6)_cmlc2-nmKate

Plasmid

#238375

Purpose

Drives expression of 3 different gRNAs targeting amhc (myh6), and expression of nclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting amhc

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-cmlc2-nGFP-nCas9

Plasmid

#238308

Purpose

Expression of nuclear GFP and nuclear Cas9 specifically in cardiomyocytes driven by the cmlc2(myl7) promoter.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

Cas9 (Csn1) endonuclease from Streptococcus pyogenes

Use

Tol2-based plasmid

Tags

nGFP-P2A

Promoter

cmlc2 (myl7)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRE3G-ZIM3-Cas9-P2A-GFP

Plasmid

#239605

Purpose

Doxycycline inducible CRISPRgenee construct

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

ZIM3 KRAB domain (ZIM3 )

Use

CRISPR and Lentiviral

Promoter

TRE3G

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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EF1as-ZIM3-Cas9-P2A-GFP

Plasmid

#239603

Purpose

Constitutive active CRISPRgenee construct

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

ZIM3 KRAB domain (ZIM3 )

Use

CRISPR and Lentiviral

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pTE3: pFuGW-SSX1p-GAL4BD-1X3CLpro consensus CS-VP16AD-SV40NLS

Plasmid

#240456

Purpose

A synthetic transcription factor (synTF) that contains concensus 3CLpro cleavage site (CS)

Depositor

Lior Nissim

Article

Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

Inserts

SSX1p
GAL4BD-1X3CLpro consensus CS-VP16AD-SV40NLS

Use

Lentiviral

Promoter

SSX1 and SSX1p

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pSE163: pFuGW-SSX1p-GAL4BD-1X3CLpro synthetic general CS -VP16AD-SV40NLS

Plasmid

#240457

Purpose

A synthetic transcription factor (synTF) that contains a synthetic general 3CLpro cleavage site (CS)

Depositor

Lior Nissim

Article

Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

Inserts

SSX1p
GAL4BD-1X3CLpro synthetic general CS -VP16AD-SV40NLS

Use

Lentiviral

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5: pFuGW-SSX1p- GAL4BD-4X3CLpro synthetic general CS-VP16AD-SV40NLS

Plasmid

#240460

Purpose

a synthetic transcription factor (synTF) that contains 4 tandem synthetic general 3CLpro cleavage site (CS).

Depositor

Lior Nissim

Article

Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

Inserts

SSX1p
GAL4BD-4X3CLpro synthetic general CS-VP16AD-SV40NLS

Use

Lentiviral

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPB758

Plasmid

#210033

Purpose

Contains inducible Cas7-P2A-Cas5-T2A-Cas8, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas7-P2A-Cas5-T2A-Cas8
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1α and TRE3G

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSCrhaB2_ApyOAHID

Plasmid

#226245

Purpose

Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Inserts

His6_ApyA
ApyD
ApyO
ApyH
ApyI

Tags

Hexa-Histidine

Expression

Bacterial

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-ZipT-2A-IvfChr (citrine, KV2.1)

Plasmid

#221619

Purpose

A recombinant AAV2 plasmid encoding the ZipACR I151T mutant with soma targeting with KV2.1 motif and membrane expression enhanced vfChrimson-Citrine with soma targeting KV2.1 motif.

Depositor

John Lin

Article

Mermet-Joret et al eLife 2023 12:RP90327

Insert

ZipACR (I151V)KV2.1-2A-IvfChr-citrine-Kv2.1

Use

AAV

Mutation

ZipACR (I151T) with soma targeting, vfChrimson w…

Promoter

Human Synapsin

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-ZipT-2A-IvfChr (mCherry, KV2.1)

Plasmid

#221621

Purpose

A recombinant AAV2 plasmid encoding the ZipACR I151T mutant with soma targeting with KV2.1 motif and membrane expression enhanced vfChrimson-mCHerry with soma targeting KV2.1 motif.

Depositor

John Lin

Article

Mermet-Joret et al eLife 2023 12:RP90327

Insert

ZipACR (I151V)KV2.1-2A-IvfChr-mCherry-Kv2.1

Use

AAV

Mutation

ZipACR (I151T) with soma targeting, vfChrimson w…

Promoter

Human Synapsin

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-ZipV-2A-IvfChr (citrine, KV2.1)

Plasmid

#221618

Purpose

A recombinant AAV2 plasmid encoding the ZipACR I151V mutant with soma targeting with KV2.1 motif and membrane expression enhanced vfChrimson-Citrine with soma targeting KV2.1 motif.

Depositor

John Lin

Article

Mermet-Joret et al eLife 2023 12:RP90327

Insert

ZipACR (I151V)KV2.1-2A-IvfChr-citrine-Kv2.1

Use

AAV

Mutation

ZipACR (I151V) with soma targeting, vfChrimson w…

Promoter

Human Synapsin

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR

Plasmid

#218916

Purpose

Lentiviral construct expressing msfGFP and nuclear mCherry proteins.

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

EF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR

Use

Lentiviral

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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CCM2_I432D_eGFP

Plasmid

#208879

Purpose

Express eGFP-CCM2 with the I432D mutation in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM2 (CCM2 Human)

Tags

eGFP

Expression

Mammalian

Mutation

mutation I432D

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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CCM2_W421AD422A_eGFP

Plasmid

#208880

Purpose

Express eGFP-CCM2 with the W421A and D422A mutations in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM2 (CCM2 Human)

Tags

eGFP

Expression

Mammalian

Mutation

mutations W421A and D422A

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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CCM2_I428S_eGFP

Plasmid

#208878

Purpose

Express eGFP-CCM2 with the I428S mutation in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM2 (CCM2 Human)

Tags

eGFP

Expression

Mammalian

Mutation

mutation I428S

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA

Plasmid

#174895

Purpose

amber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

102TAG 150TAA in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST40-CES2C-ΔC

Plasmid

#200555

Purpose

Mammalian expression of secreted mouse CES2C

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2C delta C-terminal (Ces2c Mouse)

Tags

6xHis, Flag, and V5

Expression

Mammalian

Promoter

CMV promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(103)-GFP

Plasmid

#197890

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS(553)-GFP

Plasmid

#197888

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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