We narrowed to 5,055 results for: cre encoding plasmid

1. 011LP-pAAV-TNT4-EmGFP-pA

   Plasmid

   #120280

   Purpose

   AAV vector for cardiomyocyte specific expression of Emerald Green Fluorescent Protein using cardiac troponin T promoter

   Depositor

   Xander Wehrens

   Article

   Ni et al Circ Res. 2019 Jan 18;124(2):256-262. do

   Insert

   EmGFP
   

   Use

   AAV

   Tags

   3XFlag and HA

   Expression

   Mutation

   Promoter

   TNT Cardiac Troponin T promoter

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. CDK1as_T2A_puromycin

   Plasmid

   #118595

   Purpose

   One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.

   Depositor

   William Earnshaw

   Article

   Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

   Insert

   CDK1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-SpCas9-2A-GFP-noITR

   Plasmid

   #118415

   Purpose

   Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

   Depositor

   Mark Denham

   Article

   Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. CDK1as_T2A_Zeo

   Plasmid

   #118596

   Purpose

   One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.

   Depositor

   William Earnshaw

   Article

   Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

   Insert

   CDK1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG/hTAZ-FL

   Plasmid

   #139979

   Purpose

   Expresses full length human Tafazzin with codon modified for cloning. Transgene flanked by AAV2 ITR

   Depositor

   William Pu

   Article

   Wang et al Circ Res. 2020 Mar 9. doi: 10.1161/CIRCR

   Insert

   Full length human Tafazzin (TAFAZZIN Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETDuet-solo-NHis6-NocO-D282A

   Plasmid

   #240407

   Purpose

   expresses N-His tagged NocO D282A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

   Depositor

   Emily Balskus

   Article

   Wang et al Biochemistry. 2025 May 20;64(10):2173-21

   Insert

   nocO
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   M1 of native sequence not included in insert, Asp…

   Promoter

   T7

   Available since

   Aug. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet-solo-NHis6-NocO-H286A

   Plasmid

   #240409

   Purpose

   expresses N-His tagged NocO H286A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

   Depositor

   Emily Balskus

   Article

   Wang et al Biochemistry. 2025 May 20;64(10):2173-21

   Insert

   nocO
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   M1 of native sequence not included in insert, His…

   Promoter

   T7

   Available since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETDuet-solo-NHis6-NocO-H132A

   Plasmid

   #240404

   Purpose

   expresses N-His tagged NocO H132A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

   Depositor

   Emily Balskus

   Article

   Wang et al Biochemistry. 2025 May 20;64(10):2173-21

   Insert

   nocO
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   M1 of native sequence not included in insert, His…

   Promoter

   T7

   Available since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETDuet-solo-NHis6-NocO-E129A

   Plasmid

   #240403

   Purpose

   expresses N-His tagged NocO E129A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

   Depositor

   Emily Balskus

   Article

   Wang et al Biochemistry. 2025 May 20;64(10):2173-21

   Insert

   nocO
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   M1 of native sequence not included in insert, Glu…

   Promoter

   T7

   Available since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETDuet-solo-NHis6-NocO-Q128A

    Plasmid

    #240402

    Purpose

    expresses N-His tagged NocO Q128A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocO
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M1 of native sequence not included in insert, Glu…

    Promoter

    T7

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETDuet-solo-NHis6-NocO-E252A

    Plasmid

    #240405

    Purpose

    expresses N-His tagged NocO E252A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocO
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M1 of native sequence not included in insert, Glu…

    Promoter

    T7

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETDuet-solo-NHis6-NocO-E283A

    Plasmid

    #240408

    Purpose

    expresses N-His tagged NocO E283A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocO
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M1 of native sequence not included in insert, Glu…

    Promoter

    T7

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETDuet-solo-NHis6-NocO-H279A

    Plasmid

    #240406

    Purpose

    expresses N-His tagged NocO H279A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocO
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M1 of native sequence not included in insert, His…

    Promoter

    T7

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pETDuet-solo-NHis6-NocO-E94A

    Plasmid

    #240401

    Purpose

    expresses N-His tagged NocO E94A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocO
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M1 of native sequence not included in insert, Glu…

    Promoter

    T7

    Available since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNKX2-5-GFP-T2A

    Plasmid

    #203608

    Purpose

    Donor plasmid to create 5' knockin of NKX2-5 with GFP-T2A

    Depositor

    Matthew Birket

    Article

    Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

    Insert

    NKX2-5-GFP-T2A-NKX2-5 (NKX2-5 Human)
    

    Use

    Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV/D377Y-mPCSK9

    Plasmid

    #58376

    Purpose

    Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

    Depositor

    Jacob Bentzon

    Article

    Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

    Has Service

    AAV8

    Insert

    proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    D377Y

    Promoter

    HCRApoE/hAAT

    Available since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMV306DIhsp+LuxG13

    Plasmid

    #49999

    Purpose

    Integrase removed to increase stability of the plasmid in mycobacteria

    Depositor

    Brian Robertson

    Article

    Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

    Insert

    Bacterial luciferase operon + G13 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    It contains a gram-positive enhanced translation …

    Promoter

    G13 from M. marinum G13 promoter driving luxC

    Available since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMV306DIG13+FFlucRT

    Plasmid

    #49998

    Purpose

    Integrase removed to increase stability of the plasmid in mycobacteria

    Depositor

    Brian Robertson

    Article

    Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

    Insert

    G13 promoter + Firefly luciferase Red Thermostable
    

    Use

    Mycobacteria integrating vector

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for Mycobacterium tuberculosis wi…

    Promoter

    G13

    Available since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. L2_lacZgRNA-Cas9-CsA

    Plasmid

    #136138

    Purpose

    Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-Flag-deSpCas9

    Plasmid

    #92114

    Purpose

    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    Cbh

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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