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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 4461 - 4480 of 66674 results

  1. TPI-PTC1

    Plasmid
    #130699
    Purpose
    Expresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid)
    Depositor
    Marvin Tanenbaum
    Publication
    Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TPI-PTC1 (TPI1 Human)
    Expression
    Mammalian
    Mutation
    PTC1
    Promoter
    Pcmv
    Available since
    Sept. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    130699-plasmid-map-sequence-id-255908

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  3. pLenti Puro HA-Ubiquitin-K63

    Plasmid
    #236291
    Purpose
    Lentiviral vector expressing HA-tagged ubiquitin K63 mutant
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Ubiquitin K63 (UBB Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    Single ubiquitin coding sequence containing K63 m…
    Promoter
    CMV
    Available since
    April 10, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236291-plasmid-map-sequence-id-499303

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  7. pD649-HAsp-MAG-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156882
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Publication
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    MAG (MAG Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156882-plasmid-map-sequence-id-446217

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  9. HP21-TPI-PTC1

    Plasmid
    #131043
    Purpose
    Expresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid) with a 21 nt hairpin in 5'UTR
    Depositor
    Marvin Tanenbaum
    Publication
    Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TPI-PTC1 (TPI1 Human)
    Expression
    Mammalian
    Mutation
    PTC1
    Promoter
    Pcmv
    Available since
    Sept. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    131043-plasmid-map-sequence-id-255918

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  10. pLenti6-DJ1-V5-D149A

    Plasmid
    #29418
    Depositor
    Mark Cookson
    Insert
    DJ1 (PARK7 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Asp149Ala
    Available since
    April 15, 2011
    Availability
    Academic Institutions and Nonprofits only

  14. deltaLoop-MIDAS

    Plasmid
    #171605
    Purpose
    Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) without the loop region (a.a. 4458-4496) in bacteria.
    Depositor
    Tarun Kapoor
    Publication
    Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117
    Insert
    MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
    Tags
    His6 tag and SNAP tag
    Expression
    Bacterial
    Mutation
    Replaced amino acids 4458-4496 with ASGSGS linker
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    171605-plasmid-map-sequence-id-337861

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  15. pX330 EGFR-sgRNA

    Plasmid
    #188633
    Purpose
    A human codon-optimized SpCas9 and chimeric guide RNA targeting C-terminus of human EGFR
    Depositor
    Jared Toettcher
    Publication
    Farahani et al Elife. 2023 May 22;12:e82863. doi: 10.75
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Aug. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    188633-plasmid-map-sequence-id-369478

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  16. CFL1 (CFL1A-c002)

    Plasmid
    #98237
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CFL1A (CFL1 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00241:Cofilin_ADF 27-148
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98237-plasmid-map-sequence-id-193416

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  20. mRuby-EB3-7

    Plasmid
    #55858
    Purpose
    Localization: MT End Binding Protein, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EB3 (MAPRE3 Human)
    Tags
    mRuby
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 4, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 4461 - 4480 of 66674 results