We narrowed to 4,788 results for: LUM

1. pcDNA5/FRT/TO_CalR_HaloTag9_KDEL

   Plasmid

   #175540

   Purpose

   Expression of HaloTag9 in the endoplasmid reticulum of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   CalR-HaloTag9-KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT-Bip-GFP:V5:KDEL

   Plasmid

   #69917

   Purpose

   An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

   Depositor

   Kenneth Irvine

   Article

   Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

   Insert

   GFP
   

   Use

   Tags

   Drosophila BiP signal sequence, KDEL ER targeting…

   Expression

   Insect

   Mutation

   Promoter

   pMT

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF.myc.ER-E2-Crimson

   Plasmid

   #38770

   Purpose

   In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

   Depositor

   Benjamin Glick

   Article

   Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

   Insert

   E2-Crimson
   

   Use

   Tags

   ER retention signal (KDEL), ER signal sequence (M…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. Str-KDEL_neomycin

   Plasmid

   #65306

   Purpose

   Luminal ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
   

   Use

   Tags

   KDEL motif and Signal Sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMpGWB402-er-mCherry

   Plasmid

   #186288

   Purpose

   Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

   Insert

   er-mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmTurquoise2-ER

   Plasmid

   #36204

   Purpose

   In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   ER-mTurquoise2
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDusk

   Plasmid

   #43795

   Purpose

   Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

   Depositor

   Andreas Moeglich

   Article

   Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO_CalR-SNAP-tag2-KDEL

   Plasmid

   #226517

   Purpose

   Mamalian cell expression of SNAP-tag2 in endoplasmic reticulum

   Depositor

   Kai Johnsson

   Article

   Kühn et al bioRxiv 2024.08.28.610127

   Insert

   CalR-SNAP-tag2-KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LRTi

   Plasmid

   #214072

   Purpose

   Luminescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Linker sequence present between firefly luciferas…

   Promoter

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LRTX_ctrl

    Plasmid

    #214073

    Purpose

    Luminescent reporter system, NMD negative control. No intron in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Remnant of linker sequence present at the end of …

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEFIRES-P-mTagBFP-KDEL

    Plasmid

    #87163

    Purpose

    Fluorescent marker of endoplasmic reticulum (ER)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    mTagBFP-KDEL
    

    Use

    Tags

    Bip signal peptide (Homo sapiens) and mTagBFP-KDE…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1 alpha

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. LRT_RTctrl

    Plasmid

    #214074

    Purpose

    Luminescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LRT

    Plasmid

    #214071

    Purpose

    Luminescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAPV-DsRed2-ER-5

    Plasmid

    #170570

    Purpose

    Expresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    APV-DsRed2-ER-5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. iRFP-FRB-ER

    Plasmid

    #139317

    Purpose

    endoplasmic reticulum-resident FRB for chemically induced dimerization

    Depositor

    Gerry Hammond

    Article

    Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    iRFP:MTOR(2021-2113):[GGSA]2ILNSRV:SACM1L(521-587)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMDC32-Kar2-CFP-HDEL

    Plasmid

    #96989

    Purpose

    Express endoplasmic reticulum (ER) marker in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

    Insert

    Kar2-CFP-HDEL
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO_CalR_HaloTag7_KDEL

    Plasmid

    #175530

    Purpose

    Expression of HaloTag7 in the endoplasmid reticulum of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CalR-HaloTag7-KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIgnaviCas9

    Plasmid

    #127595

    Purpose

    Expresses IgnaviCas9 in E. coli

    Depositor

    Stephen Quake

    Article

    Schmidt et al Proc Natl Acad Sci U S A. 2019 Oct 28. p

    Insert

    IgnaviCas9
    

    Use

    Tags

    6xHis Tag and Maltose binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LiRT

    Plasmid

    #214075

    Purpose

    Luminescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LiRT_RTctrl

    Plasmid

    #214078

    Purpose

    Luminescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge