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pGWB440

Plasmid

#74826

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB423

Plasmid

#74817

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB523

Plasmid

#74865

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB507

Plasmid

#74849

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2-FLAG-hEcto/Tif1g-CAmut

Plasmid

#20903

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (TRIM33 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Point mutagenesis of C125 and C128 to Alanine (RI…

Promoter

Available since

May 15, 2009

Availability

Academic Institutions and Nonprofits only
HypaCas9

Plasmid

#138557

Purpose

Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

HypaCas9

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

N692A, M694A, Q695A, H698A

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pWP040

Plasmid

#198777

Purpose

Drives specific expression in the ASG neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

PY41C4A.6-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCC12

Plasmid

#198767

Purpose

Drives specific expression in the ADF neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psrh-142-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pWP017

Plasmid

#198780

Purpose

Drives specific expression in the ASI neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pgpa-4-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB421

Plasmid

#74815

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 10xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pMM627

Plasmid

#112532

Purpose

pZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
ChuA
luxCDABE

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23107, PL(HrtO), and ProD

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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p53-(1-393)-R273H-N268D

Plasmid

#35087

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-393 - R273H-N268D

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
pGWB524

Plasmid

#74866

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with GST (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTR AE Clover

Plasmid

#90240

Purpose

Lentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter (AE mutant)

Use

Lentiviral

Tags

mClover

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB529

Plasmid

#74871

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with TAP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGAN200

Plasmid

#129203

Purpose

yTRAP sensor of [PSI+], detects aggregation of Sup35

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Sup35NM (SUP35 Budding Yeast)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

N terminal and middle domains

Promoter

SUP35

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB443

Plasmid

#74829

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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PLEX_313 GFP

Plasmid

#184494

Purpose

Lentivirus expression for GFP

Depositor

Peter Tsvetkov, Todd Golub

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-Lenti-CAGGS-hPRDM6-2xGly-V5-EF1-Luc2-Blast

Plasmid

#213826

Purpose

Expresses human PRDM6 with a C-terminal V5 epitope tag.

Depositor

Bjoern Schwer

Article

Schmidt et al bioRxiv. 2023 Aug 31:2023.08.29.555389.

Insert

PRDM6 (PRDM6 Human)

Use

Lentiviral and Luciferase

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2z-tal1

Plasmid

#164656

Purpose

for the synthesis of zebrafish tal1 mRNA

Depositor

Didier Stainier

Article

Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

Insert

tal1 (tal1 Zebrafish)

Use

Mrna synthesis

Tags

Expression

Mutation

Promoter

SP6

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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