We narrowed to 13,654 results for: crispr cas9

1. pET28b-SpRY-His

   Plasmid

   #179320

   Purpose

   Plasmid for bacterial expression and purification of SpRY-Cas9

   Depositor

   Julián Cerón, Miguel Angel Moreno-Mateos

   Article

   Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

   Insert

   Human codon-optimized Cas9 variant SpRY
   

   Use

   CRISPR

   Tags

   6xHis and SV40-NLS

   Expression

   Bacterial

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28b-SpG-His

   Plasmid

   #179318

   Purpose

   Plasmid for bacterial expression and purification of SpG-Cas9

   Depositor

   Julián Cerón, Miguel Angel Moreno-Mateos

   Article

   Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

   Insert

   Human codon-optimized Cas9 variant SpG
   

   Use

   CRISPR

   Tags

   6xHis and SV40-NLS

   Expression

   Bacterial

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPN10

   Plasmid

   #114386

   Purpose

   Empty gRNA

   Depositor

   Vincent Dion

   Article

   Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBUN411

   Plasmid

   #50581

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   3×FLAG-NLS-zCas9-NLS
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG and NLS

   Mutation

   maize codon optimized

   Promoter

   OsU3p and Ubi1p

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHSN401

   Plasmid

   #50588

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   3×FLAG-NLS-zCas9-NLS
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG and NLS

   Mutation

   maize codon optimized

   Promoter

   2×35Sp and AtU6-26p

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSA_0_mKate2_synCoTC

   Plasmid

   #199469

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   mKate2
   

   Use

   CRISPR

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSA_2_mKate2_synCoTC

   Plasmid

   #199471

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   mKate2
   

   Use

   CRISPR

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSA_2_mTagBFP2_synCoTC

   Plasmid

   #199474

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   mTagBFP2
   

   Use

   CRISPR

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSA_0_mTagBFP2_synCoTC

   Plasmid

   #199472

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   mTagBFP2
   

   Use

   CRISPR

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSA_1_mKate2_synCoTC

    Plasmid

    #199470

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    mKate2
    

    Use

    CRISPR

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSA_1_mTagBFP2_synCoTC

    Plasmid

    #199473

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    mTagBFP2
    

    Use

    CRISPR

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKozak_mTagBFP2_synCoTC

    Plasmid

    #199493

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    mTagBFP2
    

    Use

    CRISPR

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDG459

    Plasmid

    #100901

    Purpose

    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-PuroR

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDG458

    Plasmid

    #100900

    Purpose

    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMpGE_En01

    Plasmid

    #71534

    Purpose

    Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

    Depositor

    Takayuki Kohchi, Ikuko Hara-Nishimura

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpG_ATH51-IP

    Plasmid

    #242074

    Purpose

    Expresses Cas9-SpG_ATH51 in mammalian cells

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

    Insert

    SpG_ATH51
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    ectopic insertion of 7 amino acids of PID turn-he…

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-Universal

    Plasmid

    #52694

    Purpose

    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

    Depositor

    Sebastian Lourido

    Inserts

    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    

    Use

    CRISPR; Toxoplasma gondii

    Tags

    3X FLAG and Nuclear localization signal

    Promoter

    TgTUB1 and Toxoplasma U6

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330 Mouse 3' Hp1a gRNA

    Plasmid

    #127898

    Purpose

    WT Cas9 Vector targeting the 3' end of the mouse Hp1a gene

    Depositor

    Mark Groudine

    Insert

    gRNA/Cas9
    

    Use

    CRISPR

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330 Mouse 5' Npm1 gRNA

    Plasmid

    #127900

    Purpose

    WT Cas9 Vector targeting the 5' end of the mouse Npm1 gene

    Depositor

    Mark Groudine

    Insert

    gRNA/Cas9
    

    Use

    CRISPR

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNA0306

    Plasmid

    #169452

    Purpose

    pRS415-TEF1p-Cas9-CYC1t; Cas9 expression plasmid

    Depositor

    Jef Boeke

    Article

    Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

    Insert

    Cas9
    

    Expression

    Yeast

    Promoter

    TEF1p

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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