We narrowed to 8,994 results for: Ski

1. pEND-418_Cacnes_LysA

   Plasmid

   #225607

   Purpose

   Suicide C. acnes vector to generate LysA (PPA_RS06345) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEY139

   Plasmid

   #223486

   Purpose

   tph-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   tph-1 (tph-1 Nematode)
   

   Expression

   Worm

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEY82

   Plasmid

   #191077

   Purpose

   flp-7 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   flp-7 (flp-7 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTomo-Ras

   Plasmid

   #26292

   Depositor

   Inder Verma

   Article

   Marumoto et al Nat Med. 2009 Jan . 15(1):110-6.

   Insert

   HRAS-V12 (HRAS Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Mutation

   G12V

   Available Since

   Sept. 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. GAL FLAG72QDProGFP p306

   Plasmid

   #15833

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 72Q Delta Pro (HTT Human)
   

   Tags

   FLAG and GFP

   Expression

   Yeast

   Mutation

   Proline-rich region removed

   Available Since

   Oct. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

6. VinTS-tCRMod-GGSGGS7

   Plasmid

   #111764

   Purpose

   Expresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 module

   Depositor

   Brenton Hoffman

   Article

   LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

   Insert

   Clover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
   

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDF0191 CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

   Plasmid

   #172505

   Purpose

   Encodes the CjcCas7-11 protein only for bacterial recombinant protein expression for purification

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUAST td tomato Rab5 WT

   Plasmid

   #58446

   Purpose

   expression of drosophila td tomato Rab5

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab5 (Rab5 Fly)
   

   Tags

   td tomato

   Expression

   Insect

   Promoter

   GAL4

   Available Since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pDF0336 pAAV EFS-HvsCas7-11 for dual vector

   Plasmid

   #172515

   Purpose

   Encodes HvsCas7-11 mammalian expression casette in AAV backbone

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   pAAV EFS-HvsCas7-11 for dual vector
   

   Use

   AAV

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-BiP-Myc-KDEL P495L

    Plasmid

    #27166

    Depositor

    Ron Prywes

    Article

    Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

    Insert

    BiP (HSPA5 Human)
    

    Tags

    KDEL and Myc

    Expression

    Mammalian

    Mutation

    Peptide Binding Mutant: P495L

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

11. GAL 103Q+ProGFPp416

    Plasmid

    #15583

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt103Q + Pro (HTT Human)
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Proline-rich region present

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pCMV-BiP-Myc-KDEL T37G

    Plasmid

    #27165

    Depositor

    Ron Prywes

    Article

    Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

    Insert

    BiP (HSPA5 Human)
    

    Tags

    KDEL and Myc

    Expression

    Mammalian

    Mutation

    ATP bound form: T37G

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA-c/o NL4-3 Env delta CT

    Plasmid

    #139285

    Purpose

    HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    codon optimized HIV-1 Env delta cytoplasmic tail
    

    Expression

    Mammalian

    Mutation

    deletion of cytoplasmic tail after residue 710

    Promoter

    CMV IE

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2+ Gal4-GBP2-IRES-GBP7-p65

    Plasmid

    #50020

    Purpose

    Multi-purpose expression of a GFP-dependent transcription factor

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    Gal4DBD-GBP2-IRES-NLS-GBP7-p65AD
    

    Use

    Multi-purpose expression

    Promoter

    CMV-immediate early promoter and SP6 promoter

    Available Since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmKate2-MS2coatProtein

    Plasmid

    #103833

    Purpose

    Live cell reader for MS2 loop RNA production

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    mKate2-MS2 coat protein (cp )
    

    Expression

    Mammalian

    Mutation

    MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMB3-B-TelR

    Plasmid

    #204992

    Purpose

    Level 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

    Depositor

    Veronica Godoy-Carter

    Article

    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Added replications elements for Acinetobacter spe…

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pALPS_3'LTR GFP@gag start SFFV tat-P2A-rev

    Plasmid

    #101340

    Purpose

    LTR drives GFP and internal SFFV promoter drives codon optimized tat and rev.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    GFP, tat, rev
    

    Use

    Lentiviral

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-MBP-TEVpCS-ADGRB1 CTF-FLAG

    Plasmid

    #217681

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRB1 (ADGRB1 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-926

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-GBP1-10gly-Gal4DBD

    Plasmid

    #49438

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP1-Gal4 DNA binding domain fusion protein
    

    Expression

    Mammalian

    Mutation

    C98S (to increase protein solubility), Q3D

    Promoter

    CAG

    Available Since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET22b_EcSSB_1

    Plasmid

    #78203

    Purpose

    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

    Depositor

    Martin Webb

    Article

    Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1

    Insert

    ssb (G26C)
    

    Expression

    Bacterial

    Mutation

    G26C (please see depositor comment below)

    Promoter

    T7

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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