We narrowed to 23,297 results for: promoter

1. qTAG-AAVS1-Ef1a-Zeo

   Plasmid

   #227268

   Purpose

   Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-hBcan

   Plasmid

   #18966

   Depositor

   Mariano Viapiano

   Article

   Hu et al J Biol Chem. 2008 Sep 5;283(36):24848-59

   Insert

   Brevican (BCAN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-E1.1-RFP

   Plasmid

   #22928

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

   Insert

   E1.1 binding site from Scardigli et al., 2003 with minimal CMV
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. FL-BCAN-lentiviral-GFP

   Plasmid

   #29704

   Depositor

   Mariano Viapiano

   Article

   Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

   Insert

   Brevican (BCAN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pscALPS gag-gfp/vpx

   Plasmid

   #115808

   Purpose

   SFFV promoter expresses gag-gfp fusion with CypA promoter driving expression of SIVMAC251 vpx

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   gag-gfp
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pscALPS gag-gfp/deltavpx

   Plasmid

   #115807

   Purpose

   SFFV promoter expresses gag-gfp fusion with no ORF after CypA promoter

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   gag-gfp
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-nucGFP11-3xControlgRNA

   Plasmid

   #224568

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   GFP11-H2B
   

   Use

   CRISPR

   Tags

   Histone H2B (nuclear localization)

   Expression

   Mutation

   Codon optimized

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe-puro-Smad4-Flag

   Plasmid

   #37041

   Depositor

   Sam Thiagalingam

   Article

   Papageorgis et al Cancer Res. 2011 Feb 1;71(3):998-1008. E

   Insert

   Smad4 (SMAD4 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   5'LTR

   Available since

   March 1, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pGL3 GATA Luc

   Plasmid

   #85695

   Purpose

   Luciferase reporter containing three repeats of the GATA consensus site

   Depositor

   Giannino Del Sal, Licio Collavin

   Article

   Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

   Insert

   3xGATA and minimal MT promoter
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   3xGATA and minimal MT promoter

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1 HA-rnSOX9

    Plasmid

    #62972

    Purpose

    Expresses HA-tagged rat SOX9

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    SOX9 (Sox9 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

11. GFP-PKCA D463N

    Plasmid

    #195203

    Purpose

    Mammalian expression vector for GFP tagged PKCA. Kinase-inactive mutant

    Depositor

    David Bryant, Peter Parker

    Article

    Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

    Insert

    PRKCA (PRKCA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D463N

    Promoter

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. His10-PS-SNAPf (XSB522)

    Plasmid

    #78512

    Purpose

    Construct for expressing His10 - SNAPf in E. Coli

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    SNAPf
    

    Use

    Tags

    His10 followed by a prescission protease claevage…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-SpCas9_sgRNA-site1 (RTW443)

    Plasmid

    #160136

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. hOC-GFPtpz

    Plasmid

    #110207

    Purpose

    Expresses GFP in mature bone cells.

    Depositor

    David Rowe

    Article

    Bilic-Curcic et al Genesis. 2005 Oct;43(2):87-98. doi: 10.1

    Insert

    eGFPtopaz
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Human osteocalcin (BGLAP)

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACT2-CAS9c

    Plasmid

    #51055

    Purpose

    Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    Cas9c
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    yeast ADH1 promoter

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRE2-Bla(HA–RILP)

    Plasmid

    #102425

    Purpose

    HA tag fused to the N-terminus of RILP for the expression in mammalian cells.

    Depositor

    Steven Weinman

    Article

    Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    Rab interacting lysosomal protein (RILP Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-responsive

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBP-J23100

    Plasmid

    #72963

    Purpose

    Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    J23100 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC-hU6-crScaffold_SV40-BFP

    Plasmid

    #224860

    Purpose

    crRNA scaffold for RfxCas13d expressed from hU6 promoter and reporter BFP protein expressed from SV40 promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZmUbi-5'UTR

    Plasmid

    #154057

    Purpose

    Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct)

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    Promoter and 5' UTR Ubiquitin (Zea mays)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-nDsRedFL-intron

    Plasmid

    #177804

    Purpose

    An intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    intron
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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