We narrowed to 8,512 results for: gnal

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pBSFI-myr-akt-T308D/S473D

Plasmid

#49192

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from T to D at position 308 and S to D at position 473 in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Mutation

T308D; S473D

Promoter

T7

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-B-AtAFB2-mCherry

Plasmid

#129718

Purpose

AAVS1 safe harbor targeting vector with blasticidin resistance gene expressing AtAFB2-mCherry

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK2 wt

Plasmid

#80870

Purpose

mammalian expression of ALK2 WT

Depositor

Aristidis Moustakas

Article

Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

Insert

ALK2 (ACVR1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-GFP11-Actin

Plasmid

#181966

Purpose

Human beta actin tagged with GFP11.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

GFP11-Actin (ACTB Human)

Tags

GFP11

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLJM60-FLAG-SLC38A9.1

Plasmid

#71858

Purpose

lentiviral stable expression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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CMV-10-3xFLAG-LGP2

Plasmid

#58681

Purpose

Expresses 3x Flag-tagged LGP2 in mammalian cells

Depositor

Curt Horvath

Article

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

laboratory of genetics and physiology 2 (DHX58 Human)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2014

Availability

Academic Institutions and Nonprofits only

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pH1R-P2A-mCherry-N1

Plasmid

#84330

Purpose

Co-expresses (untagged) Histamine-1 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H1R-P2A-mCherry (HRH1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSG5-HA-p300-DY1399

Plasmid

#89095

Purpose

mammalian expression of full-length human p300 with DY1399 mutation, acetylation mutant, acetyltransferase activity defect

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300-D1399Y mutant (EP300 Human)

Tags

HA

Expression

Mammalian

Mutation

p300-DY1399

Promoter

SV40

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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GAL-p300-230-670

Plasmid

#89099

Purpose

mammalian expression of human p300 amino acids 230-670 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 230-670

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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POLD1_pLX307

Plasmid

#98358

Purpose

Lentiviral expression of POLD1

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

POLD1 (POLD1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-hARdeltaNH2-6

Plasmid

#89104

Purpose

mammalian expression of human androgen receptor amino acids 507-919

Depositor

Elizabeth Wilson

Article

Simental et al J Biol Chem. 1991 Jan 5;266(1):510-8.

Insert

hAR DNA binding domain+ligand binding domain (AR Human)

Expression

Mammalian

Mutation

NH2 terminal deletion, contains amino acids 507-…

Promoter

CMV

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pLL5.0-MRLC-AA-GFP

Plasmid

#187259

Purpose

Lentiviral expression of myosin regulatory light chain-AA mutant

Depositor

Alpha Yap

Article

Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

Insert

MRLC1 (MYL9 Human)

Use

Lentiviral

Tags

EGFP

Mutation

T18A,S19A

Promoter

5' LTR

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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GAL-p300-1601-1900

Plasmid

#89102

Purpose

mammalian expression of human p300 amino acids 1601-1900 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 1601-1900

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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GAL-p300-501-999

Plasmid

#89100

Purpose

mammalian expression of human p300 amino acids 501-999 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 501-999

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pEF-Bos-FLAG RIG-I MIII

Plasmid

#52877

Purpose

Expresses RIG-I with a mutation to motif III in mammalian cells

Depositor

Curt Horvath

Article

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

DDX58 (RIGI Human)

Tags

FLAG

Expression

Mammalian

Mutation

T409A, S411A

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

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pCDEF3‐hTIM3 (no tag)

Plasmid

#49212

Purpose

expresses human Tim3

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 (HAVCR2 Human)

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pAd-Wnt3a

Plasmid

#12518

Depositor

Tong-Chuan He

Article

Luo et al J Biol Chem. 2004 Dec 31. 279(53):55958-

Insert

Wnt3A (Wnt3a Mouse)

Use

Adenoviral

Expression

Mammalian

Available Since

Nov. 13, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-ArgiNLS-mGreenLantern

Plasmid

#220596

Purpose

Constitutive expression of a single-cell discriminating version of mGreenLantern fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mGreenLantern

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloAKAR2.1-NES-T2A-EGFP-CAAX

Plasmid

#216491

Purpose

Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and PM targeted EGFP in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.1

Tags

NES, T2A-EGFP-CAAX

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pME map2k2a_s219D

Plasmid

#201058

Purpose

Gateway middle entry vector expressing constitutively active zebrafish map2k2a

Depositor

Sarah Childs

Article

Greysson-Wong et al Development. 2023 Sep 15;150(18):dev2018

Insert

MAP2k2a (map2k2a Zebrafish)

Use

Zebrafish expression

Mutation

Mutation S219D, constitutively active

Promoter

none

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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