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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,543 results for: LEA

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Showing: 4421 - 4440 of 15543 results

  3. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

    Plasmid
    #217808
    Purpose
    Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217808-plasmid-map-sequence-id-489173

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  4. pGBW-m4046865

    Plasmid
    #153930
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153930-plasmid-map-sequence-id-307315

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  5. pOCC119

    Plasmid
    #118884
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mEGFP-stop-HindIII cassette
    Tags
    6xHis, MBP, cleavable with 3C protease and mEGFP,…
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118884-plasmid-map-sequence-id-232361

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  10. NES-Grx1-roGFP2

    Plasmid
    #236551
    Purpose
    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the cytoplasm.
    Depositor
    Pablo Rivera-Fuentes
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NES-Grx1-roGFP2
    Tags
    nuclear export signal
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236551-plasmid-map-sequence-id-469946

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  11. AWP-029

    Plasmid
    #213966
    Purpose
    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for gRNA constitutive expression.
    Depositor
    Alexander Westermann
    Publication
    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121
    Insert
    Cas12a (cas12a Synthetic, Segatella bryantii)
    Tags
    3XFLAG
    Expression
    Bacterial
    Mutation
    D875A to inactivate target nuclease activity
    Promoter
    PcfxA, IPTG inducible
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213966-plasmid-map-sequence-id-429164

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  16. pcDNA3.1+ GEMIN6 myc HIS

    Plasmid
    #71659
    Purpose
    Mammalian expression of GEMIN6
    Depositor
    Gideon Dreyfuss
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GEMIN6 (GEMIN6 Human)
    Tags
    HIS and myc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. pX330-Flag-dSpCas9

    Plasmid
    #92113
    Purpose
    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead/inactive SpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    Cbh
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92113-plasmid-map-sequence-id-197502

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Showing: 4421 - 4440 of 15543 results