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pAct-GST-H3(K27Q)

Plasmid

#182843

Purpose

a K27Q point mutation (AAG/CAG) of Drosophila histone H3 was generated from pAct-GST-H3. Expression in S2 cells.

Depositor

Peter Harte

Article

Tie et al Development. 2009 Sep;136(18):3131-41. d

Insert

GST-histone H3 fusion

Tags

GST

Expression

Insect

Promoter

Actin5C

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAct-GST-H3

Plasmid

#182842

Purpose

A PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.

Depositor

Peter Harte

Article

Tie et al Development. 2009 Sep;136(18):3131-41. d

Insert

GST-histone H3 fusion

Tags

GST

Expression

Insect

Promoter

Actin5C

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0080

Plasmid

#172786

Purpose

Level 0 module containing NT2_nVenus

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

3X FLAG CtmVENUS 210-238 RSIAT pICH41258 (SP NT2)

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0131

Plasmid

#172793

Purpose

Level 0 module containing CDS2ns_deltamPTAC5

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS mature PTAC5 with C-ter delection (41-252), Arabidopsis thaliana

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0010

Plasmid

#172797

Purpose

Level 1 module containing 35S-HSP21-GFP_P2 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-NT2_CTPSSU-mHSP21-GFP-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0020

Plasmid

#172798

Purpose

Level 1 module containing 35S-HSP21-nYFP_notag_P2 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mHSP21-nYFP-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0030

Plasmid

#172799

Purpose

Level 1 module containing 35S-HSP21-cYFP_notag_P3 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mHSP21-cYFP-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0040

Plasmid

#172800

Purpose

Level 1 module containing 35S-HSP21-nYFP_P2 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mHSP21-3XFLAGnYFP-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0060

Plasmid

#172802

Purpose

Level 1 module containing 35S-nYFP-HSP21_P2 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-3XFLAGnYFP-mHSP21-CT_STOP-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0070

Plasmid

#172803

Purpose

Level 1 module containing 35S-cYFP-HSP21_P3 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-cYFP3XHA-mHSP21-CT_STOP-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0080

Plasmid

#172804

Purpose

Level 1 module containing 35S-HSP21-nVENUS_P2 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mHSP21-nVENUS-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0090

Plasmid

#172805

Purpose

Level 1 module containing 35S-HSP21-cVENUS_P3 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mHSP21-3XFLAGcVENUS-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0100

Plasmid

#172806

Purpose

Level 1 module containing 35S-PTAC5-cYFP_P3 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mPTAC5-cYFP3XHA-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0110

Plasmid

#172807

Purpose

Level 1 module containing 35S-deltaPTAC5-cYFP_P3 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-deltamPTAC5-cYFP3XHA-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0020

Plasmid

#172776

Purpose

Level 0 module containing NT1_OEP7

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS of chloroplast outer envelope membrane protein OEP7

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pJH3971

Plasmid

#177736

Purpose

Prgef-1 GCaMP6::3xNLS::mNeptune

Depositor

Mei Zhen

Article

Susoy et al Cell. 2021 Sep 30;184(20):5122-5137.e17.

Insert

GCaMP

Tags

mNeptune

Expression

Worm

Promoter

Prgef-1

Available Since

Dec. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAB-NEO-PYL

Plasmid

#114393

Purpose

For PYL expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL

Tags

3xFLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMRBad-C-DiB-RM

Plasmid

#168478

Purpose

C-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168476: pET11a-N-DiB-RM to obtain DiB-RM‐split protein

Depositor

Jens Meiler

Article

Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

Insert

C-fragment of DiB-RM

Expression

Bacterial

Promoter

araBAD

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET11a-N-DiB-RM

Plasmid

#168476

Purpose

N-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168478: pMRBad-C-DiB-RM to obtain DiB-RM‐split protein

Depositor

Jens Meiler

Article

Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

Insert

N-fragment of DiB-RM

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc120

Plasmid

#161950

Purpose

Contains elements for building reporter plasmid for IBM. Built upon pSB4A3. ECF20 reporter plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB4A3-PECF20-B30-mScarlet-I-B15

Expression

Bacterial

Promoter

PECF20

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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