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2XMyc-LRRK2-3XKD

Plasmid

#25366

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

"Kinase Dead" Lysine 1906 mutated to A…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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Flag-betaPIXa

Plasmid

#15234

Depositor

Rick Horwitz

Article

Mayhew et al J Proteome Res. 2006 Sep . 5(9):2417-23.

Insert

beta-PIXa (Arhgef7 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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pT-CA-GFP-IRES-mLumin

Plasmid

#32749

Depositor

Jeanne Hardy

Article

Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

Inserts

CA-GFP
mLumin

Expression

Mammalian

Mutation

S158A and S65T

Promoter

CMV

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-TYR

Plasmid

#32781

Depositor

Ruth Halaban

Article

Halaban et al Proc Natl Acad Sci U S A. 2000 May 23;97

Insert

Tyrosinase (TYR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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H2B-Orange

Plasmid

#20969

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Histone cluster 1, H2bb (H2BC3 Human)

Tags

Orange

Expression

Mammalian

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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FKBP-myc

Plasmid

#20211

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FKBP (FKBP1A Human)

Tags

myc

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
p3869 HA-E6-AP C833A

Plasmid

#8649

Depositor

Peter Howley

Article

Talis et al J Biol Chem 1998 Mar 13;273(11):6439-45.

Insert

E6-AP (UBE3A Human)

Tags

HA

Expression

Mammalian

Mutation

C833A; active site Cys mutated to encode Ala

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only
pNeuroD-ires-GFP

Plasmid

#61403

Purpose

Mammalian expression vector under the neuron-specific promoter NeuroD. Co-expression of GFP with IRES sequence

Depositor

Franck Polleux

Article

Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

Type

Empty backbone

Expression

Mammalian

Promoter

NeuroD

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pHR' CMV GFP

Plasmid

#14858

Depositor

Inder Verma

Article

Miyoshi et al Proc Natl Acad Sci U S A. 1997 Sep 16. 9

Insert

CMV-GFP

Use

Lentiviral

Expression

Mammalian

Available Since

May 11, 2007

Availability

Academic Institutions and Nonprofits only

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MO91-dCas9-AID(P182X)

Plasmid

#83260

Purpose

Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.

Depositor

Xing Chang

Article

Ma et al Nat Methods. 2016 Dec;13(12):1029-1035.

Insert

dCas9-AID P182X

Use

Retroviral

Tags

3xFLAG-NLS and FLAG

Expression

Mammalian

Mutation

D10A and H840A in Cas9; P182X in AID

Promoter

MSCV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only
LV-TRE-WT human MyoD-T2A-dsRedExpress2

Plasmid

#60628

Purpose

Expresses WT human MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

VP64 mouse MyoD (MYOD1 Human)

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-EGFP-WPRE-pA

Plasmid

#37084

Purpose

Cre-dependent expression of EGFP

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

EGFP

Use

AAV and Cre/Lox; Cre-on

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA-SET-FLAG-HA

Plasmid

#24998

Depositor

Judy Lieberman

Article

Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

Insert

SET (SET Human)

Tags

FLAG and HA

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pCS-memb-mCherry

Plasmid

#53750

Purpose

Contains mCherry fused to a 2x membrane localization sequence in the pCS2+ vector.

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

2x membrane linker

Tags

mCherry

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pTEC19

Plasmid

#30178

Depositor

Lalita Ramakrishnan

Article

Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

Crimson

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pAC-hRluc

Plasmid

#24348

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

hRluc

Expression

Insect

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

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m168

Plasmid

#34886

Depositor

Irvin Chen

Article

Morizono et al Nat Med. 2005 Mar;11(3):346-52. Epub 200

Insert

Sindbis virus envelope protein

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(C128S/D156A)-EYFP

Plasmid

#35503

Purpose

AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2(C128S/D156A)-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

C128S and D156A

Promoter

Ef1a

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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Myc-tagged human full length TCF4E pcDNA3

Plasmid

#32738

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

TCF4E (TCF7L2 Human)

Tags

Myc

Expression

Mammalian

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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Mfn2(K109A)-16xmyc

Plasmid

#26051

Depositor

David Chan

Article

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Mfn2 (Mfn2 Mouse)

Tags

16xMyc

Expression

Mammalian

Mutation

K109A GTPase mutant

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only