We narrowed to 8,876 results for: sgrna

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pgRNA-non-target

Plasmid

#129465

Purpose

Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Non-target/random gRNA

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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U6-Nme2_tracr

Plasmid

#192146

Purpose

Expresses Nme2-sgRNA cloned with BspQI

Depositor

Yongming Wang

Article

Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

Insert

Nme2 sgRNA scaffold

Use

AAV

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-sgLmnb2-HepmCherry

Plasmid

#192830

Purpose

Expresses sgRNA targeting Lmnb2 and mCherry from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 for sgRNA and hepatocyte-specific promoter (HS…

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCBC-DT1T2.2

Plasmid

#134752

Purpose

PCR template for sgRNA cloning

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

sgRNA-AtU6_29p

Use

CRISPR

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCBC-MT1T2.2

Plasmid

#134751

Purpose

PCR template for sgRNA cloning

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

sgRNA-TaU3p

Use

CRISPR

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTAG1

Plasmid

#176863

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_1

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK087

Plasmid

#171359

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

P-ANtRNA[Pro1]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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U6::sgBFP-U6::sgKrasG12D-pX333-red

Plasmid

#149448

Purpose

pX333 backbone expressing 2 sgRNA targeting BFP and KRAS G12D to edit mouse KRAS G12D allele.

Depositor

Jonathan Chernoff

Article

Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

Insert

sgRNA BFP and mouse KRAS G12D

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG2E

Plasmid

#176865

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_2E

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pC1.530

Plasmid

#205441

Purpose

Level 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

RepB gRNA

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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