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p7xEGFP-N1

Plasmid

#122167

Purpose

Expression of 7xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

7xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Cas9m3

Plasmid

#47315

Purpose

Cas9 D10A+D839A+H840A

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m3

Use

CRISPR

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pQE80_T5_P3-ehaA_LacI

Plasmid

#186315

Purpose

Plasmid for inducible E coli expression of the P3 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

P3-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_AP4-ehaA_LacI

Plasmid

#186316

Purpose

Plasmid for inducible E coli expression of the AP4 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

AP4-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEGF/HisB-cGK1

Plasmid

#106451

Purpose

expression of murine cyclic GMP-dependent kinase

Depositor

Georg Weber

Article

He et al Biochem Biophys Res Commun. 2004 Aug 13;

Insert

cyclic GMP-dependent kinase (Prkg1 Mouse)

Tags

6xHis

Expression

Mammalian

Mutation

WT

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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p9xEGFP-N1

Plasmid

#122169

Purpose

Expression of 9xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

9xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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p11xEGFP-N1

Plasmid

#122171

Purpose

Expression of 11xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

11xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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PM-TD!TB

Plasmid

#48656

Purpose

Bacterial TD crRNA expression: targets TD to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial TD crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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FISSEQ-hgRNA-Design2

Plasmid

#100572

Purpose

FISSEQ-hgRNA-Design2 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

FISSEQ-hgRNA-Design2

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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SK-YFP-TD-B

Plasmid

#48663

Purpose

Bacterial TD repression YFP reporter: protospacer B

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TD prototspacer B/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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PM-NM!TA

Plasmid

#48651

Purpose

Bacterial NM crRNA expression: targets NM to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial NM crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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PM-TD!TA

Plasmid

#48655

Purpose

Bacterial TD crRNA expression: targets TD to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial TD crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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PM-NM!TB

Plasmid

#48652

Purpose

Bacterial NM crRNA expression: targets NM to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial NM crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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PM-SP!TA

Plasmid

#48649

Purpose

Bacterial SP crRNA expression: targets SP to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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FISSEQ-hgRNA-Design1

Plasmid

#100571

Purpose

FISSEQ-hgRNA-Design1 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

FISSEQ-hgRNA-Design1

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 P279L Cas2

Plasmid

#80095

Purpose

Encodes Cas1 P279L and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

P279L

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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PTGR-PS2

Plasmid

#237442

Purpose

Expressing PS2 S-layer from C. glutamicum under its native promoter

Depositor

Han Remaut

Article

Sogues et al Proc. Natl. Acad. Sci. U.S.A. 122 (31) e

Insert

cpsB

Expression

Bacterial

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-SynCas.v10 mammalian expression

Plasmid

#208589

Purpose

Expression of SynCas.v10 for analysis of RNA knockdown

Depositor

Omar Akbari

Article

Brogan et al bioRxiv 2024.02.23.581838; doi: https://

Insert

SynCas.v10

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-SynCas.v14A mammalian expression

Plasmid

#208590

Purpose

Expression of SynCas.v14A for analysis of RNA knockdown

Depositor

Omar Akbari

Article

Brogan et al bioRxiv 2024.02.23.581838; doi: https://

Insert

SynCas.v14A

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-SynCas.v35 mammalian expression

Plasmid

#208591

Purpose

Expression of SynCas.v35 for analysis of RNA knockdown

Depositor

Omar Akbari

Article

Brogan et al bioRxiv 2024.02.23.581838; doi: https://

Insert

SynCas.v35

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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