We narrowed to 7,257 results for: trac

1. pSECRETS-A

   Plasmid

   #196986

   Purpose

   For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).

   Depositor

   Eric Josephs

   Article

   Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pltetO1

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3918-37

   Plasmid

   #176495

   Purpose

   pTRE-EGFP-T2A-mCherry-polyA

   Depositor

   Albert La Spada

   Article

   Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

   Insert

   Tetracyline Responsive Element driving EGFP-T2A-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. MXS_rtTA3

   Plasmid

   #62428

   Purpose

   MXS_chaining vector with rtTA3

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   Reverse tetracycline transactivator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pASKt15C+SrtA

   Plasmid

   #64983

   Purpose

   Expresses the wild type of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

   Insert

   sortase A
   

   Use

   Tags

   His6 tag and S tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59

   Promoter

   tetracycline promoter/operator

   Available since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVH234-Tier1-PmPGK1-tTA

   Plasmid

   #169574

   Purpose

   Tier-1 vector encoding PmPGK1-driven tTA expression (PmPGK1-tTA-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled transactivator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDM1203

   Plasmid

   #108983

   Purpose

   extrachromosomal expression vector

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Type

   Empty backbone

   Use

   Dictyostelium discoideum expression vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 1025

   Plasmid

   #160235

   Purpose

   FlightN-TetR(OFF)-VP16-Sv40-attB

   Depositor

   Omar Akbari

   Article

   Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

   Inserts

   Tetracycline repressor
   Flightin promoter
   

   Use

   Tags

   VP16

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cherry-tTA-ER-C3

   Plasmid

   #134567

   Purpose

   Expresses a Cherry-tTA (tetracycline transcriptional activator)-ER (estrogen receptor hormone binding domain) fusion protein

   Depositor

   Susan Janicki

   Article

   Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

   Insert

   tTA-ER
   

   Use

   Tags

   Cherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNG2

   Plasmid

   #30986

   Depositor

   Daniel Voytas, Adam Bogdanove

   Article

   Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

   Insert

   repeat NG2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. MXS_Tet

    Plasmid

    #62422

    Purpose

    MXS_chaining vector with Tet

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    Tetracycline inducible promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMars1CyeGFP

    Plasmid

    #75222

    Purpose

    Mammalian intracellular expression of fluorogen activating protein Mars1Cy-eGFP fusion

    Depositor

    Alan Waggoner

    Article

    Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

    Insert

    Mars1Cy-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. MXS_tTA2

    Plasmid

    #62429

    Purpose

    MXS_chaining vector with rTA2

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    Tetracycline transactivator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCSJ95

    Plasmid

    #104343

    Purpose

    Express yeast Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

    Depositor

    Alexander Varshavsky

    Article

    Chen et al Science. 2017 Jan 27;355(6323). pii: 355

    Insert

    FBP1
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVYD

    Plasmid

    #61302

    Purpose

    Vanillate-inducible gene expression plasmid for Sphingomonas melonis Fr1; destination vector for Gateway cloning, allowing C-terminal fusion of proteins to SYFP2

    Depositor

    Julia Vorholt

    Article

    Kaczmarczyk et al Sci Rep. 2014 Sep 29;4:6453. doi: 10.103

    Type

    Empty backbone

    Use

    Tags

    SYFP2

    Expression

    Bacterial

    Mutation

    Promoter

    PV10

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSJ125

    Plasmid

    #104344

    Purpose

    Express yeast MDH2-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

    Depositor

    Alexander Varshavsky

    Article

    Chen et al Science. 2017 Jan 27;355(6323). pii: 355

    Insert

    MDH2
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIE2-AD

    Plasmid

    #21603

    Depositor

    Takahiro Kusakabe

    Article

    Mon et al Anal Biochem. 2009 May 26. ():.

    Insert

    NF-kappa-B p65 subunit (Rela Mouse)
    

    Use

    Gateway destination vector

    Tags

    Flag, Gateway destination cassette, and NLS

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNG3

    Plasmid

    #30987

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    repeat NG3
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGPS21loxFRTNeo

    Plasmid

    #27180

    Depositor

    Yoh Wada

    Article

    Aoyama et al Nucleic Acids Res. 2005 . 33(5):e52.

    Insert

    lox/Frt/-PGK/EM7pm-G418/Kan-lox/Frt cassette flanked by Tn7L/R
    

    Use

    In vitro transposion

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA 3.1 CMV NAPA-N BGH

    Plasmid

    #92278

    Purpose

    FRET probe; extracellular synapse targeted mCherry

    Depositor

    Baljit Khakh

    Article

    Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

    Insert

    Neuron-astrocyte proximity assay - Neuronal
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNI2

    Plasmid

    #30997

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    repeat NI2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge