We narrowed to 14,416 results for: nts

1. pCBC-DT3T4

   Plasmid

   #50592

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T3, gRNA scaffold, U6-1tplus, U6-26p, T4
   

   Use

   CRISPR; Pcr template

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA

   Plasmid

   #63166

   Purpose

   GAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-HA"
   

   Use

   Tags

   N-myristoylation

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cas9 TLS2

   Plasmid

   #196978

   Purpose

   Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Cas9 with TLS2 tag
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pG10-90

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTBL3478 PB-PEmax-mTagBFP2

   Plasmid

   #226664

   Purpose

   PiggyBac cargo vector encoding PEmax and mTagBFP2

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Inserts

   PEmax
   mTagBFP2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and UBC

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. tubP-FRT>GAL80-6-FRT>

   Plasmid

   #63173

   Purpose

   ubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassette

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "Flp-Out" GAL80
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD-BirA-eCPX-AP

   Plasmid

   #121907

   Purpose

   Expresses BirA and eCPX-AP

   Depositor

   Per Svenningsen

   Article

   Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

   Inserts

   BirA (birA Synthetic)
   Enhanced Circularly Permuted Outer Membrane Protein X
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 and pBAD promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiPGK DEST H2B-iRFP670

   Plasmid

   #90237

   Purpose

   Lentiviral vector to express H2B-iRFP670 under PGK promoter

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   H2B
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGR

   Plasmid

   #46002

   Purpose

   Reporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinose

   Depositor

   Christopher Voigt

   Article

   Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   June 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1 Lifeact-EGFP-2XNLS

   Plasmid

   #58467

   Purpose

   Expresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

   Inserts

   Lifeact
   2X NLS with 2X stop codons
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAGM47523

    Plasmid

    #153221

    Purpose

    Level 0 zCas9i vector

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    SpCas9 with introns and 2 NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI-neo Fluc EGFP

    Plasmid

    #90170

    Purpose

    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins

    Depositor

    Franz-Ulrich Hartl

    Article

    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

    Insert

    FLuc EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDGE332

    Plasmid

    #153241

    Purpose

    M1 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDGE333

    Plasmid

    #153242

    Purpose

    M2 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFG2

    Plasmid

    #87250

    Purpose

    gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-Nat

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    Nat
    

    Use

    Tags

    parS segments

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGE335

    Plasmid

    #153244

    Purpose

    M3 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro

    Plasmid

    #31482

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Traffic Light Reporter 1.1 (Sce Target) EF Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    mCherry has M9S and M16L mutations. This is what …

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. CP1041-ABEmax

    Plasmid

    #119808

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (CP1041)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGE496

    Plasmid

    #153248

    Purpose

    M6 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDGE495

    Plasmid

    #153247

    Purpose

    M5 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDGE336

    Plasmid

    #153245

    Purpose

    M4 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge