We narrowed to 10,785 results for: pcdna3.1

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pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG

Plasmid

#127308

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-OTUB1 T134R,C91S-pcDNA3.1

Plasmid

#118212

Purpose

Expresses FLAG-OTUB1 T134R,C91S in mammalian cells

Depositor

Cynthia Wolberger

Publication

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Tags

3x FLAG

Expression

Mammalian

Mutation

changed cysteine 91 to serine and threonine 134 t…

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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Human NCKX2-AS-short-FlagTag (pcDNA3.1+)

Plasmid

#75202

Purpose

Mammalian expression of SLC24A2

Depositor

Jonathan Lytton

Publication

Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

Insert

SLC24A2 (SLC24A2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1-577-CFP-578CT

Plasmid

#41682

Depositor

Henry Lester

Publication

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)

Tags

CFP

Expression

Mammalian

Mutation

“Monomerizing” CFP A206K mutation; CFP inserted d…

Promoter

CMV

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1(+)mGAT1-577-YFP-578CT

Plasmid

#41681

Depositor

Henry Lester

Publication

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; YFP inserted d…

Promoter

CMV

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1-FLAG-SBP-STAG2 (S1215X)

Plasmid

#73970

Purpose

STAG2 cDNA (CCDS43990) S1215X

Depositor

Todd Waldman

Publication

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Tags

FLAG-SBP

Expression

Mammalian

Mutation

S1215X

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3.1-FLAG-SBP-STAG2 (Q1117X)

Plasmid

#73968

Purpose

STAG2 cDNA (CCDS43990) Q1117X

Depositor

Todd Waldman

Publication

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Tags

FLAG-SBP

Expression

Mammalian

Mutation

Q1117X

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3.1-FLAG-SBP-STAG2 (S1075X)

Plasmid

#73967

Purpose

STAG2 cDNA (CCDS43990) S1075X

Depositor

Todd Waldman

Publication

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Tags

FLAG-SBP

Expression

Mammalian

Mutation

S1075X

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3.1-FLAG-SBP-STAG2 (S653X)

Plasmid

#73965

Purpose

STAG2 cDNA (CCDS43990) S653X

Depositor

Todd Waldman

Publication

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Tags

FLAG-SBP

Expression

Mammalian

Mutation

S653X

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3.1-HaloAKAR2.1-NES-T2A-EGFP-CAAX

Plasmid

#216491

Purpose

Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and PM targeted EGFP in mammalian cells

Depositor

Jin Zhang

Publication

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.1

Tags

NES, T2A-EGFP-CAAX

Expression

Mammalian

Promoter

CMV

Available since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-myc-OGA(GS-544)C181-D174N

Plasmid

#193999

Purpose

plasmid encoding myc-tagged OGA(GS-544)C181-D174N

Depositor

Christina Woo

Publication

Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

Insert

myc-OGA(GS-544)C181-D174N

Tags

myc

Expression

Mammalian

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-iRFP-P2A-Post-SPHERE-SF-iGluSnFR

Plasmid

#187090

Purpose

Glutamate sensor for localization to membrane contact

Depositor

Kotaro Oka

Publication

Shindo et al bioRxiv 2022.04.03.486903; doi: https://

Insert

Post-SPHERE-SF-iGluSnFR

Tags

iRFP670-P2A

Expression

Mammalian

Promoter

CMV

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CARD8-TEV-GFP-FLAG S297A

Plasmid

#169992

Purpose

Mammalian purification construct for CARD8 with an autoprocessing-deficient mutation

Depositor

Daniel Bachovchin

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

TEV-siriusGFP-FLAG

Expression

Mammalian

Mutation

S297A

Promoter

CMV

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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(366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A)

Plasmid

#168189

Purpose

HA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutation

Depositor

Christina Woo

Publication

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-nUbc6E-(EAAAK)4-OGT(4,K852A)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL100EE

Plasmid

#166798

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. LL100EE mutant is loss of CARD8 and NLRP1 binding.

Depositor

Hao Wu

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Mutation

LL100EE

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S S730A

Plasmid

#166801

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. The S730A mutant is catalytically dead.

Depositor

Hao Wu

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Mutation

S730A

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

Plasmid

#163991

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Publication

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 CARD (NLRP1 Human)

Tags

mCherry

Expression

Mammalian

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS-mFndc3b Mutant miRNAs

Plasmid

#126444

Purpose

Expresses a mutated version of mouse Fndc3b circular RNA with the miR-93-3p, miR-258-5p, and miR-412-3p target sites all mutated

Depositor

Jeremy Wilusz

Publication

Garikipati et al Nat Commun. 2019 Sep 20;10(1):4317. doi:

Insert

Fndc3b (Fndc3b Mouse)

Expression

Mammalian

Mutation

circFndc3b exons are separated by a miniature int…

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-GFP 5' Half Only

Plasmid

#69907

Purpose

Expresses a circular RNA containing the 5' half of GFP in mammalian cells

Depositor

Jeremy Wilusz

Publication

Kramer et al Genes Dev. 2015 Oct 8.

Insert

GFP, ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc-mβTrCP WD2-7 (292-569)

Plasmid

#62978

Purpose

Expresses myc-tagged mouse βTrCP residues 292-569

Depositor

Michael Ruppert

Publication

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

BTRC (Btrc Mouse)

Tags

myc

Expression

Mammalian

Mutation

codes for residues 292-569

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only